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Skin benefits of postbiotics derived from Micrococcus luteus derived from human skin: an untapped potential for dermatological health

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Abstract

Background

The skin microbiome, a diverse community of microorganisms, plays a crucial role in maintaining skin health. Among these microorganisms, the gram-positive bacterium Micrococcus luteus exhibits potential for promoting skin health. This study focuses on postbiotics derived from M. luteus YM-4, a strain isolated from human skin.

Objective

Our objective is to explore the beneficial effects of YM-4 culture filtrate on dermatological health, including enhancing barrier function, modulating immune response, and aiding recovery from environmental damage.

Methods

The effects of the YM-4 culture filtrate were tested on human keratinocytes and fibroblasts under various conditions using real-time PCR for gene expression analysis and fibroblast migration assays. A dehydration-simulated model was employed to prepare RNA-Seq samples from HaCaT cells treated with the YM-4 culture filtrate. Differentially expressed genes were identified and functionally classified through k-means clustering, gene ontology terms enrichment analyses, and protein-protein interactions mapping.

Results

The YM-4 culture filtrate enhanced the expression of genes involved in skin hydration, hyaluronic acid synthesis, barrier function, and cell proliferation. It also reduced inflammation markers in keratinocytes and fibroblasts under stress conditions. It mitigated UVB-induced collagen degradation while promoted collagen synthesis, suggesting anti-aging properties, and accelerated wound healing processes by promoting cell proliferation and migration. RNA sequencing analysis revealed that the YM-4 culture filtrate could reverse dehydration-induced transcriptional changes towards a state similar to untreated cells.

Conclusion

M. luteus YM-4 culture filtrate exhibits significant therapeutic potential for dermatological applications.

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Acknowledgements

The authors gratefully acknowledge Center for Bio-Medical Engineering Core Facility at Dankook University for providing critical reagents and equipment. This project was supported by COSMAX BTI, Republic of Korea.

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Not applicable.

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Author notes

  1. Seyoung Mun

    Present address: Center for Bio Medical Engineering Core Facility, Dankook University, Cheonan, 31116, Republic of Korea

  2. Young Mok Heo and Dong-Geol Lee contributed equally to this work.

Authors and Affiliations

  1. R&I Center, COSMAX BTI, Seongnam, 13486, Republic of Korea

    Young Mok Heo, Dong-Geol Lee, Minji Kim, Chaeyun Baek, Haeun Lee, Seok Kyun Yun & Seunghyun Kang

  2. Department of Microbiology, College of Science & Technology, Dankook University, Cheonan, 31116, Republic of Korea

    Dong-Geol Lee, Seyoung Mun & Kyudong Han

  3. Center for Bio Medical Engineering Core Facility, Dankook University, Cheonan, 31116, Republic of Korea

    Kyudong Han

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  1. Young Mok Heo
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Correspondence to Kyudong Han.

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The authors declare they have no financial interests.

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Informed consent was obtained from all individual participants included in the study.

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Y.M. Heo, D.-G. Lee, M. Kim, C. Baek, H. Lee, S.K. Yun, S. Kang, and are employed by COSMAX BTI.

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Heo, Y.M., Lee, DG., Mun, S. et al. Skin benefits of postbiotics derived from Micrococcus luteus derived from human skin: an untapped potential for dermatological health. Genes Genom 46, 13–25 (2024). https://doi.org/10.1007/s13258-023-01471-w

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