Abstract
The top-mouth culter Erythroculter ilishaeformis is a kind of carnivorous fish that is currently a widely distributed fish in China, which is a commercially important freshwater fish in China. However, only few effective molecular markers on E. ilishaeformis are available. In this study, nine muscles and liver tissues of cDNA libraries of E. ilishaeformis were sequenced using Illumina HiSeq4000 paired-end sequencing technology. 86,574 sequences from 80,945,107 paired-end reads were generated. 55 novel simple sequence repeat (SSR) markers were developed based on high-throughput sequencing. After genetic diversity analysis, observed heterozygosity (Ho) was from 0.1613 to 0.4839. Expected heterozygosity (He) was from 0.1507 to 0.3728. Minor allele frequencies (MAFs) were from 0.3333 to 0.5000, and Hardy-Weinberg values were relatively balanced. These SSR markers developed from transcriptome sequencing will be as a useful tool to exploit populations genetic of E. ilishaeformis.
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Acknowledgements
The authors would like to express their contribution to the contribution made by Shanghai Meiji Biological Co.
Funding
This work was supported by University Natural Science Research Project of Anhui Province, China (KJ2019ZD59); the Key Research and Development Plan Project of Anhui Province, China (1804a07020119); the Special Fund for Anhui Agriculture Research System, China.
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HX: Investigation, Data curation, Methodology, Formal analysis and Writing—original draft. DJ and BG: Investigation, Methodology, Formal analysis. MX: Data curation, Formal analysis, Methodology, Software, Writing—original draft, Writing review & editing. All authors reviewed the manuscript. All authors read and approved the final manuscript for submission.
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Investigations and protocols were conducted according to the guiding principles for the use and care of laboratory animals and in compliance with Huainan Normal University Institute of Animal Care and Use Committee. The institutional review board approved this procedure. Our study had been submitted to and approved by the Academic Ethics Committee of Huainan Normal University. All sample collection was undertaken in accordance with relevant Academic Ethics Committee of Huainan Normal University guidelines and regulations.
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Xu, H., Gong, B., Ju, D. et al. Development of SSR markers in top-mouth culter (Erythroculter ilishaeformis) based on high-throughput sequencing. Conservation Genet Resour 16, 17–22 (2024). https://doi.org/10.1007/s12686-023-01336-0
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DOI: https://doi.org/10.1007/s12686-023-01336-0