Abstract
The toxicity of chemical substances to algal growth is generally measured by the 72–96 h algal growth inhibition test. We have developed a method to assess the toxicity of chemicals in aquatic environments more quickly and simply than conventional testing methods by delayed fluorescence (DF), which reflects the photosynthetic capacity of algae. The DF method is based on a technique for evaluating the amount of change in the decay curve due to the effects of chemicals (\({\varDelta}{{{\mathrm{DF}}}}\), DF inhibition). Various studies on DF have been reported; however, few reports have evaluated the decay curve of DF by approach using inductive modeling based on measurement data such as principal component analysis (PCA) and partial least squares regression analysis (PLS). Therefore, the purpose of this study is to examine methods for estimating the magnitude and type of toxicity of chemicals by means of a principal component model (PC model) and multiple regression model (MR model) derived from changes in the decay curves of DF of algae exposed to a wide range of 37 toxic substances that have an effect of clear magnitude on algal growth. The changes in the DF decay curves due to exposure the 37 toxic substances to algae were summarized in the PC model composed of eigenvectors and scores of four principal components. For validation of usefulness, a hierarchical cluster analysis (HCA) of the amount of change in four PC scores revealed that the growth inhibition rate was more influential than the chemical type. We also found the possibility of quantitatively predicting the growth inhibition of chemicals by MR model by the amount of change in the PC scores.
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References
Breuer L, Doren L, Ebke K (2016) Comparison of four measuring techniques to assess growth inhibition standardized tests with seven freshwater algae and cyanobacteria. Toxicol Environ Chem 98:848–859
Dabrowski P, Kalaji MH, Baczewska AH, Pawluśkiewicz B, Mastalerczuk G, Borawska-Jarmułowicz B, Paunov M, Goltsev V (2017) Delayed chlorophyll a fluorescence, MR 820, and gas exchange changes in perennial ryegrass under salt stress. J Luminesc 183
Dabrowski P, Baczewska-Dabrowska AH, Bussotti F, Pollaustrini M, Piekut K, Kowalik W, Wrobel J, Kalaji HM (2021) Photosynthetic efficiency of Microcystis ssp. under salt stress. Environ Exp Botany 186:104459–104470
Gerhardt V, Krause H (1984) Delayed fluorescence of algae. J Luminesc 31–32:895–898
Ikushima Y, Takeuchi A, Katsumata M, Sato Y, Hakamata T (2020) Modeling and estimation of harmful substances by statistical analysis of delayed luminescence decay curves obtained from green algae Raphidocelis subcapitata. J Luminesc 223:117209
Jursinic PA (1986) Delayed fluorescence: Current concepts and status. Light Emission Plants Bact 291–328
Katsumata M, Koike T, Nishikawa M, Kazumura K, Tsuchiya H (2006) Rapid ecotoxicological bioassay using delayed fluorescence in the green alga Pseudokirchneriella subcapitata. Water Res 40:3393–3400
Katsumata M, Takeuchi A, Kazumura K, Koike T (2008) New feature of delayed luminescence: Preillumination-induced concavity and convexity in delayed luminescence decay curve in the green alga Pseudokirchneriella subcapitata. J Photochem Photobiol B: Biol 90:152–162
Katsumata M, Ikushima Y, Bennett K, Sato Y, Takeuchi A, Tatarazako N, Hakamata T (2017) Validation of rapid algal bioassay using delayed fluorescence in an interlaboratory ring study. Sci Total Environ 605–606:842–851
Kobori H, Tsuchikawa S (2013) Time-resolved principal component imaging analysis of chlorophyll fluorescence induction for monitoring leaf water stress. Appl Spectrosc 67–6
Leunert F, Grossart H, Gerhardt V, Eckert W (2013) Toxicant induced changes on delayed fluorescence decay kinetics of cyanobacteria and green algae: A rapid and sensitive biotest. Plos One 8:e63127
Matsunaka S (1964) Chemical Structure and Mode of Action of Phenol Herbicides. Weed Res 40–45.
Munekage Y, Hojo M, Meurer J, Endo T, Tasaka M, Shikanai T (2002) PGR5 is involved in cyclic electron flow around photosystem I is essential for photoprotection in Arabidopsis. Cell 110:361–371
Munekage Y, Shikanai T (2005) Cyclic electron transport through photosystem I. Plant. Biotechnol. 22:361–369
Naito K (1997) Pollutant Release and Transfer Register (PRTR) system. Waste Manag Res 8(2):107–112
Nozawa M (2012) Introduction to multivariate analysis by JUSE-StatWorks, JUSE Press, Ltd. Hieralchical Cluster Analysis (Chapter 9), Japan
OECD (2011) Guideline for testing of chemicals No.201. freshwater alga and cyanobacteria, growth inhibition test. Adopted: 23 March 2006, Annex 5 corrected: 28 July 2011, Organization for Economic Cooperation and Development, Paris, France
Rokach L, Maimon O (2010) Clustering Methods. Date Mining and Knowledge Discovery Handbook, Clustering methods (Chapter 15) https://www.cs.swarthmore.edu/~meeden/cs63/s16/reading/Clustering.pdf
Strehler BL, Arnold W (1951) Light production by green plants. J General Physiol 34:809–820
Scordino A, Triglia A, Musumeci F, Grasso F, Raifur Z (1996) Influence of the presence of atrazine in water on the in-vivo delayed luminescence of Acetabularia acetabulum. J Photochem Photobiol B: Biol 32:11–17
Smith LI (2002) A tutorial on Principal Components Analysis. Principal component analysis (Chapter 3) https://www.iro.umontreal.ca/~pift6080/H09/documents/papers/pca_tutorial.pdf
Shixuan H, Xie W, Zhang P, Fang S, Li Z, Tang P, Gao X, Guo GJ, Tolili C, Wang D (2018) Preliminary identification of unicellular algal genus by using combined confocal resonance Raman spectroscopy with PCA and DPLS analysis. Spectrochim Acta Part A: Mol Biomol Spectrosc 190:417–422
Taiz L, Zeiger E (2002) Plant Physiology third edition, Sinauer Associates, Inc. Photosynthesis: The light reactions (Chapter 7), Blue-light responses: Stomatal movements and morphogenesis (Chapter 8)
Yamagishi T, Katsumata M, Yamaguchi H, Shimura Y, Kawachi M, Koshikawa H, Horie Y, Tatarazako N (2016) Rapid ecotoxicological bioassay using delayed fluorescence in the marine cyanobacterium Cyanobium sp. (NIES-981). Ecotoxicology 25:1751–1758
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AT wrote the main manuscript. YI was involved in data analysis. MK, YS, and TH advised the manuscript. All authors reviewed the manuscript.
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Takeuchi, A., Ikushima, Y., Katsumata, M. et al. Quantitative prediction of the growth inhibition of various harmful chemicals by statistical analysis of delayed fluorescence decay curves obtained from the green alga Raphidocelis subcapitata. Ecotoxicology 32, 1174–1186 (2023). https://doi.org/10.1007/s10646-023-02711-1
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DOI: https://doi.org/10.1007/s10646-023-02711-1