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A colorimetric aptasensor for detecting ochratoxin A based on label-free aptamer and gold nanozyme

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Abstract

In this study, the aptamer of ochratoxin A (OTA) increased the negative charge density on the surface of gold nanoparticles (AuNPs) and promoted the release of hydroxyl radicals and Au3+ to enhance the peroxidase-like activity of the AuNPs. The OTA bound only to the aptamer and did not adsorb non-specifically to the AuNPs. Based on these two conclusions, a label-free colorimetric aptasensor was successfully developed, enabling the precise detection of OTA within the concentration range of 10–600 nM, with a remarkably low detection limit of 6.20 nM. The colorimetric aptasensor was applied to detect OTA in oats, corn, soybeans, rice, and glutinous rice.

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Acknowledgements

This work was financially supported by funding from the National Natural Science Foundation of China (31672605).

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Author notes

  1. Jidong Tang and Bing Tian have contributed equally to this work.

Authors and Affiliations

  1. College of Food Science, Southwest University, Chongqing, 400715, People’s Republic of China

    Jidong Tang, Bing Tian & Xiaoqi Tao

  2. Chongqing Key Laboratory of Speciality Food Co-Built by Sichuan and Chongqing, Chongqing, 400715, People’s Republic of China

    Xiaoqi Tao

Authors
  1. Jidong Tang
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  2. Bing Tian
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  3. Xiaoqi Tao
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Correspondence to Xiaoqi Tao.

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The authors state no conflict of interest.

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Tang, J., Tian, B. & Tao, X. A colorimetric aptasensor for detecting ochratoxin A based on label-free aptamer and gold nanozyme. ANAL. SCI. 39, 1623–1626 (2023). https://doi.org/10.1007/s44211-023-00404-7

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  • DOI: https://doi.org/10.1007/s44211-023-00404-7

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