Abstract
A self-pollinated, annual, and nutrient-rich legume, Lens tomentosus has excellent economic value since seeds of L. tomentosus are used all over the world. Chloroplast genome provides a valuable resource for phylogenetic reconstruction and divergence between legume species because of its maternal inheritance and low rearrangement rate. L. tomentosus complete chloroplast genome sequence was revealed through next-generation sequencing in this study. The length of L. tomentosus chloroplast genome was found to be 123,548 bp. The annotated genome carries 107 genes, 77 of which were protein-coding, 4 rRNA, and 27 tRNA genes. While other legume species contain two inverted repeat regions, two Lens species, L. tomentosus, and L. culinaris, have only one which classifies them in the IRLC clade. In addition, 66 simple sequence repeats (SSRs) were found in the L. tomentosus chloroplast genome, and multiple sequence alignment analysis revealed SSR polymorphisms unique to L. tomentosus. As a result, certain genic regions, such as ycf1 and paf1 (ycf3), can be potential markers for cultivar identification in future studies. This study will provide genomic data resources to the lentil genetics as it sequenced the first complete chloroplast genome L. tomentosus and compared it with L. culinaris chloroplast genome.
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Data availability
Raw sequencing and complete cp genomes annotations are publicly available at the ENA database (accession number: PRJEB47534, sample identification number: ERR6787436).
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We thank the Ege University Scientific Research Projects Coordinatorship (EGE-BAP) for funding the project under the FOA-2020-20981 project number.
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Bozkurt, A., Kaymaz, Y., Ateş, D. et al. The complete sequence of Lens tomentosus chloroplast genome. Acta Physiol Plant 46, 2 (2024). https://doi.org/10.1007/s11738-023-03628-2
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DOI: https://doi.org/10.1007/s11738-023-03628-2