Abstract
Low temperature and cold damage are natural factors that seriously reduce wheat yield. Thus, how to improve the cold resistance of wheat has been the focus of wheat breeders and geneticists. However, the genetic improvement for this trait has been slow, mainly because cold resistance is a complex quantitative trait and field phenotypic identification is relatively difficult. Therefore, the discovery, mapping, and cloning of the cold resistance genes of wheat provide a theoretical basis for the genetic improvement of wheat against cold resistance and facilitate the analysis of the molecular mechanisms of cold resistance in wheat. This study used the wheat line H261 and its EMS mutants LF2099 and XiNong 239 as materials. Cold trait segregation occurred in the F2 generation of mutants LF2099 and XiNong 239 at a 15:1 separation ratio. Genetic analysis showed that two dominant overlapping genes, temporarily named Wcr-3 and Wcr-4, control cold resistance in wheat. Furthermore, a combined BSA and SNP array established that Wcr-3 is between BU100519 (SSR marker) and AX-94843669 (SNP marker). The markers are 1.32 cM apart, corresponding to the 5.41 Mb physical interval on the Chinese Spring 2B chromosome with 67 functionally annotated genes. Wcr-4 is located between AX-94657955 (SNP marker) and LC-23 (SSR marker), which are 1.79 cM apart, corresponding to a 2.35 Mb physical interval on the Chinese Spring 2D chromosome, which contains 66 functionally annotated genes. Wcr-3 and Wcr-4 are two new cold resistance genes, laying the foundation for their fine mapping and cloning.
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The datasets generated and/or analyzed during the current study are available from Yanzhou Xie on reasonable request.
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Acknowledgements
This study was supported by the Key Program of Shaanxi Agricultural Cooperative Innovation and Promotion Alliance (No. LMZD202104), Creation and Application of High Quality and Powdery Mildew Resistance New Wheat Cultivar in South Huang-Huai (NK2022060503), Program of Introducing Talents of Innovative Discipline to Universities (Project 111) from the State Administration of Foreign Experts Affairs (#B18042) “Crop breeding for disease resistance and genetic improvement,” and Chinese Universities Scientific Fund (2452022112). The authors are also grateful to Liqun Li for her invaluable technical assistance and the two anonymous reviewers for their help in improving the manuscript.
Funding
This study was supported by the Key Program of Shaanxi Agricultural Cooperative Innovation and Promotion Alliance (No. LMZD202104), Creation and Application of High Quality and Powdery Mildew Resistance New Wheat Cultivar in South Huang-Huai (NK2022060503), Program of Introducing Talents of Innovative Discipline to Universities (Project 111) from the State Administration of Foreign Experts Affairs (#B18042) “Crop breeding for disease resistance and genetic improvement,” and Chinese Universities Scientific Fund (2452022112).
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C.L. and Y.Z.X. designed the experiments and critically revised the manuscript. C.L., M.Z.L., Z.P.C., and X.J.L. analyzed the data. C.L. wrote the manuscript. W.H., B.L., and S.Q.L. carried out the experiments. All authors have read and approved the manuscript.
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Lei, C., Li, M., Chen, Z. et al. Molecular mapping of two novel cold resistance genes in common wheat by 660K SNP array. Mol Breeding 43, 83 (2023). https://doi.org/10.1007/s11032-023-01425-w
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DOI: https://doi.org/10.1007/s11032-023-01425-w