Abstract
Red sea bream is a commercially valuable fish that is used as ingredients in the food industry. To prevent a decrease in fishing yield, farmed red sea bream is released. However, this may cause a decrease in the genetic diversity of wild populations, so systematic resource management is required. In this study, 68 single nucleotide polymorphism (SNP) markers were developed using a genotyping-by-sequencing (GBS) analysis. The minor allele frequency (MAF) ranged from 0.1802 to 0.5000, the observed heterozygosity (Ho) ranged from 0.0864 to 0.5000, expected heterozygosity (He) ranged from 0.2955 to 0.5000, and the polymorphism information content (PIC) ranged from 0.2518 to 0.3750. Of these SNPs, 25 loci significantly deviated from the Hardy–Weinberg equilibrium after a Bonferroni correction (p < 0.00074). These SNP markers will be useful for the further genetic analysis of the red sea bream population.
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Data availability
The data generated and analysed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
This research was supported by the Korea Institute of Marine Science and Technology Promotion (KIMST), funded by the Ministry of Oceans and Fisheries (20220572).
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This research was supported by the Korea Institute of Marine Science and Technology Promotion (KIMST), funded by the Ministry of Oceans and Fisheries (20220572).
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All authors contributed to the study conception and design. Sample preparation, data collection and analysis were performed by JGK, SHY, YBS and J-OK. The first draft of the manuscript was written by JGK and all authors read and approved the final manuscript. JGK and SHY have contributed equally.
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Kim, J., Yoo, S., Seo, Y. et al. Development and characterization of 68 SNP markers in red sea bream, Pagrus major. Conservation Genet Resour 16, 39–43 (2024). https://doi.org/10.1007/s12686-023-01337-z
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DOI: https://doi.org/10.1007/s12686-023-01337-z