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Self-Assembly Behavior and Cytotoxicity of PEG-b-PLA Nanoparticles for Improved Oxaliplatin Delivery: Effect of PLA Block Length

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Abstract

The present study investigates the effect of the length of the hydrophobic poly(D,L-lactide) block on the self-assembly behavior of amphiphilic poly(D,L-lactide)-b-poly(ethylene glycol) (P(D,L)LA-b-PEG5k) copolymers with a large variation in molecular weight of the hydrophobic block (ranging from 5 to 100 kDa). To evaluate the cytotoxic effect of oxaliplatin-loaded P(D,L)LA-b-PEG5k nanoparticles (OXA-NPs) prepared by the nanoprecipitation method, a half maximal inhibitory concentration (IC50) study was conducted in cancer cell lines (MCF7, HCT116, and A549). The assays revealed that the IC50 values for OXA-NPs in HCT116 (1.93 ± 0.08 nM) and A549 (2.84 ± 0.11 nM) were significantly lower than those of pure OXA (12.70 ± 1.14 and 29.30 ± 0.40 nM, respectively). Moreover, the results showed that normal fibroblasts treated with blank NPs exhibited no significant growth inhibition in the concentration range of 0.001–1 mg mL–1, indicating their high biocompatibility.

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ACKNOWLEDGMENTS

TEM and DLS measurements were performed in Resource Centers of the National Research Center “Kurchatov Institute.”

Funding

The research was supported by the Russian Science Foundation (project no. 18-73-10079-P).

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Authors and Affiliations

  1. National Research Center “Kurchatov Institute”, 123182, Moscow, Russia

    Y. Puchkova, N. Sedush, E. Kuznetsova & S. Chvalun

  2. Department of Chemistry, Moscow State University, 119991, Moscow, Russia

    A. Nazarov

Authors
  1. Y. Puchkova
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  2. N. Sedush
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  3. E. Kuznetsova
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  4. A. Nazarov
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  5. S. Chvalun
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Corresponding authors

Correspondence to Y. Puchkova or N. Sedush.

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Puchkova, Y., Sedush, N., Kuznetsova, E. et al. Self-Assembly Behavior and Cytotoxicity of PEG-b-PLA Nanoparticles for Improved Oxaliplatin Delivery: Effect of PLA Block Length. rev. and adv. in chem. 13, 152–159 (2023). https://doi.org/10.1134/S2634827623600056

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  • DOI: https://doi.org/10.1134/S2634827623600056

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