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Impact of Elevational Gradients and Chemical Parameters on Changes in Soil Bacterial Diversity Under Semiarid Mountain Region

  • Microbial Genetics, Genomics and Molecular Biology
  • Published:
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Abstract

Elevation gradients, often regarded as “natural experiments or laboratories”, can be used to study changes in the distribution of microbial diversity related to changes in environmental conditions that typically occur over small geographical scales. We obtained bacterial sequences using MiSeq sequencing and clustered them into operational taxonomic units (OTUs). The total number of reads obtained by the bacterial 16S rRNA sequencing analysis was 1,090,555, with an average of approximately 45,439 reads per sample collected from various elevations. The current study observed inconsistent bacterial diversity patterns in samples from the lowest to highest elevations. 983 OTUs were found common among all the elevations. The most unique OTUs were found in the soil sample from elevation_2, followed by elevation_1. Soil sample collected at elevation_6 had the least unique OTUs. Actinobacteria, Protobacteria, Chloroflexi were found most abundant bacterial phyla in current study. Ammonium nitrogen (NH4+-N), and total phosphate (TP) are the main factors influencing bacterial diversity at elevations_1. pH was the main factor influencing the bacterial diversity at elevations_2, elevation_3 and elevation_4. Our results provide new visions on forming and maintaining soil microbial diversity along an elevational gradient and have implications for microbial responses to environmental change in semiarid mountain ecosystems.

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Acknowledgements

This work was supported by the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant no. XDA 20100101), Major Special Science and Technology Project of Gansu Province (Grant no. 18ZD2FA009) and National Natural Science Foundation of China, NSFC (Grant no. 31522013).

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  1. State Key Laboratory of Grassland Agro-Ecosystems, School of Life Sciences, Lanzhou University, Lanzhou, 730000, Gansu, People’s Republic of China

    Salman Khan, Chun Han, Awais Iqbal, Chao Guan & Changming Zhao

  2. Gansu Provincial Field Scientific Observation and Research Station of Mountain Ecosystems, Lanzhou, 730000, Gansu, People’s Republic of China

    Salman Khan, Chun Han, Chao Guan & Changming Zhao

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  1. Salman Khan
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SK designed the study. SK, CH collected soil samples. SK, AI analyze the data, CG and CZ supervised.

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Correspondence to Changming Zhao.

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Khan, S., Han, C., Iqbal, A. et al. Impact of Elevational Gradients and Chemical Parameters on Changes in Soil Bacterial Diversity Under Semiarid Mountain Region. J Microbiol. 61, 903–915 (2023). https://doi.org/10.1007/s12275-023-00085-x

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