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Investigating hydrophobic environment in alkyl-group-functionalized silica particle with various chain lengths using absorption microspectroscopy

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Abstract

A well-known solvatochromic dye, Reichardt’s dye (R-dye), was used to evaluate the hydrophobicity of alkyl-group-functionalized silica particles (ASPs) with different chain lengths. The absorption spectra of R-dye were measured in a single ASP in a mixed solution of water and an organic solvent (methanol (MeOH), ethanol (EtOH), acetonitrile (ACN), tetrahydrofuran (THF), or N,N-dimethylformamide (DMF)) using absorption microspectroscopy. The polarity parameter in the ASPs (ET), determined by the absorption maximum, was observed to be smaller than those in bulk solutions, indicating that R-dye was present in a more hydrophobic environment. In EtOH, THF, and DMF, R-dye was distributed within the alkyl chain layer including the organic solvent. An increase in the organic solvent content of the bulk solution led to a higher organic solvent concentration in the alkyl chain layer, resulting in a decrease in ET. In MeOH and ACN, the R-dye was distributed within the alkyl chain layer and concentrated phase. Moreover, with the increase in the organic molecule content, the distribution of R-dye in the concentrated phase became dominant in MeOH and ACN system, leading to an increase in the ET value. The findings presented in this paper are expected to attract the attention of a wide range of researchers in chromatography.

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Acknowledgements

This work was supported by a Grant-in-Aid for Early-Career Scientists (23K13771) from the Japan Society for the Promotion of Science (A.M.).

Funding

Japan Society for the Promotion of Science,No. 23K13771,Akihisa Miyagawa

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Pure and Applied Sciences, University of Tsukuba, Tsukuba, Ibaraki, 305-8577, Japan

    Akihisa Miyagawa, Kazuki Yamada & Kiyoharu Nakatani

Authors
  1. Akihisa Miyagawa
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  2. Kazuki Yamada
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  3. Kiyoharu Nakatani
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Correspondence to Akihisa Miyagawa.

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Miyagawa, A., Yamada, K. & Nakatani, K. Investigating hydrophobic environment in alkyl-group-functionalized silica particle with various chain lengths using absorption microspectroscopy. ANAL. SCI. 40, 93–99 (2024). https://doi.org/10.1007/s44211-023-00434-1

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  • DOI: https://doi.org/10.1007/s44211-023-00434-1

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