Skip to main content
SpringerLink
Log in

Nanozyme colorimetric sensing of L-cysteine and copper ions based on PtCo nanoparticles@multi-walled carbon nanotubes

  • Original Paper
  • Published:
Analytical Sciences Aims and scope Submit manuscript

Abstract

In this work, PtCo bimetallic nanoparticles supported on multi-walled carbon nanotubes (PtCo@MWCNTs) nanohybrid was prepared simply and used for the first time as a novel nanozyme in the colorimetric sensing of L-cysteine (L-Cys) and Cu2+. Due to its strong enzyme-like catalytic activity, the prepared PtCo@MWCNTs nanohybrid can catalyze the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) to form ox-TMB without H2O2. Interestingly, the oxidase-like active of PtCo@MWCNTs was effectively suppressed by L-Cys, which could reduce ox-TMB to colorless TMB and lead to a pronounced blue fading, and the absorbance at 652 nm gradually decreased with increasing L-Cys concentration. On the other hand, the nanozyme activity of PtCo@MWCNTs can be recovered due to the complexation between L-Cys and Cu2+. Therefore, a colorimetric method based on PtCo@MWCNTs nanozyme was established to detect L-Cys and Cu2+. The results show that the assay platform has simple, rapid, sensitive performance and good selectivity. The detection limits for L-Cys and Cu2+ are 0.041 μM and 0.056 μM, respectively, coupled with the linearities of 0.01 ~ 60.0 μM and 0.05 ~ 80.0 μM. The successful first application of PtCo bimetal-based nanozyme in colorimetric sensing herein opens a new direction for nanozyme and colorimetric analysis, showing great potential applications.

Graphical Abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Scheme 1
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

Data availability

All of the data generated or analyzed during this study are included in this published article and its supplementary materials.

References

  1. M. Yang, J. Fan, W. Sun, J. Du, X. Peng, Anal. Chem. 91, 12531 (2019)

    Article  CAS  PubMed  Google Scholar 

  2. H. Wu, J. Liu, Z. Chen, P. Lin, W. Ou, Z. Wang, W. Xiao, Y. Chen, D. Cao, Langmuir 38, 8266 (2022)

    Article  CAS  PubMed  Google Scholar 

  3. S. Ruan, Y. Zhou, M. Zhang, H. Zhang, Y. Wang, P. Hu, Anal. Sci. 38, 541 (2022)

    Article  CAS  PubMed  Google Scholar 

  4. S. Hashmi, M. Singh, P. Weerathunge, E.L.H. Mayes, P.D. Mariathomas, S.N. Prasad, R. Ramanathan, V. Bansal, A.C.S. Appl, Nano Mater. 4, 13352 (2021)

    CAS  Google Scholar 

  5. S. Feng, F. Wen, L. He, J. Su, P. Jiang, D. He, Sens. Actuat. B: Chem. l 361, 131745 (2022)

    Article  CAS  Google Scholar 

  6. S. Chen, M. Chi, Y. Zhu, M. Gao, C. Wang, X. Lu, Appl. Surf. Sci. 440, 237 (2018)

    Article  CAS  Google Scholar 

  7. M. Chi, Y. Zhu, L. Jing, C. Wang, X. Lu, Anal. Chim. Acta 1035, 146 (2018)

    Article  CAS  PubMed  Google Scholar 

  8. M. Gao, X. Lu, S. Chen, D. Tian, Y. Zhu, C. Wang, A.C.S. Appl, Nano Mater. 1, 4703 (2018)

    CAS  Google Scholar 

  9. Z. Yu, C. Hu, L. Guan, W. Zhang, J. Gu, A.C.S. Sustain, Chem. Eng. 8, 12713 (2020)

    CAS  Google Scholar 

  10. M.K. Okla, S. Kokilavani, A. Mohebaldin, A.M. Thomas, W. Soufan, M.A. Abdel-Maksoud, H. AbdElgawad, L.L. Raju, S.S. Khan, Colloid. Surf. A. 640, 128318 (2022)

    Article  CAS  Google Scholar 

  11. L. Wan, L. Wu, S. Su, D. Zhu, J. Chao, L. Wang, Chem. Commun. 56, 12351 (2020)

    Article  CAS  Google Scholar 

  12. M. Singh, P. Weerathunge, P.D. Liyanage, E. Mayes, R. Ramanathan, V. Bansal, Langmuir 33, 10006 (2017)

    Article  CAS  PubMed  Google Scholar 

  13. D. Zhu, X. Yan, A. Ren, W. Xie, Z. Duan, Anal. Chim. Acta 1058, 136 (2019)

    Article  CAS  PubMed  Google Scholar 

  14. Z. Li, J.R. Askim, K.S. Suslick, Chem. Rev. 119, 231 (2018)

    Article  PubMed  Google Scholar 

  15. C. Chen, Y. Wang, D. Zhang, Microchim. Acta 186, 784 (2019)

    Article  CAS  Google Scholar 

  16. M. Sabela, S. Balme, M. Bechelany, J.-M. Janot, K. Bisetty, Adv. Eng. Mater. 19, 1700270 (2017)

    Article  Google Scholar 

  17. Z. Xian, L. Zhang, Y. Yu, B. Lin, Y. Wang, M. Guo, Y. Cao, Microchim. Acta 188, 65 (2021)

    Article  CAS  Google Scholar 

  18. M. Xu, X. Li, J.Q. Sha, Z. Tong, Q. Li, C. Liu, Chemistry 27, 9141 (2021)

    Article  CAS  PubMed  Google Scholar 

  19. S.K. Kim, M. Gupta, H.-I. Lee, Sens. Actuat. B: Chem. 257, 728 (2018)

    Article  CAS  Google Scholar 

  20. Z. Xu, Q. Meng, Q. Cao, Y. Xiao, H. Liu, G. Han, S. Wei, J. Yan, L. Wu, Anal. Chem. 92, 2201 (2019)

    Article  Google Scholar 

  21. C. Wang, X. Bi, M. Wang, X. Zhao, Y. Lin, Anal. Chem. 91, 16010 (2019)

    Article  CAS  PubMed  Google Scholar 

  22. Y. Yi, Y. Ma, F. Ai, Y. Xia, H. Lin, G. Zhu, Chem. Commun. 57, 7790 (2021)

    Article  CAS  Google Scholar 

  23. Y. Feng, Y. Yang, Y. Wang, F. Qiu, X. Song, X. Tang, G. Zhang, W. Liu, Sensors Actuat. B: Chem. 288, 27 (2019)

    Article  CAS  Google Scholar 

  24. L. Wang, Z.-L. Wei, Z.-Z. Chen, C. Liu, W.-K. Dong, Y.-J. Ding, Microchem. Journal 155, 104801 (2020)

    Article  CAS  Google Scholar 

  25. Y. Xia, Y. Zhao, F. Ai, Y. Yi, T. Liu, H. Lin, G. Zhu, J. Hazard. Mater. 425, 127974 (2022)

    Article  CAS  PubMed  Google Scholar 

  26. S. Zheng, D. Li, E.K. Fodjo, W. Deng, Chem. Eng. J. 399, 125840 (2020)

    Article  CAS  Google Scholar 

  27. R. Kaushik, R. Sakla, N. Kumar, A. Ghosh, V.D. Ghule, D.A. Jose, Sensors Actuat. B: Chem. 328, 129026 (2021)

    Article  CAS  Google Scholar 

  28. P. Wang, L. Sun, J. Wu, X. Yang, P. Lin, M. Wang, J. Hazard. Mater. 407, 124388 (2021)

    Article  CAS  PubMed  Google Scholar 

  29. Y. Xia, Y. Ma, Y. Wu, Y. Yi, H. Lin, G. Zhu, Microchim. Acta 188, 1 (2021)

    Article  Google Scholar 

  30. L. Wang, M. Li, W. Li, Y. Han, Y. Liu, Z. Li, B. Zhang, D. Pan, A.C.S. Sustain, Chem. Eng. 6, 12668 (2018)

    CAS  Google Scholar 

  31. D. Jiang, D. Ni, Z.T. Rosenkrans, P. Huang, X. Yan, W. Cai, Chemi. Soc. Rev. 48, 3683 (2019)

    Article  CAS  Google Scholar 

  32. L. Zhang, G.-P. Yang, S.-J. Xiao, Q.-G. Tan, Q.-Q. Zheng, R.-P. Liang, J.-D. Qiu, Small 17, 2102944 (2021)

    Article  CAS  Google Scholar 

  33. S. Li, Z. Zhou, Z. Tie, B. Wang, M. Ye, L. Du, R. Cui, W. Liu, C. Wan, Q. Liu, Nat. Commun. 13, 1 (2022)

    Google Scholar 

  34. X. Liang, L. Han, Adv. Func. Mater. 30, 2001933 (2020)

    Article  CAS  Google Scholar 

  35. P. Weerathunge, R. Ramanathan, V.A. Torok, K. Hodgson, Y. Xu, R. Goodacre, B.K. Behera, V. Bansal, Anal. Chem. 91, 3270 (2019)

    Article  CAS  PubMed  Google Scholar 

  36. D. Huang, Y. Wu, F. Ai, X. Zhou, G. Zhu, Sens. Actuat. B: Chem. 342, 130074 (2021)

    Article  CAS  Google Scholar 

  37. S. Cai, C. Qi, Y. Li, Q. Han, R. Yang, C. Wang, J Mater Chem B 4, 1869 (2016)

    Article  CAS  PubMed  Google Scholar 

  38. D. Pedone, M. Moglianetti, M. Lettieri, G. Marrazza, P.P. Pompa, Anal. Chem. 92, 8660 (2020)

    Article  CAS  PubMed  Google Scholar 

  39. L. Huang, D.-W. Sun, H. Pu, Q. Wei, L. Luo, J. Wang, Sens. Actuat. B: Chem. 290, 573 (2019)

    Article  CAS  Google Scholar 

  40. Z. Lu, N. Lu, Y. Xiao, Y. Zhang, Z. Tang, M. Zhang, A.C.S. Appl, Mater. Inter. 14, 11156 (2022)

    Article  CAS  Google Scholar 

  41. W. Duan, Z. Qiu, S. Cao, Q. Guo, J. Huang, J. Xing, X. Lu, J. Zeng, Biosens. Bioelectron. 196, 113724 (2022)

    Article  CAS  PubMed  Google Scholar 

  42. S.-H. Wen, X.-L. Zhong, Y.-D. Wu, R.-P. Liang, L. Zhang, J.-D. Qiu, Anal. Chem. 91, 6487 (2019)

    Article  CAS  PubMed  Google Scholar 

  43. S. Chen, Z. Li, Z. Huang, Q. Jia, S Sens. Actuat. B: Chem. 332, 129522 (2021)

    Article  CAS  Google Scholar 

  44. T. Chen, J. Cao, X. Bao, Y. Peng, L. Liu, W. Fu, RSC Adv. 11, 39966 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  45. Z. Qiu, W. Duan, S. Cao, T. Zeng, T. Zhao, J. Huang, X. Lu, J. Zeng, Environ. Sci. Technol. 56, 1713 (2022)

    Article  CAS  PubMed  Google Scholar 

  46. H. Chen, C. Yuan, X. Yang, X. Cheng, A.A. Elzatahry, A. Alghamdi, J. Su, X. He, Y. Deng, A.C.S. Appl, Nano Mater. 3, 4586 (2020)

    CAS  Google Scholar 

  47. E. Boselli, Z. Wu, A. Friedman, B. Claus Henn, I. Papautsky, E. Environ, Sci. Technol. 55, 7501 (2021)

    Article  CAS  Google Scholar 

  48. Y. Chen, P. Wang, H. Hao, J. Hong, H. Li, S. Ji, A. Li, R. Gao, J. Dong, X. Han, M. Liang, D. Wang, Y. Li, J. Am. Chem. Soc. 143, 18643 (2021)

    Article  CAS  PubMed  Google Scholar 

  49. S. Bhattacharyya, S.R. Ali, M. Venkateswarulu, P. Howlader, E. Zangrando, M. De, P.S. Mukherjee, J. Am. Chem. Soc. 142, 18981 (2020)

    Article  CAS  PubMed  Google Scholar 

  50. M. Wang, X. Zhou, S. Wang, X. Xie, Y. Wang, X. Su, Anal. Chem. 93, 3130 (2021)

    Article  CAS  PubMed  Google Scholar 

  51. Y. Xia, J. Li, G. Zhu, Y. Yi, Sensors Actuat. B: Chem. 355, 131247 (2022)

    Article  CAS  Google Scholar 

  52. Y. Zhao, Y. Xia, J. Zhang, H. Liu, Y. Yi, G. Zhu, Microchem. J. 185, 108207 (2023)

    Article  CAS  Google Scholar 

  53. S. Kavitha, S.M.J. Kala, A.A.B. Christus, A. Ravikumar, RSC Adv. 11, 37162 (2021)

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  54. X. Zhou, C. Fan, Q. Tian, C. Han, Z. Yin, Z. Dong, S. Bi, Anal. Chem. 94, 847 (2021)

    Article  PubMed  Google Scholar 

  55. Z. Zhu, Q. Xu, Z. Ni, K. Luo, Y. Liu, D. Yuan, A.C.S. Sustain, Chem. Eng. 9, 13491 (2021)

    CAS  Google Scholar 

  56. J. Qi, L. Xin, Z. Zhang, K. Sun, H. He, F. Wang, D. Chadderdon, Y. Qiu, C. Liang, W. Li, Green Chem. 15, 1133 (2013)

    Article  CAS  Google Scholar 

  57. S.-W. Lv, J.-M. Liu, N. Zhao, C.-Y. Li, F.-E. Yang, Z.-H. Wang, S. Wang, Sep. Purif. Technol. 253, 117413 (2020)

    Article  CAS  Google Scholar 

  58. Y. Zeng, Y. Li, X. Tan, J. Gong, Z. Wang, Y. An, Z. Wang, H. Li, A.C.S. Appl, Mater. Inter. 13, 36816 (2021)

    Article  CAS  Google Scholar 

  59. X. Wei, J. Chen, M.C. Ali, J.C. Munyemana, H. Qiu, Microchim. Acta 187, 1 (2020)

    Article  Google Scholar 

  60. X. Zheng, Z. Liu, Q. Lian, H. Liu, L. Chen, L. Zhou, Y. Jiang, J. Gao, A.C.S. Sustain, Chem. Eng. 9, 12766 (2021)

    CAS  Google Scholar 

  61. S. Cai, X. Jia, Q. Han, X. Yan, R. Yang, C. Wang, Nano Res. 10, 2056 (2017)

    Article  CAS  Google Scholar 

  62. X. Li, X.-Y. Yang, J.-Q. Sha, T. Han, C.-J. Du, Y.-J. Sun, Y.-Q. Lan, A.C.S. Appl, Mater. Inter. 11, 16896 (2019)

    Article  CAS  Google Scholar 

  63. L. Gao, J. Zhuang, L. Nie, J. Zhang, Y. Zhang, N. Gu, T. Wang, J. Feng, D. Yang, S. Perrett, X. Yan, Nat. Nanotechnol. 2, 577 (2007)

    Article  CAS  PubMed  Google Scholar 

  64. J. Dong, L. Song, J.-J. Yin, W. He, Y. Wu, N. Gu, Y. Zhang, A.C.S. Appl, Mater. Inter. 6, 1959 (2014)

    Article  CAS  Google Scholar 

  65. D. Jampaiah, T. Srinivasa Reddy, V.E. Coyle, A. Nafady, S.K. Bhargava, J. Mater. Chem. B 5, 720 (2017)

    Article  CAS  PubMed  Google Scholar 

  66. M. Moglianetti, E. De Luca, D. Pedone, R. Marotta, T. Catelani, B. Sartori, H. Amenitsch, S.F. Retta, P.P. Pompa, Nanoscale 8, 3739 (2016)

    Article  CAS  PubMed  Google Scholar 

  67. C. Jin, J. Lian, Y. Gao, K. Guo, K. Wu, L. Gao, X. Zhang, X. Zhang, Q. Liu, A.C.S. Sustain, Chem. Eng. 7, 13989 (2019)

    CAS  Google Scholar 

Download references

Acknowledgements

The work was financed by the Natural Science Foundation of Fujian Province (2019Y0013).

Funding

E Natural Science Foundation of Fujian Province, 2019Y0013, Fanrui Fu.

Author information

Authors and Affiliations

  1. Department of Clinical Laboratory, The Second Affiliated Hospital of Fujian Medical University, Quanzhou, People’s Republic of China

    Xiaorong Lin, Liuxia You & Qiaoling Liu

  2. Department of General Surgery, the Second Affiliated Hospital of Fujian Medical University, Quanzhou, People’s Republic of China

    Changting Wang

  3. West China Xiamen Hospital of Sichuan University, Xiamen, People’s Republic of China

    Fanrui Fu

Authors
  1. Xiaorong Lin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Changting Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Liuxia You
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Fanrui Fu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Qiaoling Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Fanrui Fu or Qiaoling Liu.

Ethics declarations

Conflict of interest

On behalf of all authors, the corresponding author states that there is no conflict of interest.

Supplementary Information

Below is the link to the electronic supplementary material.

Supplementary file1 (DOCX 254 KB)

Rights and permissions

Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Lin, X., Wang, C., You, L. et al. Nanozyme colorimetric sensing of L-cysteine and copper ions based on PtCo nanoparticles@multi-walled carbon nanotubes. ANAL. SCI. 39, 1669–1679 (2023). https://doi.org/10.1007/s44211-023-00411-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s44211-023-00411-8

Keywords

Search

Navigation