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Profiling Prokaryotic Communities and Aaptamines of Sponge Aaptos suberitoides from Tulamben, Bali

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Abstract

Sponges (Porifera) harbor a diversity of microorganisms that contribute largely to the production a vast array of bioactive compounds. The microorganisms associated with sponge have an important impact on the chemical diversity of the natural products. Herein, our study focuses on an Aaptos suberitoides commonly found in Indonesia. The objective of this study was to investigate the profile of prokaryotic community and the presence of aaptamine metabolites in sponge Aaptos suberitoides. Sponges were collected from two site locations (Liberty Wreck and Drop Off) in Tulamben, Bali. The sponges were identified by barcoding DNA cytochrome oxidase subunit I (COI) gene. The profile of prokaryotic composition was investigated by amplifying the 16S rRNA gene using primers 515f and 806r to target the V4 region. The metabolites were analyzed using LC-MS, and dereplication was done to identify the aaptamines and its derivates. The barcoding DNA of the sponges confirmed the identity of samples as Aaptos suberitoides. The prokaryotic communities of samples A. suberitoides were enriched and dominated by taxa Proteobacteria, Chloroflexi, Actinobacteria, and Acidobacteria. The chemical analysis showed that all sponges produce aaptamine and isoaaptamine except A. suberitoides S2421 produce analog of aaptamines. This is the first report on the profile of prokaryotic community and the aaptamine of tropical marine sponges, A. suberitoides, from Tulamben, Bali.

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Acknowledgements

The authors would like to thank Nenik Kholilah, Fanny Aljihad, Nining Nursalim, and Amelia Cahya Anggelina for helping the field collection.

Funding

This project was funded by Universitas Gadjah Mada Hibah Post Doc 2022–2023 batch 2 contract no 15734/IT3/ TU.00.01/ M/B/2021.

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Authors and Affiliations

  1. Biology Department, Faculty of Science and Mathematics, Diponegoro University, Semarang, Central Java, Indonesia

    Ni Kadek Dita Cahyani

  2. Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Noer Kasanah

  3. Department of Biotechnology, Graduate School, Universitas Gadjah Mada, Yogyakarta, Indonesia

    Dewi Sri Kurnia

  4. Department of Drug Discovery and Biomedical Sciences, College of Pharmacy, Medical University of South Carolina, Charleston, SC, USA

    Mark T. Hamann

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  1. Ni Kadek Dita Cahyani
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  2. Noer Kasanah
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Contributions

Ni Kadek Dita Cahyani: propose a study, collected samples, analyzed DNA data of microbiome, wrote and revised the draft manuscript, grant funding. Noer Kasanah: concept the study, collected samples, analyzed mass spectra data, supervised, wrote and reviewed the manuscript, grant funding. Dewi Sri Kurnia: analyzed spectra data, dereplication and supplemented spectra data. Mark T. Hamann: supervised the research, reviewed and revised the manuscript, grant funding.

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Correspondence to Noer Kasanah.

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Cahyani, N.K.D., Kasanah, N., Kurnia, D.S. et al. Profiling Prokaryotic Communities and Aaptamines of Sponge Aaptos suberitoides from Tulamben, Bali. Mar Biotechnol 25, 1158–1175 (2023). https://doi.org/10.1007/s10126-023-10268-7

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