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Chemical characterization of the crude extract of Sauromatum venosum (voodoo lily) and docking study with 12-O-acetylingol 8-tiglate for cytotoxicity testing in SaOS2 (osteoblastic osteosarcoma cells)

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Abstract

The current study was carried out to investigate the anticancer potential of Sauromatum venosum (SV) tuber by gas chromatography with high-resolution mass spectrometry (GC–HRMS) analysis of ethanolic (eSV), hydroalcoholic (hSV), and aqueous extracts (wSV), and in silico study were performed to investigate the main targets of 12-O-acetylingol 8-tiglate by computational docking. The GC–HRMS analysis of three plant samples was carried out on a system equipped with a high-resolution mass spectrometer. The major compounds were identified in all crude extracts. Computation docking analysis was performed for the prediction of the main target of the cancer proliferation of active compound of the Sauromatum venosum tuber extract in cancer therapy. A total of 45 phytocompounds were detected including diterpenoids, esters of fatty acid, hydrocarbons, and alkanes in the tuber of SV. Among all the crude samples tested, eSV showed the lowest IC50 value treated with SaOS2 cells. 12-O-acetylingol 8-tiglate is one of the phytocompounds identified in eSV extract and has been found to exhibit cytotoxic effects against various cancer cells, as reported in the research. It shows the optimum binding affinity with − 8.59 kcal/mol binding energy with a molecular target protein TNF-α (PDB ID: 7PKA). The observed interactions strongly support the anticancer activity of 12-O-acetylingol 8-tiglate and its role in the medicinal efficacy of the plant. These findings highlight the potential of the compound as a valuable source for the development of a therapeutic agent aimed at combating cancer. However, it is important to note that additional in vitro and in vivo studies are required to validate these findings and establish the therapeutic potential.

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Data availability

The supportive data of the findings of this study are available from the co-responding author upon the reasonable request.

Abbreviations

GC–HRMS:

Gas chromatograph–high-resolution mass spectrometer

TLC:

Thin-layer chromatography

UV–Vis:

Ultra violet–visible

WHO:

World Health Organization

SV:

Sauromatum venosum

eSV:

Ethanolic crude extract

hSV:

Hydroalcoholic crude extract

wSV:

Aqueous crude extract

TNF- α:

Tumor necrosis factor-alpha

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Acknowledgements

Thanks to the Guru Ghasidas Vishwavidyalaya and Central Council for Research in Ayurveda Science (CCRAS), New Delhi, for supporting the research work, Mr. Puran Chabbaria of Aarpaa Sone Paryavaran Samiti, Pendra, (C.G.) for providing plant tuber samples, Sophisticated Analysis Facility (SAIF), IIT Bombay, for carrying out GC–HRMS analysis.

Funding

Guru Ghasidas Central Vishwavidyalaya (GGV) Bilaspur (C.G.), Central Council for Research in Ayurvedic Science (CCRAS), New Delhi.

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Authors and Affiliations

  1. Department of Zoology, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh, 495009, India

    Namrata Kahar & Rohit Seth

  2. Sickle Cell Institute, Chhattisgarh, Raipur, India

    Preeti Mishra

  3. Department of Biotechnology, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh, 495009, India

    Renu Bhatt

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  1. Namrata Kahar
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NK: conceptualization, methodology, resources, investigation, and writing—manuscript. PM: in silico study, Investigation. Dr. RS: writing—reviewing and editing, supervision, approval of the final version of the manuscript. Dr. RB: supervision.

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Correspondence to Rohit Seth.

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Kahar, N., Mishra, P., Bhatt, R. et al. Chemical characterization of the crude extract of Sauromatum venosum (voodoo lily) and docking study with 12-O-acetylingol 8-tiglate for cytotoxicity testing in SaOS2 (osteoblastic osteosarcoma cells). ANAL. SCI. 40, 151–162 (2024). https://doi.org/10.1007/s44211-023-00441-2

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