Abstract
This study focuses on the analyses of the gastral cavity contents of two species of Ceriantharia, namely Isarachnanthus nocturnus Hartog, 1977, collected in São Sebastião, Brazil, and Pachycerianthus magnus Nakamoto, 1919, collected from two points along the coast of Okinawa Island, Japan. Both morphological (light microscopy) and metagenomic (whole-genome shotgun sequencing) analyses were conducted with the aim of identifying the main classes of organisms that make up the diet of these species. The results revealed that morphological analyses were not sufficient for identifying prey organisms, but metagenomic analyses showed a diverse range of animal classes, indicating the varied diet of these ceriantharians. The most abundant classes observed in the contents of both species were Anthozoa, Aconoidasida, Kinetoplastea, Actinopterygii, Insecta, and Leptocardii. This finding suggests a cohesive feeding pattern among these two species from distant areas, providing a more comprehensive understanding of their feeding performance and contributing to studies on the subclass Ceriantharia.
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All genomic data is available via Genbank.
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Acknowledgements
This study was supported by São Paulo Research Foundation (FAPESP) [Proc. n. 2018/07622-0, 2019/14236-2, 2019/03552-0 and 2022/16193-1]. SNS was supported by National Council of Scientific and Technological Development (CNPq -Research Productivity Scholarship) grant number 301293/2019-8 and 304267/2022-8. We are grateful to all the MISE laboratory members for providing laboratory and technical support and to Dr. André C. Morandini (IB-USP) who provided constructive comments on the early version of the manuscript
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Santos, T.B., Poliseno, A., Bendia, A.G. et al. Unlocking the jar: revealing gastric content in Ceriantharia (Cnidaria, Anthozoa) through whole-genome shotgun sequencing. Aquat Ecol (2023). https://doi.org/10.1007/s10452-023-10076-0
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DOI: https://doi.org/10.1007/s10452-023-10076-0