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Formulation and Characterization of Luliconazole Microsponge Gel for Diaper Dermatitis

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Abstract

Purpose

Many current drug delivery technologies are ineffective and require high concentrations of active drugs for effective therapy. Novel drug delivery systems (NDDS) are increasingly being investigated to achieve targeted and controlled drug release. This study uses a microsponge-loaded drug delivery system to overcome the problem associated with traditional therapy for diaper dermatitis. Therefore, we aimed to formulate and characterize luliconazole microsponge-loaded gel for anti-fungal activity.

Methods

Quasi-emulsion solvent diffusion method was used for developing luliconazole microsponge. The characterization of drug-loaded microsponge was investigated. Optimization of formulation carried out through in vitro release studies and entrapment efficacy. The formulation of microsponge-loaded gel was carried out and evaluated using physicochemical studies. The effectiveness of optimized formulations was examined for anti-fungal activity against Candida albicans.

Results

The prepared microsponge was found to have a particle size between 20 and 50 µm, an entrapment efficiency of 53 ± 0.3–92.7 ± 0.1% and a drug release of 71.8 ± 0.2–88.5 ± 0.02%. The pH was in the range of 6.8–7.4, and the viscosity of the prepared formulation was 265.5 cPs. In vitro, the release of luliconazole microsponge-loaded gel showed that formulation F6 has the highest percentage (%) of cumulative drug release (CDR) compared to other formulations. The anti-fungal activity of the microsponge-loaded gel against Candida albicans showed a clear zone of inhibition in the F6 formulation.

Conclusion

Our study results demonstrate that the F6 formulation has the highest % of CDR and shows better anti-fungal activity. Therefore, luliconazole microsponge-loaded gel formulation can improve drug delivery in diaper rash caused by fungal infection.

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Data Availability

The data obtained in this study can be availed upon request.

References

  1. Bhanu PV, Shanmugam V, Lakshmi PK. Development and optimization of novel diclofenac emulgel for topical drug delivery. Int J Compr Pharm. 2011;2:1–4.

    Google Scholar 

  2. Jadhav N, Patel V, Mungekar S, Bhamare GKM. Microsponge delivery system: an updated review, current status and future prospects. J Sci Innov Res. 2013;2:1097–110.

    Google Scholar 

  3. Mani S. A current view on microsponge drug delivery system. Eur J Mol Biol Biochem. 2016;3:33–8.

    Google Scholar 

  4. Devi N, Kumar S, Prasad M, Rao R. Eudragit RS100 based microsponges for dermal delivery of clobetasol propionate in psoriasis management. J Drug Deliv Sci Technol. 2020;55: 101347.

    Article  CAS  Google Scholar 

  5. Mandava SS, Thavva V. Novel approach: microsponge drug delivery system. Int J Pharm Sci Res. 2012;3:967–80.

    CAS  Google Scholar 

  6. Tile MK, Pawar AY. Microsponges: a novel strategy for drug delivery. Int J Pure Appl Biosci. 2015;3:224–35.

    Google Scholar 

  7. Kumari A, Jain A, Hurkat P, Verma A, Jain SK. Microsponges: A pioneering tool for biomedical applications. Crit Rev TherDrug Carrier Syst. 2016;33:77–105.

    Article  Google Scholar 

  8. Aloorkar NH, Kulkarni AS, Ingale DJ, Patil RA. Microsponges as innovative drug delivery systems. Int J Pharm SciNanotechnol. 2012;5:1597–606.

    Google Scholar 

  9. Patil RS, Kemkar VU, Patil SS. Microsponge drug delivery system: a novel dosage form. Am J PharmTech Res. 2012;2:227–51.

    Google Scholar 

  10. Harish NM, Prabhu P, Charyulu RN, Gulzar MA, Subrahmanyam EV. Formulation and evaluation of in situ gels containingclotrimazole for oral candidiasis. Indian J Pharm Sci. 2009;71:421–7.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Mahant S, Kumar S, Nanda S, Rao R. Microsponges for dermatological applications: perspectives and challenges. Asian JPharm Sci. 2020;15:273–91.

    Google Scholar 

  12. Biharee A, Bhartiya S, Yadav A, Thareja S, Jain AK. Microsponges as drug delivery system: past, present, and futureperspectives. Curr Pharm Des. 2023;29:1026–45.

    Article  CAS  PubMed  Google Scholar 

  13. Jayasawal P, Rao NR, Jakhmola V. Microsponge as novel drug delivery system: a review. Indo Glob J Pharm Sci. 2022;12:21–9.

    Article  Google Scholar 

  14. Singhvi G, Manchanda P, Hans N, Dubey SK, Gupta G. Microsponge: an emerging drug delivery strategy. Drug Dev Res. 2019;80:200–8.

    Article  CAS  PubMed  Google Scholar 

  15. Sammour RMF, Khan G, Sameer S, Khan S, Zohair T, Saraya S, Abdul Rasool BK. Development of clindamycin loaded oralmicrosponges (Clindasponges) for antimicrobial enhancement: in vitro characterization and simulated in vivo studies. Biol PharmBull. 2023;46:1088–97.

    CAS  Google Scholar 

  16. Jain N, Sharma PK, Banik A. Recent advances on microsponge delivery system. Int J Pharm Sci Rev Res. 2011;8:13–23.

    CAS  Google Scholar 

  17. Ravanfar P, Wallace JS, Pace NC. Diaper dermatitis: a review and update. Curr Opin Pediatr. 2012;24:472–9.

    Article  PubMed  Google Scholar 

  18. Prasad HRY, Srivastava P, Verma KK. Diaper dermatitis - an overview. Indian J Pediatr. 2003;70:635–7.

    Article  CAS  PubMed  Google Scholar 

  19. Vyas LK, Tapar KK, Laddha BH, Lahoti AO, Nema RK. formulation and development of anti blemish preparation usingmicrosponge technology. J Chem Pharm Res. 2010;2:562–71.

    CAS  Google Scholar 

  20. Kaity S, Maiti S, Ghosh AK, Pal D, Ghosh A, Banerjee S. Microsponges: a novel strategy for drug delivery system. J AdvPharm Technol Res. 2010;1:283–90.

  21. Pande VV, Kadnor NA, Kadam RN, Upadhye SA. Fabrication and characterization of sertaconazole nitrate microsponge as atopical drug delivery system. Indian J Pharm Sci. 2015;77:675–80.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Nocent M, Bertocchi L, Espitalier F, Baron M, Couarraze G. Definitin of a solvent system for spherical crystallization ofsalbutamol sulfate by quasi-emulsion solvent diffusion (QESD) method. J Pharm Sci. 2001;90:1620–7.

    Article  CAS  PubMed  Google Scholar 

  23. Booravilli J, Sirisolla JD, Saluru S. Formulation and evaluation of ketoconazole microsponge topical gel. Int J Res Pharm Sci. 2022;13:382–91.

    Article  CAS  Google Scholar 

  24. Kumar PM, Ghosh A. Development and evaluation of silver sulfadiazine loaded microsponge based gel for partial thickness(second degree) burn wounds. Eur J Pharm Sci. 2017;96:243–54.

    Article  CAS  PubMed  Google Scholar 

  25. Pradhan SK. Microsponges as the versatile tool for drug delivery system. Int J Res Pharm Chem. 2011;1:243–58.

    CAS  Google Scholar 

  26. Taghipour S, Kiasat N, Shafiei S, Halvaeezadeh M, Rezaei-Matehkolaei A, Zarei MA. Luliconazole, a new antifungal against Candida species isolated from different sources. J Mycol Med. 2018;28:374–8.

    Article  CAS  PubMed  Google Scholar 

  27. Koga H, Nanjoh Y, Makimura K, Tsuboi R. In vitro antifungal activities of luliconazole, a new topical imidazole. MedMycol. 2009;47:640–7.

    CAS  Google Scholar 

  28. Ubaid M, Ilyas S, Mir S, Khan AK, Rashid R, Khan MZ, Kanwal ZG, Nawaz A, Shah A, Murtaza G. Formulation and invitro evaluation of carbopol 934-based modified clotrimazole gel for topical application. An Acad Bras Cienc. 2016;88:2303–17.

    Article  CAS  PubMed  Google Scholar 

  29. Parashar B, Kabra A, Chandel A. Formulation and evaluation of gel containing miconazole nitrate an antifungal agent. Int JPharm Res Rev. 2013;2:18–28.

    CAS  Google Scholar 

  30. Comoglu T, Gonul N, Baykara T. Preparation and in vitro evaluation of modified release ketoprofen microsponges. IlFarmaco. 2003;58:101–6.

  31. Perumal D. Microencapsulation of ibuprofen and eudragit RS 100 by emusion solvent diffusion method. Int J Pharm. 2001;218:1–11.

    Article  CAS  PubMed  Google Scholar 

  32. Sadeghi-Ghadi Z, Behjou N, Ebrahimnejad P, Mahkam M, Goli HR, Lam M, Nokhodchi A. Improving antibacterialefficiency of Curcumin in magnetic polymeric nanocomposites. J Pharm Innov Springer. 2023;18:13–28.

    Article  Google Scholar 

  33. Jelvehgari M, Siahi-shadbad MR, Azarmi S, Martin GPNA. The microsponge delivery system of benzoyl peroxide:preparation, characterization and release studies. Int J Pharm. 2006;308:124–32.

    Article  CAS  PubMed  Google Scholar 

  34. Khanna D, Bharti S. Luliconazole for the treatment of fungal infections: an evidence-based review. Core Evid. 2014;9:113–24.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Shankar D, Gajanan S, Suresh J, Dushyant G. Formulation and evaluation of luliconazole emulgel for topical drug delivery. Int Res J Sci Eng. 2018;3:85–9.

  36. Orlu M, Cevher E, Araman A. Design and evaluation of colon specific drug delivery system containing flurbiprofenmicrosponges. Int J Pharm. 2006;318:103–17.

    Article  CAS  PubMed  Google Scholar 

  37. Sato T, Kanke M, Schroeder HG, DeLuca PP. Porous biodegradable microspheres for controlled drug delivery. I. Assessment of processing conditions and solvent removal techniques. Pharm Res. 1988; 5:21–30.

  38. Wakiyama N, Juni K, Nakano M. Preparation and evaluation in vitro of polylactic acid microspheres containing localanesthetics. Chem Pharm Bull (Tokyo). 1981;29:3363–8.

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgements

The authors are grateful to the Sri Ramachandra Institute of Higher Education and Research (SRIHER), Porur, Chennai, for the opportunity to conduct the research.

Funding

There was no specific grant for this research from public, private, or nonprofit funding organisations.

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Author notes

  1. Pavithra Bharathy

    Present address: Department of Pharmaceutics, Saveetha College of Pharmacy, Saveetha University, Chennai, Tamil Nadu, 602105, India

Authors and Affiliations

  1. Department of Pharmaceutical Chemistry, Saveetha College of Pharmacy, Saveetha University, Chennai, Tamil Nadu, 602105, India

    Punniyakoti V. Thanikachalam

  2. Sri Ramachandra Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research, Porur, Chennai, Tamil Nadu, India

    Nithya Priya Parthasarathy, Pavithra Elumalai & Pavithra Krishnamoorthy Baskaran

  3. Crescent School of Pharmacy, B. S. Abdur Rahman Crescent Institute of Science and Technology, Vandalur, Chennai, Tamil Nadu, India

    Thameemul Ansari L. H.

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Contributions

TALH: conceptualization, validation, visualization, and supervision; PB, NPP, PE, and PKB: made substantial contributions to the acquisition, analysis, or interpretation of data; PVT: conceptualization, drafted the work and revised it critically for important intellectual content. All authors approved the version to be published and agreed to be accountable for all aspects of the work, ensuring that questions related to the accuracy or integrity of any part of the work are appropriately investigated and resolved.

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Correspondence to Punniyakoti V. Thanikachalam or Thameemul Ansari L. H..

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Bharathy, P., Thanikachalam, P.V., Parthasarathy, N.P. et al. Formulation and Characterization of Luliconazole Microsponge Gel for Diaper Dermatitis. J Pharm Innov 18, 2362–2372 (2023). https://doi.org/10.1007/s12247-023-09797-4

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