Abstract
The Chinese softshell turtle (Pelodiscus sinensis) is a kind of subaquatic reptile with high nutritional value, medicinal value and economic value. However, the number of wild turtles has dropped dramatically due to excessive fishing and habitat destruction. Artificial breeding of the turtle is facing the dilemma of germplasm degradation and lack of seedlings, which seriously hinders the sustainable development of the turtle industry. In the present study, 60 novel single nucleotide polymorphism (SNP) markers were developed based on high-throughput sequencing. Genetic diversity analysis revealed that the values of observed heterozygosity (Ho) and expected heterozygosity (He) varied from 0.0312 to 0.7500 and from 0.0312 to 0.4841, respectively, and the minor allele frequency ranged from 0.3333 to 0.5000. Only four loci showed significant deviation from Hardy–Weinberg equilibrium. The present study will provide a valuable tool for population study and resource conservation.
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Acknowledgements
The authors gratefully express appreciation to the efforts and contributions of Shanghai Majorbio Bio-pharm Technology Co., Ltd, China. This work was supported by University Natural Science Research Project of Anhui Province, China (KJ2019ZD59); the Key Research and Development Plan Project of Anhui Province, China (1804a07020119); the Special Fund for Anhui Agriculture Research System, China; the excellent talent foundation of Huainan Normal University, China (GCCRCKYQDJ-xiaomingsong).
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Investigations and protocols were conducted according to the guiding principles for the use and care of laboratory animals and in compliance with Anhui Science and Technology University Institute of Animal Care and Use Committee. The institutional review board approved this procedure. Our study had been submitted to and approved by the Academic Ethics Committee of Anhui Science and Technology University. All sample collection was undertaken in accordance with relevant Academic Ethics Committee of Anhui Science and Technology University guidelines and regulations.
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Xiao, M., Gong, B., Xu, H. et al. Development of 60 SNP markers in Pelodiscus sinensis based on transcriptome sequencing. Conservation Genet Resour 16, 79–83 (2024). https://doi.org/10.1007/s12686-023-01335-1
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DOI: https://doi.org/10.1007/s12686-023-01335-1