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Development of Winter Common Wheat Lines with the Stem Rust Resistance Gene Sr33

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Abstract

Stem rust caused by the fungus Puccinia graminis Pers. is a dangerous disease of wheat that occurs in all regions of its cultivation. New highly virulent races causing severe yield losses have appeared in recent decades. Sr33 introgressed from Aegilops tauschii is one of the genes conferring resistance against most races of stem rust, including Ug99. To develop winter common wheat lines with the gene Sr33 and evaluate a possible effect of the gene on yield traits, the authors made a cross between the spring line DH31 carrying the Sr33 gene and the winter cultivar Myrkhad followed by marker-assisted selection of winter genotypes with Sr33 starting from F3. To identify the Sr33 gene, PCR with the gene-specific marker Sr33A was used. Alleles at the storage protein loci of the parental forms were identified using acid polyacrylamide gel electrophoresis and SDS-electrophoresis as well as the molecular marker MAR for Glu-Blal. As a result of marker-assisted selection, winter F5 lines with the Sr33 gene were developed from the cross DH31 × Myrkhad. The yield traits of F5 spikes of families derived from single F3 spikes with and without Sr33 were analyzed considering that the line DH31 has a specific allele at Gli-D1 from Ae. tauschii, dark glumes, and high molecular weight glutenin subunit alleles associated with high dough strength, in particular, Glu-Blal. Comparison of means of yield traits of spikes from families with Sr33 and without it did not reveal significant differences between these two groups. Thus, winter F5 lines with the Sr33 gene from the cross DH31 × Myrkhad may be used in the breeding practice to develop cultivars with high bread-making qualities of flour and stem rust resistance.

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  • 31 January 2024

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Funding

The work was carried out with the financial support of N.O. Kozub, Y.V. Pirko, I.O. Sozinov, A.V. Karelov, O.I. Sozinova, and A.I. Yemets within the framework of the NFSU project 2021.01/0313 “Creation of Common Wheat Genotypes with Resistance Genes against Highly Pathogenic Stem Rust Strains Using Molecular Markers as a Guarantee of Food Security of Ukraine.”

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Authors and Affiliations

  1. Institute of Food Biotechnology and Genomics, National Academy of Sciences of Ukraine, 04123, Kyiv, Ukraine

    N. O. Kozub, Ya. V. Pirko, A. V. Karelov, O. I Sozinova, B. V. Ivashchuk, A. I. Yemets & Ya. B. Blume

  2. Institute of Plant Protection, National Academy of Agrarian Sciences of Ukraine, 03022, Kyiv, Ukraine

    N. O. Kozub, I. O. Sozinov & O. I Sozinova

  3. Eastern Cereal and Oilseed Research Center, Agriculture and Agrifood Canada, K1A 0C6, Ottawa, Ontario, Canada

    G. Fedak

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  1. N. O. Kozub
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  2. Ya. V. Pirko
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  3. I. O. Sozinov
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  4. A. V. Karelov
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  5. O. I Sozinova
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  6. B. V. Ivashchuk
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Correspondence to N. O. Kozub, Ya. V. Pirko, G. Fedak, A. I. Yemets or Ya. B. Blume.

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Kozub, N.O., Pirko, Y.V., Sozinov, I.O. et al. Development of Winter Common Wheat Lines with the Stem Rust Resistance Gene Sr33. Cytol. Genet. 57, 517–523 (2023). https://doi.org/10.3103/S009545272306004X

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