Abstract
Introduction
Pediatric heart failure imposes a significant health burden, necessitating effective interventions. Left ventricular assist devices (VADs) have emerged as crucial tools for circulatory support in advanced pediatric heart failure cases. However, VAD implantation brings forth the challenge of infections and inflammation, impacting patient outcomes. In this study, we explore the potential of two types pf pharmaceutical formulations, liposomal carriers loaded with gallic acid (GA) and tannic acid (TA) to address these issues.
Methods
Liposomes encapsulating GA and TA were prepared using thin-film hydration. Antimicrobial and antibiofilm efficacy against a dual bacterial system composed of Staphylococcus aureus (S. aureus) and Staphylococcus epidermidis (S. epidermidis) was assessed. The impact on lipopolysaccharide (LPS)-induced human aortic endothelial cells (HAEC) viability, intercellular adhesion molecule 1(ICAM-1) expression, monocyte attachment, and Interleukin 6 (IL-6) production were analyzed.
Results
Both TA- and GA-loaded liposomes demonstrated uniform shape with size around 250 nm. TA-loaded liposomes exhibited superior antibacterial and antibiofilm efficacy against the dual bacteria system compared to GA-loaded liposomes. GA-loaded liposomes significantly improved HAEC viability but TA-liposomes did not substantially enhance cell viability. Both liposomal interventions reduced LPS-induced IL-6 production, ICAM-1 expression, and monocyte attachment on HAECs.
Conclusion
This study highlights the multifaceted potential of GA and TA-liposomes in addressing infections and inflammation associated with pediatric VAD implantation.
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Data Availability
All data generated or analyzed during this study are included in this published article.
References
Jefferies JL, Morales DL. Mechanical circulatory support in children: bridge to transplant versus recovery. Curr Heart Fail Rep. 2012;9(3):236–43.
Amdani S, Bradley SM, Rossano J, et al. Burden of pediatric heart failure in the United States. J Am Coll Cardiol. 2022;79(19):1917–28.
Miller JR, Eghtesady P. Ventricular assist device use in congenital heart disease with a comparison to heart transplant. J Comp Eff Res. 2014;3(5):533–46.
Thompson JH, Faulkner K, Lee C. Adverse events in patients with a left ventricular assist device: are patient-reported outcomes affected? Eur J Cardiovasc Nurs. 2022;21(3):254–60.
Şen S, Ülger Z, Bal ZŞ, Özbaran M. Infections in children with left ventricular assist device. Transplant Infect Dis. 2020;22(6):e13439.
Auerbach SR, Richmond ME, Schumacher KR, et al. Infectious complications of ventricular assist device use in children in the United States: data from the Pediatric Interagency Registry for Mechanical Circulatory Support (Pedimacs). J Heart Lung Transplant. 2018;37(1):46–53.
Cabrera AG, Khan MS, Morales DLS, et al. Infectious complications and outcomes in children supported with left ventricular assist devices. J Heart Lung Transplant. 2013;32(5):518–24.
Toba FA, Akashi H, Arrecubieta C, Lowy FD. Role of biofilm in Staphylococcus aureus and Staphylococcus epidermidis ventricular assist device driveline infections. J Thorac Cardiovasc Surg. 2011;141(5):1259–64.
Burki S, Adachi I. Pediatric ventricular assist devices: current challenges and future prospects. Vasc Health Risk Manag. 2017;13:177–85.
Ragusa R, Prontera C, Di Molfetta A, et al. Time-course of circulating cardiac and inflammatory biomarkers after Ventricular Assist Device implantation: comparison between pediatric and adult patients. Clin Chim Acta. 2018;486:88–93.
Byrnes JW, Bhutta AT, Rettiganti MR, et al. Steroid therapy attenuates acute phase reactant response among children on ventricular assist device support. Ann Thorac Surg. 2015;99(4):1392–8.
Radley G, Pieper IL, Ali S, et al. The inflammatory response to ventricular assist devices. Front Immunol. 2018;9:2651.
Tang PC, Haft JW, Romano MA, et al. Right ventricular failure following left ventricular assist device implantation is associated with a preoperative pro-inflammatory response. J Cardiothorac Surg. 2019;14(1):80.
Patra JK, Das G, Fraceto LF, et al. Nano-based drug delivery systems: recent developments and future prospects. J Nanobiotechnology. 2018;16(1):71.
Ferreira M, Ogren M, Dias JNR, et al. Liposomes as antibiotic delivery systems: a promising nanotechnological strategy against antimicrobial resistance. Molecules. 2021;26(7).
Brusini R, Varna M, Couvreur P. Advanced nanomedicines for the treatment of inflammatory diseases. Adv Drug Deliv Rev. 2020;157:161–78.
Nakhaei P, Margiana R, Bokov DO, et al. Liposomes: structure, biomedical applications, and stability parameters with emphasis on cholesterol. Front Bioeng Biotechnol. 2021;9:705886.
Ferreira M, Pinto SN, Aires-da-Silva F, et al. Liposomes as a nanoplatform to improve the delivery of antibiotics into Staphylococcus aureus biofilms. Pharmaceutics. 2021;13(3).
Rukavina Z, Vanić Ž. Current trends in development of liposomes for targeting bacterial biofilms. Pharmaceutics. 2016;8(2).
Wang DY, Van der Mei HC, Ren Y, Busscher L, Shi L. Lipid-based antimicrobial delivery-systems for the treatment of bacterial infections. Front Chem. 2020;7:872.
Placha D, Jampilek J. Chronic inflammatory diseases, anti-inflammatory agents and their delivery nanosystems. Pharmaceutics. 2021;13(1):64.
Ahamad N, Kar A, Mehta S, et al. Immunomodulatory nanosystems for treating inflammatory diseases. Biomaterials. 2021;274:120875.
Choubey S, Goyal S, Varughese LR, et al. Probing gallic acid for its broad spectrum applications. Mini Rev Med Chem. 2018;18(15):1283–93.
Locatelli C, Filippin-Monteiro FB, Centa A, Creczinsky-Pasa TB. Antioxidant, antitumoral and anti-inflammatory activities of gallic acid. In: Li G, editor. Handbook on gallic acid: natural occurrences, antioxidant properties and health implications. Nova Publishers; 2013. p. 1–23.
Kaczmarek B. Tannic acid with antiviral and antibacterial activity as a promising component of biomaterials—a minireview. Materials. 2020;13(14):3224.
Ninan N, Forget A, Shastri VP, Voelcker NH, Blencowe A. Antibacterial and anti-inflammatory pH-responsive tannic acid-carboxylated agarose composite hydrogels for wound healing. ACS Appl Mater Interfaces. 2016;8(42):28511–21.
Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):174–81.
Leuck AM. Left ventricular assist device driveline infections: recent advances and future goals. J Thorac Dis. 2015;7(12):2151–7.
Serbanescu MA, Apple CG, Fernandez-Moure JS. Role of resident microbial communities in biofilm-related implant infections: recent insights and implications. Surg Infect (Larchmt). 2023;24(3):258–64.
Jo EK. Interplay between host and pathogen: immune defense and beyond. Exp Mol Med. 2019;51(12):1–3.
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Ma, Y., Guo, L., Ying, J. et al. Exploring Liposomal Systems for Gallic Acid and Tannic Acid Delivery: Potential Strategies to Address Inflammation and Infections in Pediatric Ventricular Assist Device Recipients. J Pharm Innov 18, 2170–2181 (2023). https://doi.org/10.1007/s12247-023-09782-x
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DOI: https://doi.org/10.1007/s12247-023-09782-x