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Characteristics of δ-Aminolevulinic Acid Dehydratase of the Cold-Water Sponge Halisarca dujardinii

  • THE ROLE OF REDOX-DEPENDENT PROTEINS IN THE IMPLEMENTATION OF REDOX-REGULATION OF CELLS
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Abstract

δ-Aminolevulinic acid dehydratase (ALAD) is a key enzyme of the cytoplasmic heme biosynthesis pathway. The primary structure of the ALAD gene, the multimeric structure of the ALAD/hemB protein, and ALAD expression during the annual reproductive cycle were studied in the cold-water marine sponge Halisarca dujardinii. The results implicated the GATA-1 transcription factor and DNA methylation in regulating ALAD expression. Re-aggregation of sponge cells was accompanied by a decrease in ALAD expression and a change in the cell content of an active ALAD/hemB form. Further study of heme biosynthesis and the role of ALAD/hemB in morphogenesis of basal animals may provide new opportunities for treating pathologies in higher animals.

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ACKNOWLEDGMENTS

We are grateful to staff of the “The Arctic Circle” tourist center (Russia) for help in material collection.

The study employed equipment of the Core Research Facility of the Koltsov Institute of Developmental Biology.

Funding

This work was supported by a state contract with the Koltsov Institute of Developmental Biology (project no. 0088-2021-0008).

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Authors and Affiliations

  1. Koltsov Institute of Developmental Biology, Russian Academy of Sciences, 119334, Moscow, Russia

    O. I. Kravchuk, A. D. Finoshin, K. I. Adameyko, N. G. Gornostaev, A. I. Zhurakovskaya, V. S. Mikhailov & Yu. V. Lyupina

  2. Belozersky Institute of Physicochemical Biology, Moscow State University, 119992, Moscow, Russia

    K. V. Mikhailov

  3. Kharkevich Institute for Information Transmission Problems, Russian Academy of Sciences, 127051, Moscow, Russia

    K. V. Mikhailov

  4. Shemyakin and Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia

    R. H. Ziganshin

  5. Regulatory Genomics Research Center, Institute of Fundamental Medicine and Biology, Kazan Federal University, 420012, Kazan, Russia

    E. I. Shagimardanova

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  1. O. I. Kravchuk
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  2. A. D. Finoshin
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Correspondence to O. I. Kravchuk.

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Translated by T. Tkacheva

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Kravchuk, O.I., Finoshin, A.D., Mikhailov, K.V. et al. Characteristics of δ-Aminolevulinic Acid Dehydratase of the Cold-Water Sponge Halisarca dujardinii. Mol Biol 57, 1085–1096 (2023). https://doi.org/10.1134/S0026893323060109

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