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Long Noncoding RNAs MEG3, TUG1, and hsa-miR-21-3p Are Potential Diagnostic Biomarkers for Coronary Artery Disease

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Abstract

Peripheral blood biomarkers are of particular importance to diagnose certain diseases including coronary artery disease (CAD) due to their non-invasiveness. Investigating the expression of noncoding RNAs (ncRNAs) paves the way to early disease diagnosis, prognosis, and treatment. Consequently, in this research, we aimed to investigate a panel of ncRNAs as potential biomarkers in patients with coronary artery disease. Two different groups have been designed (control and CAD). All participants were subjected to interviews and clinical examinations. Peripheral blood samples were collected, and plasma was extracted. At the same time, target ncRNAs have been selected based on literature review and bioinformatic analysis, and later they underwent investigation using quantitative real-time PCR. The selected panel encompassed the long non-coding RNAs (lncRNAs) MEG3, TUG1, and SRA1, and one related microRNA (miRNA): hsa-miR-21-3p. We observed statistically significant upregulation in MEG3, TUG1, and hsa-miR21-3p in CAD patients compared to control participants (p-value < 0.01). Nevertheless, SRA1 exhibited downregulation with no statistical significance (p-value > 0.05). All ncRNAs under study displayed a significantly strong correlation with disease incidence, age, and smoking. Network construction revealed a strong relationship between MEG3 and TUG1. ROC analysis indicated high potentiality for hsa-miR-21-3p to be a promising biomarker for CAD. Moreover, MEG3 and TUG1 displayed distinguished diagnostic discrimination but less than hsa-miR-21-3p, all of them exhibited strong statistical significance differences between CAD and control groups. Conclusively, this research pinpointed that MEG3, TUG1, and hsa-miR-21-3p are potential biomarkers of CAD incidence and diagnosis.

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Funding

No funding was received for conducting this study.

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Authors and Affiliations

  1. Biotechnology and Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, 62511, Beni-Suef, Egypt

    M. Abdelgawad & A. E. Ahmed

  2. Genetics Unit, Department of Histology and Cell Biology, Faculty of Medicine, Suez Canal University, 41522, Ismailia, Egypt

    H. Y. Abdallah

  3. Center of Excellence in Molecular and Cellular Medicine, Faculty of Medicine, Suez Canal University, 41522, Ismailia, Egypt

    H. Y. Abdallah

  4. Department of Cardiology, Faculty of Medicine, Suez Canal University, 41522, Ismailia, Egypt

    A. Fareed

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  1. M. Abdelgawad
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  4. A. E. Ahmed
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Contributions

Hoda Y. Abdallah, Amr E. Ahmed and Mai Abdelgawad contributed to the study conception and design. Clinical diagnosis and interviews to participants done by Ahmed Fareed. Experimental work, material preparation, and data collection carried out by Mai Abdelgawad and Hoda Y. Abdallah. Mai Abdelgawad performed statistical and bioinformatic analyses. All authors analyzed and interpreted the patient data. All authors discussed the final results. Amr E. Ahmed and Hoda Y. Abdallah supervised the work. The first draft of the manuscript was written by Mai Abdelgawad. While Hoda Y. Abdallah, Ahmed Fareed, and Amr E. Ahmed edited and commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to H. Y. Abdallah.

Ethics declarations

The current study has been conformed with all institutional policies, and national rules, and was authorized by Suez Canal University Hospital (SCUH) Institutional Ethical Committee, Faculty of Medicine at Suez Canal University in Ismailia, Egypt prior to the start of the study. The study was conducted following the Declaration of Helsinki. The enrolled subjects in this study were recruited from the Suez Canal University Hospital with ethical consent number (4504). Prior to this study, both CAD cases and healthy volunteers who submitted their blood samples signed a written informed permission form, which displayed patient consent to interviews, sample collection, data access, and test result access.

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The authors declare that they have no conflicts of interest.

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Abdelgawad, M., Abdallah, H.Y., Fareed, A. et al. Long Noncoding RNAs MEG3, TUG1, and hsa-miR-21-3p Are Potential Diagnostic Biomarkers for Coronary Artery Disease. Mol Biol 57, 1186–1198 (2023). https://doi.org/10.1134/S0026893324010126

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