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Changes in the Activity of Antioxidant Systems of Escherichia coli under Phosphate Starvation

  • OXIDATIVE STRESS AND ANTIOXIDANT DEFENSE SYSTEMS
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Abstract—Changes in the activity of antioxidant systems in Escherichia coli during phosphate starvation have been studied. It is shown that starvation was accompanied by a decrease in the intensity of respiration, an increase in the rate of superoxide production, and a decrease in the level of ATP. Simultaneously, there was a decrease in H2O2 in the medium and a significant increase in the expression of the katG and katE genes which encode the HPI and HPII catalases, respectively. At the same time, there was no drop in the membrane potential, which may indicate the retention of normal membrane activity in starving cells. It has been shown for the first time that the transition of E. coli to phosphate starvation is accompanied by significant changes in the status of glutathione. The most important of these are associated with a decrease in the level of reduced glutathione in the medium (GSHout) and with a simultaneous increase in its content in the cytoplasm (GSHin), as well as a shift in the GSHin to oxidized glutathione form (GSSGin) ratio towards reductive values, and GSHout/GSSGout towards oxidative values. Among the mutants used in the work, the gor trxB double mutant, which is deficient in the synthesis of glutathione reductase and thioredoxin reductase, showed the most pronounced distinctive features. Compared to the parental strain, this mutant showed a multiple higher expression of katG::lacZ, the highest level of oxidized intra- and extracellular glutathione, and, accordingly, the lowest GSH/GSSG ratio in both compartments. In general, the data we obtained indicate that during phosphate starvation the interaction of the glutathione redox-system and regulons that control protection against reactive oxygen species creates conditions that allow maintaining the concentration of ROS below the toxic level. As a result, phosphate-starved E. coli cells can maintain high viability for a long period of time, which allows them to quickly resume growth after the addition of phosphate.

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Funding

The study was carried out with the financial support of the Russian Science Foundation (grant no. 22-14-00093).

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  1. Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch, Russian Academy of Sciences, 614081, Perm, Russia

    G. V. Smirnova, A. V. Tyulenev, N. G. Muzyka, L. V. Sutormina & O. N. Oktyabrsky

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Abbreviations. ROS, reactive oxygen species; GSH (L-γ-glutamyl-L-cysteinyl-glycine), reduced form of glutathione; GSSG, oxidized form of glutathione; in/out, in the cytoplasm/in the culture medium; SOD, superoxide dismutase.

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Smirnova, G.V., Tyulenev, A.V., Muzyka, N.G. et al. Changes in the Activity of Antioxidant Systems of Escherichia coli under Phosphate Starvation. Mol Biol 57, 965–977 (2023). https://doi.org/10.1134/S0026893323060171

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