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T-cell Immunoglobulin and Mucin Domain 3 in Circulating Monocytes as a Novel Biomarker for Coronary Artery Disease

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Abstract

The diagnostic role of T-cell immunoglobulin and mucin domain 3 (Tim-3) expression levels in circulating monocytes in coronary artery disease (CAD) remains to be determined. Here, we enrolled of 265 patients and isolated circulating monocytes from the blood of all participants. We found that the Tim-3 expression levels in monocytes were lower in CAD patients than in the control group. Spearman correlation analysis verified that the Tim-3 levels in monocytes were negatively correlated with the Gensini score and the number of coronary vessels. Multivariate logistic regression analysis showed that the Tim-3 levels in circulating monocytes were negatively correlated with CAD, severe CAD, and three-vessel CAD. The ROC curve showed that Tim-3 possessed high diagnostic value for CAD, severe CAD, and three-vessel CAD, with CAD prediction being the most significant of these values. In conclusion, Tim-3 in circulating monocytes is a novel biomarker for CAD.

Graphical Abstract

T-cell immunoglobulin and mucin domain 3 (Tim-3) in circulating monocytes as a novel biomarker for coronary artery disease.

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Data Availability

Data is available upon request.

Abbreviations

Tim-3:

 T-cell immunoglobulin and mucin domain 3

CAD:

Coronary artery disease

AS:

Atherosclerosis

WBCC:

White blood cell count

NEUT%:

Neutrophilic granulocyte percentage

LYMP%:

Lymphocyte proportion

MONO%:

Monocyte percentage

Cr:

Creatinine

ALT:

Alanine aminotransferase

AST:

Aspartate aminotransferase

LDH:

Lactic dehydrogenase

TC:

Total cholesterol

TG:

Triglyceride

LDL-C:

Low-density lipoprotein-cholesterol

HDL-C:

High-density lipoprotein-cholesterol

Fg:

Fibrinogen

FDP:

Fibrinogen degradation products

DD:

D-dimer

AT-III:

Antithrombin III

APTT:

Activated partial thromboplastin time

qPCR:

Quantitative real-time PCR

GAPDH:

Glyceraldehyde 3-phosphate dehydrogenase

ROC:

Receiver operating characteristic

AUC:

Area under the curve

BMI:

Body mass index

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Funding

This work was supported by the Health Commission of Jiangsu Province (grant number ZD2022016) and the Science and Technology Office of Nanjing Medical University (grant number NMU20210039).

Author information

Authors and Affiliations

  1. Department of Cardiology, The Second Affiliated Hospital of Nanjing Medical University, Nanjing, China

    Fangping Xiao, Zhiqiang Jia, Meng Liu, Xiaoxiao Chen, Zhan Gu, Yizhou Chen, Yi Li, Mingyue Chen & Mei Hong

  2. Department of Pathology, The Affiliated BenQ Hospital of Nanjing Medical University, Nanjing, China

    Lei Wang

Authors
  1. Fangping Xiao
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  2. Zhiqiang Jia
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  3. Lei Wang
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  4. Meng Liu
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  6. Zhan Gu
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  7. Yizhou Chen
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Contributions

M.H were responsible for designing the experiments. FPX, ZQJ, and L.W. performed the experiments. M.L., XXC and Z.G. collected data. All authors contributed to manuscript preparation and revision.

Corresponding author

Correspondence to Mei Hong.

Ethics declarations

Ethics Approval and Consent to Participate

The study was approved by the Ethics Committee of the Second Affiliated Hospital of Nanjing Medical University (approval no. 2022-KY-123–01). All patients signed a written informed consent before participating in the study.

Conflict of interest

The authors declare that they have no conflict of interest.

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Associate Editor Craig M. Stolen oversaw the review of this article.

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Supplementary Information

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Supplementary file1 (DOCX 26 KB)

12265_2023_10466_MOESM2_ESM.tif

Supplementary file2 Fig. S1. Correlation analysis of Tim-3 expression in circulating monocytes. a Association between Tim-3 expression and TG. b Association between Tim-3 expression and TC. c Association between Tim-3 expression and LDL-C. D Association between Tim-3 expression and Fg. e Association between Tim-3 expression and DD. f Association between Tim-3 expression and APTT. g Association between Tim-3 expression and PT. h Association between Tim-3 expression and INR. i Association between Tim-3 expression and Gensini score. TC: Total cholesterol; TG: Triglyceride; LDL-C: Low-density lipoprotein-cholesterol; Fg: Fibrinogen; DD: D-dimer; APTT: Activated partial thromboplastin time; PT: Prothrombin time; INR: International normalized ratio (TIF 8717 KB)

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Xiao, F., Jia, Z., Wang, L. et al. T-cell Immunoglobulin and Mucin Domain 3 in Circulating Monocytes as a Novel Biomarker for Coronary Artery Disease. J. of Cardiovasc. Trans. Res. (2023). https://doi.org/10.1007/s12265-023-10466-7

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