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Toxicity of benzyl paraben on aquatic as well as terrestrial life

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Abstract

Parabens are derivatives of alkyl esters of p-hydroxybenzoic acid and come in different classes. These compounds are primarily used as antimicrobial preservative agents in many commercial products, including cosmetics and pharmaceuticals. Accordingly, Benzyl paraben (BeP) is known to be a potential endocrine disruptor. The aim of this study was to determine the toxicity of benzyl paraben (BeP) on aquatic and terrestrial organisms, specifically Scenedesmus sp., Moina macrocopa, and Eisenia fetida. All the organisms were treated with different concentrations of BeP (0.025 mg/L and 1000 mg/L), and LC25, LC50, and LC90 values were used to measure the toxicity levels. Results showed the LC values of BeP for M. macrocopa (3.3 mg/L, 4.7 mg/L, 7.3 mg/L) and E. fetida (173.2 mg/L, 479.8 mg/L, 1062 mg/L), respectively. Toxicity tests on green algae (Scenedesmus sp.) were conducted, the green algae were subjected to various BeP concentration. At 50 mg/L of BeP, cell viability was reduced to 56.2% and the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide) assay indicated 35.4% viable cells. The chlorophyll value and the biochemical parameters of the algal cells were corroborative with the cell viability test. Lethal indices (LC50) for M. macrocopa and E. fetida were evaluated for their toxicity on biochemical properties and were found to be catalase (0.111 mg/L, 0.5 mg/L), lipid peroxidation (0.072 mg/L, 0.056 mg/L), and total protein (0.309 mg/L, 0.314 mg/L), respectively. Overall, this study demonstrated the toxic impact of BeP on non-target aquatic as well as terrestrial species.

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Acknowledgements

We sincerely thank REVA University for providing the research facilities for smooth conduction of research work.

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Authors and Affiliations

  1. Department of Biotechnology, School of Applied Sciences, REVA University, Bangalore, Karnataka, India

    Begum Mumtaz, Anju Nair & Prabhakar Mishra

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  1. Begum Mumtaz
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Conceptualization: PM. Data curation: AN and MB. Writing original draft preparation: AN and MB. Visualization: PM. Supervision: PM. Project administration: PM.

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Correspondence to Prabhakar Mishra.

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Mumtaz, B., Nair, A. & Mishra, P. Toxicity of benzyl paraben on aquatic as well as terrestrial life. Ecotoxicology 32, 1272–1284 (2023). https://doi.org/10.1007/s10646-023-02717-9

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