Abstract
A feature in neurodegenerative disorders is the loss of neurons, caused by several factors including oxidative stress induced by reactive oxygen species (ROS). In this work, static magnetic field (SMF) was applied in vitro to evaluate its effect on the viability, proliferation, and migration of human neuroblastoma SH-SY5Y cells, and on the toxicity induced by hydrogen peroxide (H2O2), tert-butyl hydroperoxide (tBHP), H2O2/sodium azide (NaN3) and photosensitized oxidations by photodynamic therapy (PDT) photosensitizers. The SMF increased almost twofold the cell expression of the proliferation biomarker Ki-67 compared to control cells after 7 days of exposure. Exposure to SMF accelerated the wound healing of scratched cell monolayers and significantly reduced the H2O2-induced and the tBHP-induced cell deaths. Interestingly, SMF was able to revert the effects of NaN3 (a catalase inhibitor), suggesting an increased activity of catalase under the influence of the magnetic field. In agreement with this hypothesis, SMF significantly reduced the oxidation of DCF-H2, indicating a lower level of intracellular ROS. When the redox imbalance was triggered through photosensitized oxidation, no protection was observed. This observation aligns with the proposed role of catalase in cellular proctetion under SMF. Exposition to SMF should be further validated in vitro and in vivo as a potential therapeutic approach for neurodegenerative disorders.
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E.C.G.F. is grateful for a CAPES fellowship: 88882.328247/2019-0. Finance Code 001. J.B.S. is grateful for a CAPES fellowship: 88887.517645/2020-00. Finance Code 001. H.U. is grateful for grant support from FAPESP (grant 2018/07366-4) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Project No. 406396/2021 and 308012/2021-6). D.F.S.P. is grateful for grant support from FAPESP (grant 2018/13492-2) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq Project No. 304017/2021).
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Frachini, E.C.G., Silva, J.B., Fornaciari, B. et al. Static Magnetic Field Reduces Intracellular ROS Levels and Protects Cells Against Peroxide-Induced Damage: Suggested Roles for Catalase. Neurotox Res 42, 2 (2024). https://doi.org/10.1007/s12640-023-00679-8
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DOI: https://doi.org/10.1007/s12640-023-00679-8