Abstract
Sauger (Sander canadensis) and walleye (Sander vitreus) are closely related North American fish species that are often managed by fishery agencies throughout their ranges. However, genotyping resources for sauger are presently limited to a small set of microsatellite loci. We evaluated whether primers in an existing walleye genotyping-in-thousands panel could amplify single nucleotide polymorphism loci (SNPs) in sauger. We identified 71 primer pairs that amplify 118 SNPs in both species. Allele frequency differences were large enough to confidently distinguish the species and identify hybrids. Additionally, we identified 41 loci with observed heterozygosity > 0.1 in sauger; these markers may be useful for simple population genetic analyses and parentage analysis when few contributors are present and for differentiating highly structured populations.
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Data Availability
Coauthor and data author, Paul Albosta, has made the GTscore primer-probe file and genotypes for walleye, sauger, and simulated saugeye available in an archived GitHub repository at https://github.com/palbosta/Winnebago_sauger_parentage (DOI https://doi.org/10.5281/zenodo.7683147). Questions about the data can be directed to the data author, Paul Albosta at palbosta@uwsp.edu.
References
Bootsma ML, Gruenthal KM, McKinney GJ et al (2020) A GT-seq panel for walleye (Sander vitreus) provides important insights for efficient development and implementation of amplicon panels in non-model organisms. Mol Ecol Resour 20:1706–1722. https://doi.org/10.1111/1755-0998.13226
Campbell NR, Harmon SA, Narum SR (2015) Genotyping-in-thousands by sequencing (GT-seq): a cost effective SNP genotyping method based on custom amplicon sequencing. Mol Ecol Resour 15:855–867. https://doi.org/10.1111/1755-0998.12357
Dray S, Dufour A (2007) The ade4 Package: implementing the duality diagram for ecologists. J Stat Softw 22:1–20. https://doi.org/10.18637/jss.v022.i04
Gelwicks G, Flammang M, Meerbeek J, Bowler M (2014) Walleye management plan
Gillenwater D, Granata T, Zika U (2006) GIS-based modeling of spawning habitat suitability for walleye in the Sandusky River, Ohio, and implications for dam removal and river restoration. Ecol Eng 28:311–323. https://doi.org/10.1016/j.ecoleng.2006.08.003
Graeb BDS, Willis DW, Billington N et al (2010) Age-Structured Assessment of walleyes, saugers, and naturally produced hybrids in three Missouri River reservoirs. North Am J Fish Manag 30:887–897. https://doi.org/10.1577/m09-091.1
Hansen GJA, Read JS, Hansen JF, Winslow LA (2017) Projected shifts in fish species dominance in Wisconsin lakes under climate change. Glob Chang Biol 23:1463–1476. https://doi.org/10.1111/gcb.13462
Hartman T, Tyson J, Page K, Stott W (2019) Evaluation of potential sources of sauger Sander canadensis for reintroduction into Lake Erie. J Great Lakes Res 45:1299–1309. https://doi.org/10.1016/j.jglr.2019.09.027
Hoff MH (2001) A rehabilitation plan for walleye populations and habitats in Lake Superior. Great Lakes Fishery Commission Misc. Publication 2003-01. Ann Arbor, MI
Jaeger ME, Zale AV, McMahon TE, Schmitz BJ (2005) Seasonal movements, habitat use, aggregation, exploitation, and entrainment of saugers in the Lower Yellowstone river: an empirical assessment of factors affecting population recovery. North Am J Fish Manag 25:1550–1568. https://doi.org/10.1577/m04-158.1
Jombart T (2008) Adegenet: a R package for the multivariate analysis of genetic markers. Bioinformatics 24:1403–1405. https://doi.org/10.1093/bioinformatics/btn129
Loukmas JJ (2013) Conservation management plan for sauger in New York State. Albany, New York
McKinney GJ, Pascal CE, Templin WD et al (2020) Dense SNP panels resolve closely related chinook salmon populations. Can J Fish Aquat Sci 77:451–461. https://doi.org/10.1139/cjfas-2019-0067
Meek MH, Larson WA (2019) The future is now: Amplicon sequencing and sequence capture usher in the conservation genomics era. Mol Ecol Resour 795–803. https://doi.org/10.1111/1755-0998.12998
Pegg MA, Layzer JB, Bettoli PW (1996) Angler exploitation of anchor-tagged saugers in the Lower Tennessee River. North Am J Fish Manag 16:218–222. https://doi.org/10.1577/1548-8675(1996)016<0218:aeoats>2.3.co;2
Quist MC, Stephen JL, Lynott ST et al (2010) An evaluation of angler harvest of walleye and saugeye in a Kansas reservoir. J Freshw Ecol 25:1–7. https://doi.org/10.1080/02705060.2010.9664351
Radomski P (2003) Initial attempts to actively manage recreational Fishery Harvest in Minnesota. North Am J Fish Manag 23:1329–1342. https://doi.org/10.1577/m01-231am
Schmalz PJ, Fayram AH, Isermann DA et al (2011) Harvest and exploitation. Biol Manag Cult Walleye Sauger 375–401
Thioulouse J, Dray S, Dufour A-B et al (2018) Multivariate analysis of ecological data with ade4. Springer New York, New York, NY
Acknowledgements
We thank the members of the Wisconsin Department of Natural Resources and Walleyes for Tomorrow for collecting samples used in this research. Any use of trade, firm, or product names is for descriptive purposes only and does not imply endorsement by the U.S. Government.
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This work was supported by funding from the Wisconsin Department of Natural Resources.
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All authors contributed to the study conception and design. Data collection was performed by Paul Albosta. Analyses were performed by Jared J. Homola. The first draft of the manuscript was written by Jared J. Homola and all authors commented on subsequent versions of the manuscript. All authors read and approved the final manuscript.
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Homola, J.J., Larson, W.A. & Albosta, P. An amplicon genotyping panel suitable for species identification and population genetics in sauger (Sander canadensis) and walleye (Sander vitreus). Conservation Genet Resour 16, 103–110 (2024). https://doi.org/10.1007/s12686-023-01329-z
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DOI: https://doi.org/10.1007/s12686-023-01329-z