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Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum

  • Microbial Physiology and Biochemistry
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Abstract

Escherichia coli RclA and Staphylococcus aureus MerA are part of the Group I flavoprotein disulfide reductase (FDR) family and have been implicated in the contribution to bacterial pathogenesis by defending against the host immune response. Fusobacterium nucleatum is a pathogenic, anaerobic Gram-negative bacterial species commonly found in the human oral cavity and gastrointestinal tract. In this study, we discovered that the F. nucleatum protein FN0820, belonging to the Group I FDR family, exhibited a higher activity of a Cu2+-dependent NADH oxidase than E. coli RclA. Moreover, FN0820 decreased the dissolved oxygen level in the solution with higher NADH oxidase activity. We found that L-tryptophan and its analog 5-hydroxytryptophan inhibit the FN0820 activities of NADH oxidase and the concomitant reduction of oxygen. Our results have implications for developing new treatment strategies against pathogens that defend the host immune response with Group I FDRs.

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Data availability

The data underlying the results are available as part of the article and further data are available from the corresponding author on reasonable request.

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Acknowledgements

This research was supported by the Bio & Medical Technology Development Program of the National Research Foundation (NRF) funded by the Ministry of Science & ICT (MSIT) (grant NRF-2019M3E5D6063871). This research was also supported by the Korea Institute of Planning and Evaluation for Technology in Food, Agriculture, Forestry and Fisheries (IPET), funded by the Ministry of Agriculture, Food and Rural Affairs (MAFRA) (321036053HD020).

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Authors and Affiliations

  1. Department of Agricultural Biotechnology and Research Institute of Agriculture and Life Sciences, CALS, Seoul National University, Seoul, 08826, Republic of Korea

    Hyunwoo Shin, Yeongjin Baek, Dukwon Lee, Yonghoon Kwon & Nam-Chul Ha

  2. Department of Bioengineering, College of Life Science, Dalian Minzu University, Dalian, 116600, People’s Republic of China

    Yongbin Xu

  3. Infectious Diseases Therapeutic Research Center, Korea Research Institute of Chemical Technology (KRICT), Daejeon, 34114, Republic of Korea

    Inseong Jo

  4. Center for Food and Bioconvergence, Seoul National University, Seoul, 08826, Republic of Korea

    Nam-Chul Ha

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  1. Hyunwoo Shin
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  2. Yeongjin Baek
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  3. Dukwon Lee
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  4. Yongbin Xu
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  5. Yonghoon Kwon
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  6. Inseong Jo
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  7. Nam-Chul Ha
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Contributions

YB and NCH designed the research. YB, JR, and JHP conceived and performed the experiments. SJ, IJ and NCH provided expertise and feedback. YB, JR, and NCH wrote the manuscript, and NCH secured funding.

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Correspondence to Inseong Jo or Nam-Chul Ha.

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Shin, H., Baek, Y., Lee, D. et al. Structural and Functional Analyses of the Flavoprotein Disulfide Reductase FN0820 of Fusobacterium nucleatum. J Microbiol. 61, 1033–1041 (2023). https://doi.org/10.1007/s12275-023-00095-9

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  • DOI: https://doi.org/10.1007/s12275-023-00095-9

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