1887

Abstract

. Various plasmid-mediated resistance genes have been reported in , but little is known about their global distribution features, evolution pattern and spread.

. The potential mobilization mechanisms of resistance plasmids in have been poorly explored.

. The aim of the study was to investigate the prevalence and diversity of plasmid-mediated resistance genes among isolates, and focus on the analysis of the features of the resistance plasmids from .

. The plasmids tested were sequenced using the Illumina HiSeq platform in conjunction with PCR and inverted PCR. The susceptibility of the host strains was determined by broth microdilution. The transfer of plasmids tested was conducted by electroporation. The sequence data were compared using bioinformatics tools and the data from our laboratory and the National Center for Biotechnology Information (NCBI) database.

. Nineteen plasmids were identified from our laboratory and these resistance plasmids were functional and transferable. Moreover, we clustered five types of genetic backbones of plasmids from and revealed the global distribution features of the plasmid-mediated resistance genes.

. This is the first report of the coexistence of (H)-bearing type I plasmid and (C)-bearing type II plasmid in one clinical isolate. In addition, this study provides the first view of the global distribution of plasmid-mediated resistance genes and classifies the plasmids in according to their backbone regions.

Funding
This study was supported by the:
  • the Outstanding Youth Foundation of He'nan Scientific Committee (Award 222300420043)
    • Principle Award Recipient: WangLei
  • National Key R&D Program of China (Award 2021YFD1301200)
    • Principle Award Recipient: JianheHu
  • National Natural Science Foundation of China (Award 32172862; 3197190095)
    • Principle Award Recipient: WangLei
  • the Leading Talents of Scientific and Technological Innovation in the Central Plains (Award 224200510024)
    • Principle Award Recipient: HuJianhe
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/content/journal/jmm/10.1099/jmm.0.001767
2023-12-19
2024-04-28
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