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Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells

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Abstract

Capra hircus (goat) induced pluripotent stem cells (giPSCs) harbor enormous scientific value and contribute to cellular agriculture, animal cloning, etc. Conventional approaches to giPSC generation suffer from complexity and low preparation efficiency. In the present study, we introduced the episomal vectors carrying the human pluripotent genes in goat somatic cells to generate the giPSC-like colonies. Initially, a simple non-enzymatic method was used to isolate the goat dermal fibroblast cells and, further, a cell line was established. Later, goat fibroblast cells were transfected with commercially available episomal vectors carrying the human pluripotent genes and successfully generated the iPSC-like colonies which exhibited the expression of goat endogenous pluripotent genes and positive staining with alkaline phosphatase. Moreover, giPS-like cells formed embryoid bodies (EBs)-like aggregates and weekly expressed the marker genes of two germ layers. Reprogramming of goat fibroblast using episomal vectors carrying human pluripotent genes could lead to the development of an efficient and time- and cost-effective approach to giPSC generation.

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Data availability

Data could be obtained upon reasonable request to the corresponding author.

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Funding

The funding agency SERB, Govt. of India (grant EEQ/2018/000114), provided financial support to the study.

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Authors and Affiliations

  1. Stem Cell Research Centre, Department of Hematology, Sanjay Gandhi Post Graduate Institute of Medical Sciences, Lucknow, 226014, India

    Anshuman Singh, Suraj Kumar Singh & Vinod Verma

  2. ICMR-National Institute for Research in Environmental Health, Bhopal, 462030, India

    Manoj Kumar, Devojit Kumar Sarma & Samradhi Singh

Authors
  1. Anshuman Singh
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  2. Suraj Kumar Singh
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  3. Manoj Kumar
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  4. Devojit Kumar Sarma
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  5. Samradhi Singh
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  6. Vinod Verma
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Corresponding author

Correspondence to Vinod Verma.

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The authors declare no competing interests.

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Singh, A., Singh, S.K., Kumar, M. et al. Establishment of Capra hircus somatic cells and induction of pluripotent stem-like cells. In Vitro Cell.Dev.Biol.-Animal 60, 3–8 (2024). https://doi.org/10.1007/s11626-023-00840-9

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  • DOI: https://doi.org/10.1007/s11626-023-00840-9

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