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Evidence of an active role of resveratrol derivatives in the tolerance of wild grapevines (Vitis vinifera ssp. sylvestris) to salinity

  • Regular Paper – Physiology/Biochemistry/Molecular and Cellular Biology
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Abstract

Resveratrol and its derivatives are the most important phytoalexins with a crucial role in plant defense mechanisms. These compounds can occur either naturally or in response to abiotic stresses. Among them, salinity is one of the major threats to the sustainability and productivity of agro-economically important species, particularly those involved in the vini-viticulture sector. Understating salinity tolerance mechanisms in plants is required for the development of novel engineering tools. This study aimed to investigate the potential role of resveratrol derivatives in salinity tolerance of wild grapevines. Our data revealed that the tolerant Tunisian wild grapevine genotype “Ouchtata” exhibited an increased accumulation of resveratrol derivatives (glycosylated and non-glycosylated resveratrol and t-ɛ-viniferin and hydroxylated t-piceatannol) in both stems and roots, along with an increased total antioxidant activity (TAA) compared to the sensitive genotype “Djebba” under stress conditions, suggesting an involvement of these stilbenes in redox homeostasis, thereby, protecting cells from salt-induced oxidative damage. Overall, our study revealed, for the first time, an active role for resveratrol derivatives in salt stress tolerance in wild grapevine, highlighting their potential use as metabolic markers in future grapevine breeding programs for a sustainable vini-viticulture in salt-affected regions.

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Data availability

The data that support the findings of this study are available from the corresponding author, [SD], upon reasonable request.

Abbreviations

TPC:

Total phenolic compounds

TFC:

Total flavonoid content

TAA:

Total antioxidant activity

ROS:

Reactive oxygen species

SER:

Shoot elongation rate

RWC:

Relative water content

MSI:

Membrane stability index

PCA:

Principal analysis component

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Acknowledgements

This research was conducted within the framework of the Tunisian National Research Program in the laboratory of Plant Molecular Physiology at the Centre of Biotechnology of Borj-Cedria. The project received financial support from the Tunisian Ministry of Higher Education and Scientific Research, and further assistance was provided through a scholarship granted by the Faculty of Sciences of Tunis, University Tunis El-Manar. The authors would like to thank the technical staff of the laboratory for their valuable contributions.

Funding

Université de Tunis El Manar, Ministère de l’Enseignement Supérieur et de la Recherche Scientifique.

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Authors and Affiliations

  1. Laboratory of Plant Molecular Physiology, Center of Biotechnology of Borj-Cedria, BP 901, 2050, Hammam-Lif, Tunisia

    Faouzia Hanzouli, Hassène Zemni, Mahmoud Gargouri, Ahmed Mliki & Samia Daldoul

  2. Faculty of Sciences of Tunis, University Tunis El-Manar, El Manar II, 2092, Tunis, Tunisia

    Faouzia Hanzouli

  3. Laboratory of Legumes and Sustainable Agrosystems, Centre of Biotechnology of Borj-Cedria, B.P 901, 2050, Hammam-Lif, Tunisia

    Hatem Boubakri

  4. Department of Agronomy, Food, Natural Resources, Animals and Environment (DAFNAE), University of Padova, Vialedell’Università, 16, 35020, Lesagnaro, PD, Italy

    Simone Vincenzi

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  1. Faouzia Hanzouli
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  2. Hassène Zemni
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  3. Mahmoud Gargouri
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  4. Hatem Boubakri
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  6. Simone Vincenzi
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  7. Samia Daldoul
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Contributions

SD, HZ, AM and MG conceived and designed the study. FH and SD conducted research including greenhouse culture and physiological analysis. FH performed biochemical and stilbene analysis. SV supervised HPLC analysis, SD and FH wrote the original draft of the manuscript with considerable contribution from MG and HB. All authors read and contribute to the review and editing of the final manuscript.

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Correspondence to Mahmoud Gargouri or Samia Daldoul.

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Hanzouli, F., Zemni, H., Gargouri, M. et al. Evidence of an active role of resveratrol derivatives in the tolerance of wild grapevines (Vitis vinifera ssp. sylvestris) to salinity. J Plant Res 137, 265–277 (2024). https://doi.org/10.1007/s10265-023-01515-y

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