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Structural elucidation of a non-starch polysaccharides from Lilii Bulbus and its protective effects against corticosterone-induced neurotoxicity in PC12 cells

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Abstract

Lilii Bulbus is a folk medicine for both culinary and medicinal purpose. In traditional medicine theory, Lilii Bulbus is usually used as an complementary therapy for nourishing the heart and lung, clearing heat in the treatment of mental instability and depression. In this study, NLPS-1a (Mw = 2610 Da, DP = 16), a water-soluble non-starch Lilii Bulbus polysaccharides, was isolated and purified. Structural analysis showed that NLPS-1a mainly contained Man and Glc with a molar ratio of 11.137 and 9.427. The glycosidic linkages of NLPS-1a were 1,3-Manp (59.93%), 1,2-Glcp (37.93%), T-Glcp (1.21%) and T-Manp (0.93%), indicating the highly-linear structures. In addition, NLPS-1a could significantly repair the injury of PC12 cells induced by corticosterone (CORT), reduce Lactate dehydrogenase (LDH) leakage and decrease the cell apoptosis in a dose-dependent manner. Above all, the results indicated that NLPS-1a had protective effects against CORT-induced neurotoxicity in PC12 cells, and might be a natural antidepressant, which enriched the study of the metabolic mechanism between herbal polysaccharides and antidepressant.

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All data included in this study are available upon request by contact with the corresponding author.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC) (no 82074017; 81573607; 81202917) and The Special Fund for Science and Technology Development in 2017 Guangdong Province of South China (no. 2017A030311031).

Funding

This work was supported by the National Natural Science Foundation of China (NSFC) (no 82074017; 81573607; 81202917) and The Special Fund for Science and Technology Development in 2017 Guangdong Province of South China (no. 2017A030311031).

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Authors and Affiliations

  1. The Center for Drug Research and Development, Guangdong Pharmaceutical University, 280# Waihuan East Road, Guangzhou Higher Education Mega Center, Guangzhou, Guangdong, 510006, P.R. China

    Yili Zheng, Xueying Li, Danna Lin, Jian Wu, Yufei Tian & Wen Rui

  2. Department of Pathogenic Biology and Immunology, School of Basic Medical Sciences , Guangdong Pharmaceutical University, Guangzhou, 510006, P.R. China

    Hongyuan Chen

  3. Key Laboratory of Digital Quality Evaluation of Chinese, Materia Medica of State Administration of TCM, Guangzhou, Guangdong, 510006, P.R. China

    Hongyuan Chen & Wen Rui

  4. Guangdong Provincial Key Laboratory of Advanced Drug Delivery, Guangdong Provincial Engineering Center of Topical Precise Drug Delivery System, Guangdong Pharmaceutical University, Guangzhou, Guangdong, 510006, China

    Wen Rui

  5. Guangdong Cosmetics Engineering & Technology Research Center, Guangzhou, 510006, P.R. China

    Hongyuan Chen

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  1. Yili Zheng
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Contributions

YZ: Investigation, Writing. XL: Investigation. DL: Software, Visualization. JW: Formal analysis. YT: Formal analysis. HC: Supervision, Writing - review & editing. WR: Conceptualization, Resources, Writing - review & editing.

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Correspondence to Hongyuan Chen or Wen Rui.

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Zheng, Y., Li, X., Lin, D. et al. Structural elucidation of a non-starch polysaccharides from Lilii Bulbus and its protective effects against corticosterone-induced neurotoxicity in PC12 cells. Glycoconj J 41, 57–65 (2024). https://doi.org/10.1007/s10719-023-10145-4

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