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Cloning and spatio-temporal expression of CsKr-h1 encoding the juvenile hormone response gene in Coccinella septempunctata L

Published online by Cambridge University Press:  05 January 2024

Ying Cheng Open the ORCID record for Ying Cheng [Opens in a new window] ,
Yuhang Zhou  and
Fengliang Li
Ying Cheng*
Affiliation:
Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guizhou, Guiyang 550006, China
Yuhang Zhou
Affiliation:
Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guizhou, Guiyang 550006, China
Fengliang Li
Affiliation:
Institute of Plant Protection, Guizhou Provincial Academy of Agricultural Sciences, Guizhou, Guiyang 550006, China
*
Corresponding author: Ying Cheng; Email: chying20000@126.com
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Abstract

The gene encoding juvenile hormone response (Krüppel homolog1, Kr-hl) in Coccinella septempunctata was investigated by cloning and analysing expression profiles in different developmental stages and tissues by quantitative real-time polymerase chain reaction (PCR). C. septempunctata Kr-hl (CsKr-hl) encoded a 1338 bp open reading frame (ORF) with a predicted protein product of 445 amino acids; the latter showed high similarity to orthologs in other species and contained eight highly-conserved Zn-finger motifs for DNA-binding. CsKr-hl was expressed in different developmental stages of C. septempunctata. The expression levels of CsKr-hl in eggs, 2nd, 3rd, 4th instar larvae, and pupa were 3.31, 2.30, 7.09, 0.58, and 7.48 times the number of 1st instar larvae, respectively. CsKr-hl expression levels in female adults gradually increased at 25–30 days and were significantly higher than expression at 1–20 days. CsKr-hl expression in 20–30 days-old male adults was significantly higher than males aged 1–15 days. CsKr-hl expression levels in heads of male and female adults were significantly higher than expression levels in the thorax, adipose, and reproductive system. Interestingly, CsKr-hl expression levels in the adipose and reproductive system of female adults were significantly higher than in adult male corresponding organs, which suggest that CsKr-hl plays an important role in regulating reproductive development in C. septempunctata.

Keywords

Coccinella septempunctatajuvenile hormoneKrüppel homolog1clonegene expression
Type
Research Paper
Information
Bulletin of Entomological Research , Volume 114 , Issue 1 , February 2024 , pp. 99 - 106
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Copyright
Copyright © The Author(s), 2024. Published by Cambridge University Press

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