Abstract
The aim of this study was to explore the effects of inoculating with different fungi on the structural characteristics of soil fungal communities and the nutrient absorption of blueberry plants. The fungi DSE (Phialocephala fortinii), SS (Penicillium pinophilum), ZB (Cladosporium cladosporioides), QMK (Chaetomium globosum), and LZ (Schizophyllum commune) were selected for this study. The soil fungal diversity and community structure in the blueberry rhizosphere were compared after inoculation with these different mycorrhizal fungi. The fungal diversity in the blueberry rhizosphere soil was significantly higher after inoculation with DSE, QMK, and ZB than after inoculation with SS and LZ. The dominant class, order, family, and genus of soil fungi were Eurotiomycetes (25.49–36.91%), Eurotiales (24.78–35.00%), Aspergillaceae (24.47–34.74%), and Penicllium (24.34–34.63%), respectively. Among all the fungal treatments, DSE led to the highest abundance of dominant classes, orders, families, and genera. Inoculation with SS, LZ, and DSE increased the contents of nitrogen, phosphorus, and potassium in blueberry plants. The uptake of nitrogen, phosphorus, and potassium were highest in DSE-inoculated plants. The nitrogen content was significantly higher in ZB-inoculated plants than in the control. A TOPSIS analysis showed that among the five fungi, DSE was the best strain to improve the diversity of the blueberry rhizosphere fungal community and promote nutrient uptake by blueberry plants, followed by LZ and ZB. These findings provide scientific data for the development and utilization of soil microbial resources, and provide useful information for improving the cultivation and production of blueberries.
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References
Addy HD, Piercey MM, Currah RS (2005) Microfungal endophytes in roots. Can J Bot 83(1):1–13. https://doi.org/10.1139/b04-171
Anderson IC, Campbell CD, Prosser JI (2010) Potential bias of fungal 18S rDNA and internal transcribed spacer polymerase chain reaction primers for estimating fungal biodiversity in soil. Environ Microbiol 5(1):36–47. https://doi.org/10.1046/j.1462-2920.2003.00383.x
Bacon CW, White JF (2016) Functions, mechanisms and regulation of endophytic and epiphytic microbial communities of plants. Symbiosis 68(1):87–98. https://doi.org/10.1007/s13199-015-0350-2
Bizabani C, Fontenla S, Dames JF (2016) Ericoid fungal inoculation of blueberry under commercial production in South Africa. Sci Hortic 209:173–177. https://doi.org/10.1016/j.scienta.2016.06.029
Chao HA, Qz A, Yc A, Cc A, Wwa B, Jh B, Xl A (2021) Colonization by dark septate endophytes improves the growth and rhizosphere soil microbiome of licorice plants under different water treatments. Appl Soil Ecol 166:103993. https://doi.org/10.1016/j.apsoil.2021.103993
Chen J, Deng Y, Yu X, Wu G, Gao Y, Ren A (2022) Epichloë endophyte infection changes the root endosphere microbial community composition of Leymus Chinensis under both potted and field growth conditions. Microb Ecol 84(1):1–14. https://doi.org/10.1007/s00248-022-01983-0
Clemmensen KE, Bahr A, Ovaskainen O, Dahlberg A, Ekblad A, Wallander H, Stenlid J, Finlay RD, Wardle DA, Lindahl BD (2013) Roots and associated fungi drive long-term carbon sequestration in boreal forest. Science (New York, NY) 339 (6127):1615–1618. http://science.sciencemag.org/
Fitzpatrick CR, Mustafa Z, Viliunas J (2019) Soil microbes alter plant fitness under competition and drought. J Evol Biol 32(5):438–450. https://doi.org/10.1111/jeb.13426
Gui LX, Lu SS, Chen Q, Yang L, Xiao JX (2021) iTRAQ-based proteomic analysis reveals positive impacts of arbuscular mycorrhizal fungi inoculation on photosynthesis and drought tolerance in blueberry. Trees 35(2):81–92. https://doi.org/10.1007/s00468-020-02015-5
Guo X, Wan Y, Shakeel M, Wang D, Xiao L (2021) Effect of mycorrhizal fungi inoculation on bacterial diversity, community structure and fruit yield of blueberry. Rhizosphere 19(11):100360. https://doi.org/10.1016/j.rhisph.2021.100360
Halifu S, Deng X, Song X, Song R (2019) Effects of two trichoderma strains on plant growth, rhizosphere soil nutrients, and fungal community of Pinus sylvestris var. mongolica annual seedlings. Forests 10 (9):758. https://doi.org/10.3390/f10090758
He C, Wang W, Hou J (2019) Plant growth and soil microbial impacts of enhancing licorice with inoculating dark septate endophytes under drought stress. Front Microbiol 10:2277. https://doi.org/10.3389/fmicb.2019.02277
Heijden M, Horton TR (2010) Socialism in soil? The importance of mycorrhizal fungal networks for facilitation in natural ecosystems. J Ecol 97(6):1139–1150. https://doi.org/10.1111/j.1365-2745.2009.01570.x
Huang Y, Song M, Guo Y, Wang Q, Guan W (2019) Variation and comprehensive evaluation of fruit and seed phenotypic traits of different mating combinations in Xanthoceras sorbifolium. Beijing Linye Daxue Xuebao/J Beijing Forestry Univ 41 (1):42–56. https://doi.org/10.13332/j.1000-1522.20180105
Illescas M, Rubio MB, Hernández-Ruiz V, Morán-Diez ME, Martínez de Alba AE, Nicolás C, Monte E, Hermosa R (2020) Effect of inorganic N top dressing and Trichoderma harzianum seed-inoculation on crop yield and the shaping of root microbial communities of wheat plants cultivated under high basal N fertilization. Front Plant Sci 11:575861. https://doi.org/10.3389/fpls.2020.575861.eCollection2020
Knapp K, Zajta (2015) Dark septate endophytic pleosporalean genera from semiarid areas. Persoonia 35(1):87–100. https://doi.org/10.3767/003158515X687669
Kosola KR, Workmaster BAA, Spada PA (2007) Inoculation of cranberry (Vaccinium macrocarpon) with the ericoid mycorrhizal fungus Rhizoscyphus ericae increases nitrate influx. New Phytol 176(1):184–196. https://doi.org/10.1111/j.1469-8137.2007.02149.x
Lata C, Gond SK, White JF (2018) Induction of abiotic stress tolerance in plants by endophytic microbes. Lett Appl Microbiol 66(4):268–276. https://doi.org/10.1111/lam.12855
Lifeng H, Jie L, Zhao X (2019) Dark septate endophytes improve the growth and the tolerance of Medicago sativa and Ammopiptanthus mongolicus under cadmium stress. Front Microbiol 10:3061–3061. https://doi.org/10.3389/fmicb.2019.03061
Liu BR, Zhang XZ, Hu TH, Li WJ (2013) Soil microbial diversity under typical vegetation zones along an elevation gradient in Helan Mountains. Acta Ecol Sin 33(22):7211–7220. https://doi.org/10.5846/stxb201208061110
Liu XM, Xu QL, Li QQ, Zhang H, Xiao JX (2017b) Physiological responses of the two blueberry cultivars to inoculation with an arbuscular mycorrhizal fungus under low-temperature stress. J Plant Nutr 40(18):2562–2570. https://doi.org/10.1080/01904167.2017.1380823
Liu LM, Gu ZW, Zeng YY (2017) Determination of total nitrogen in plant samples by Semimicro-Kjeldahl Method and uncertainty evaluation of measurement results. J Anhui Agric Sci 45 (34):7–946. https://doi.org/10.13989/j.cnki.0517-6611.2017.34.003
Wu LK, Huang WM, Wang JY, WU HM, Chen J, Qin XJ, Zhang ZY, Lin WX (2015) Diversity analysis of rhizosphere microflora of wild R. glutinosa grown in monocropping for different years. Acta Agrono Sinica 41(2):308–317. https://doi.org/10.3724/SP.J.1006.2015.00308
Lu Y, Qing QL, Yan Y, Qiang C, Xuan G, Jia XX (2020) Comparative transcriptome analysis reveals positive effects of arbuscular mycorrhizal fungi inoculation on photosynthesis and high-pH tolerance in blueberry seedlings. Trees 34(2):433–444. https://doi.org/10.1007/s00468-019-01926-2
Ma OM, Ma RG, Glick BR, Santoyo G (2018) Microbiome engineering to improve biocontrol and plant growth-promoting mechanisms. Microbiol Res 208:25–31. https://doi.org/10.1016/j.micres.2018.01.005
Massicotte HB, Melville LH, Peterson R. (2005) Structural characteristics of rootfungal interactions for five ericaceous species in eastern Canada. Can J Bot 83(8):1057–1064. https://doi.org/10.1139/b05-046
Newsham KK (2011) A meta-analysis of plant responses to dark septate root endophytes. New Phytol 190(3):783–793. https://doi.org/10.1111/j.1469-8137.2010.03611.x
Pawar PB, Melo JS, Kotkar HM, Kulkarni MV (2018) Role of indigenous mycorrhizal species in enhancing physiological and biochemical status, nutrient acquisition and yield pattern of groundnut (Arachis hypogaea L.). J Crop Sci Biotechnol 21(1):23–33. https://doi.org/10.1007/s12892-017-0075-0
Philippot L, Raaijmakers JM, Lemanceau P, Van dP, Wim H (2013) Going back to the roots: the microbial ecology of the rhizosphere. Nat Rev Microbiol 11(11):789–799
Read DJ (1983) The biology of mycorrhiza in the Ericales. Can J Bot 61(61):985–1004
Romanowicz KJ, Freedman ZB, Upchurch RA, Argiroff WA, Zak DR (2016) Active microorganisms in forest soils differ from the total community yet are shaped by the same environmental factors: the influence of pH and soil moisture. Fems Microbiol Ecol 92 (10):fiw149. https://doi.org/10.1093/femsec/fiw149
Saleem M, Law AD, Sahib MR, Pervaiz ZH, Zhang Q (2018) Impact of root system architecture on rhizosphere and root microbiome. Rhizosphere 6:47–51. https://doi.org/10.1016/j.rhisph.2018.02.003
Schmeisser C, Steele H, Streit WR (2007) Metagenomics, biotechnology with non-culturable microbes. Appl Microbiol Biotechnol 75(5):955–962. https://doi.org/10.1007/s00253-007-0945-5
Singh A, Lasek-Nesselquist E, Chaturvedi V, Chaturvedi S (2018) Trichoderma polysporum selectively inhibits white-nose syndrome fungal pathogen Pseudogymnoascus destructans amidst soil microbes. Microbiome 6(1):1–11. https://doi.org/10.1186/s40168-018-0512-6
Song XH, Li YK, Hu Y, Guo WD, Wu ZR, Zhang Y, Cao Z (2021) Endophytes from blueberry roots and their antifungal activity and plant growth enhancement effects. Rhizosphere 20:100454. https://doi.org/10.1016/j.rhisph.2021.100454
Stummer B, Zhang X, Yang H, Harvey P (2021) Co-inoculation of Trichoderma gamsii A5MH and Trichoderma harzianum Tr906 in wheat suppresses in planta abundance of the crown rot pathogen Fusarium pseudograminearum and impacts the rhizosphere soil fungal microbiome. Biol Control 165:104809. https://doi.org/10.1016/j.biocontrol.2021.104809
Tedersoo L, Bahram M, Põlme S, Kõljalg U, Yorou N (2014) Global diversity and geography of soil fungi. Science 346(6213):1256688. https://doi.org/10.1126/science.1256688
Urooj F, Farhat H, Ali SA, Ahmed M, Ehteshamul-Haque S (2018) Role of endophytic Penicillium species in suppressing the root rotting fungi of sunflower. Pak J Bot 50(4):1621–1628
van der Heijden MGA, de Bruin S, Luckerhoff L, Logtestijn SPR, Schlaeppi K (2016) A widespread plant-fungal-bacterial symbiosis promotes plant biodiversity, plant nutrition and seedling recruitment. ISME J 10(2):389–399. https://doi.org/10.1038/ismej.2015.120
Waqas M, Khan AL, Muhammad H, Shahzad R, Lee IJ (2015) Endophytic fungi promote plant growth and mitigate the adverse effects of stem rot: an example of Penicillium citrinum and Aspergillus terreus. J Plant Interact 10(1):280–287. https://doi.org/10.1080/17429145.2015.1079743
Yang WQ, Goulart BL, Demchak K, Li Y (2002) Interactive effects of mycorrhizal inoculation and organic soil amendments on nitrogen acquisition and growth of Highbush Blueberry. J Am Soc Horticult Sci Am Soc Horticult Sci 127(5):742–748
Yang YN, Ba L, Bai XN, Zhang LC, Wang DL (2010) An improved method to stain arbuscular mycorrhizal fungi in plant roots. Acta Ecol Sin 30(3):774–779
Zhang HH, Tang M, Chen H (2008) Diversity of soil microbial communities in the mycorrhizosphere of five afforestation tree species in the Loess Plateau. J Beijing For Univ 30(3):85–90. https://doi.org/10.3321/j.issn:1000-1522.2008.03.015
Acknowledgements
We are thankful to National Natural Science Foundation of China (31760205), Huizhou University PhD initiation Fund (Grant No. 2022JB021) and Guangdong Basic and Applied Basic Research Foundation of Guangdong Province-Yuehui Joint Foundation (Grant No. 2022A1515111095) for its financial support.
Funding
This research was funded by the National Natural Science Foundation of China (Grant No. 31760205), Huizhou University PhD initiation Fund (Grant No. 2022JB021) and Guangdong Basic and Applied Basic Research Foundation of Guangdong Province-Yuehui Joint Foundation (Grant No. 2022A1515111095).
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DlW, XlG: conceptualization. ZjS, LhX, DlW: methodology and writing-original draft preparation. ZjS, YzZ, XlA, YT and XyZ: software, formal analysis, and data curation. XlG and DlW: writing-review and editing. All authors have read and agreed to the published version of the manuscript.
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Song, Z., Xiao, L., Guo, X. et al. Effects of inoculating different mycorrhizal fungi on rhizosphere soil fungi and nutrient uptake of blueberry. Hortic. Environ. Biotechnol. 65, 29–41 (2024). https://doi.org/10.1007/s13580-023-00527-w
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DOI: https://doi.org/10.1007/s13580-023-00527-w