Abstract
The spikes of gramineous plants are composed of specialized units called spikelets. Two bracts at the spikelet bases are known as glumes. The spikelet glumes in barley are degenerated into threadlike structures. Here, we report a long glume mutant, lgm1, similar in appearance to a lemma with a long awn at the apex. Map-based cloning showed that the mutant lgm1 allele has an approximate 1.27 Mb deletion of in chromosome 2H. The deleted segment contains five putative high-confidence genes, among which HORVU.MOREX.r3.2HG0170820 encodes a C2H2 zinc finger protein, an ortholog of rice NSG1/LRG1 and an important candidate for the Lgm1 allele. Line GA01 with a long glume and short awn was obtained in progenies of crosses involving the lgm1 mutant. Interestingly, lsg1, a mutant with long glumes on lateral spikelets, was obtained in the progenies of the lgm1 mutant. The long glume variant increased the weight of kernels in the lateral spikelets and increased kernel uniformity across the entire spike, greatly improving the potential of six-rowed barley for malting.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank R.A. McIntosh, University of Sydney, Australia, and Mengjun Liu, Northwest A&F University, China, for the English editing.
Funding
This project was supported by the Natural Science Basic Research Program of Shaanxi (305310261022) and the Open Funds of the National Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agricultural University (ZW201703).
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Project design: ZLZ and YS; experimental work: ZLZ, PTZ, ZPZ, XGZ, and YS; data analysis: ZLZ, XYW, HYW, LPX, and YS; manuscript preparation: ZLZ, LPX, ZJQ, and YS. All authors have reviewed and approved the manuscript.
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Zhang, Z., Zhao, P., Wang, X. et al. Identification and map-based cloning of long glume mutant gene lgm1 in barley. Mol Breeding 44, 3 (2024). https://doi.org/10.1007/s11032-024-01448-x
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DOI: https://doi.org/10.1007/s11032-024-01448-x