Abstract
Pseudomonas spp., such as P. fluorescens group, P. fragi, and P. putida, are the major psychrophilic spoilage bacteria in the food industry. Bacteriophages (phages) are a promising tool for controlling food-spoilage and food-poisoning bacteria; however, there are few reports on phages effective on food-spoilage bacteria such as Pseudomonas spp. In this study, 12 Pseudomonas phages were isolated from chicken and soil samples. Based on the host range and lytic activity at 30 °C and 4 °C and various combinations of phages, phages vB_PflP-PCS4 and vB_PflP-PCW2 were selected to prepare phage cocktails to control Pseudomonas spp. The phage cocktail consisting of vB_PflP-PCS4 and vB_PflP-PCW2 showed the strongest lytic activity and retarded regrowth of P. fluorescens and P. putida at 30 °C, 8 °C, and 4 °C at a multiplicity of infection of 100. Nucleotide sequence analysis of the genomic DNA indicated that vB_PflP-PCS4 and vB_PflP-PCW2 phages were lytic phages of the Podoviridae family and lacked tRNA, toxin, or virulence genes. A novel endolysin gene was found in the genomic DNA of phage vB_PflP-PCS4. The results of this study suggest that the phage cocktail consisting of vB_PflP-PCS4 and vB_PflP-PCW2 is a promising tool for the biocontrol of psychrophilic food-spoilage pseudomonads during cold storage and distribution.
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Source of isolations was PCF1 from chicken fallopian tube, PCG1 from chicken gizzard; PCL1 from chicken liver; PCS1-7 from chicken skin, PCW2 from chicken wings; and PSP1 from soil. *Isolated from lettuce leaf
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This research was supported by JSPS KAKENHI (grant number JP23H02162).
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All authors contributed to the conception and design of this study. Takahisa Miyamoto contributed to the conceptualization, writing, review, editing, and supervision of the study. Material preparation, data collection, analysis, and the first draft of the manuscript were conducted by Daisuke Johno and Yu Zhang. Ken-ichi Honjoh and Yoshimitsu Masuda reviewed and edited the manuscript. Tahir Noor Mohammadi, Junxin Zhao, and Yunzhi Lin contributed to the methodology, investigation, and data curation. Aye Thida Maung, Chen Wang, Yuan Lu, Marwa Nabil Sayed Abdelaziz, Chen-Yu Lin, Mohamed El-Telbany, Su Zar Thi Lwin, and Catherine Hofilena Damaso performed visualization and investigation. All the authors have read and approved the final version of the manuscript.
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Johno, D., Zhang, Y., Mohammadi, T.N. et al. Characterization of selected phages for biocontrol of food-spoilage pseudomonads. Int Microbiol (2024). https://doi.org/10.1007/s10123-023-00479-2
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DOI: https://doi.org/10.1007/s10123-023-00479-2