Abstract
Diallyl disulfide (DADS) is effective at suppressing tumour cell growth and proliferation. This study verified the morphology and growth activity of MDCC-MSB-1 cells by using an MTT assay to detect the effect of DADS on the proliferation of MDCC-MSB-1 cells and a CCK8 assay to detect the effect of DADS on the viability and proliferation of MDCC-MSB-1 cells. We found that the viability and proliferation of MDCC-MSB-1 cells decreased with increasing DADS concentrations. MDC staining and Western blotting were used to analyse autophagy, the associated protein LC3 and the MEK/ERK pathway proteins MEK and ERK and to investigate changes in cellular autophagy based on cell morphology and molecular biology. With increasing concentrations of DADS, MDCC-MSB-1 cell autophagy increased in a gradient manner. Additionally, the conversion of the autophagy marker protein LC3-I increased with increasing drug concentrations, and the relative expression of LC3-II steadily increased, as did the expression of key protein components of the MEK/ERK signalling pathway, including P-MEK1/2 and P-ERK1/2. These results suggest that DADS induces autophagy through the MEK/ERK pathway, thereby inhibiting the proliferation of MDCC-MSB-1 cells.
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The authors confirmed that figures supporting the present study’s outcome were provided in the article [and/or its supplementary material].
Abbreviations
- DADS:
-
Diallyl disulfide
- MDCC-MSB-1:
-
Marek’s disease lymphoma-transformed cell line
- MEK:
-
Mitogen-activated protein kinase kinase
- ERK:
-
Extracellular regulated protein kinases
- MAPK:
-
Mitogen-activated protein kinases
- PI3K:
-
Phosphoinositide 3-kinase
- AKT:
-
Serine/threonine-protein kinase
- mTOR:
-
Mammalian target of rapamycin
- MTT:
-
Methyl thiazolyl tetrazolium
- CCK8:
-
Cell Counting Kit-8
- MDC:
-
Autophagy/Cytotoxicity Dual Stain Kit
- MD:
-
Marek’s Disease
- MDV:
-
Marek’s Disease virus
- LC3:
-
Microtubule-Associated Protein 1 Light Chain 3
- ATG7:
-
Recombinant Autophagy Related Protein 7
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The Hunan Provincial Department of Education (Grant No. 22A0164) provided funding for this work.
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Y.P. and C.J. designed the research and the manuscript. J.L., D.W., and P.Z. performed the cell culture, and cell autophagy assays. T.C. conducted and backed the cell incubations. C.J. and Y.T. provided the experimental apparatus and supported the research. The entire research process. All authors have sanctioned the definitive version of the manuscript.
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The authors confirmed that they followed the EU standards for the conservation and protection of animals intended for scientific use. The experimental design and procedures utilised in this investigation with the approval of the Animal Care and Use Committee of the Hunan Provincial Department of Education (22A0164).
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Pan, Y., Liu, J., Wang, D. et al. DADS Inhibits the Proliferation of MDCC-MSB-1 Cells by Inducing Autophagy via the MEK/ERK Signalling Pathway. Cell Biochem Biophys 82, 271–278 (2024). https://doi.org/10.1007/s12013-023-01214-4
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DOI: https://doi.org/10.1007/s12013-023-01214-4