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Tissue culture response and in vitro plant regeneration of ‘Haruka’ (Cerasus Sato-zakura Group ‘Haruka’), a new cultivar of Japanese flowering cherry

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Abstract

This study describes the in vitro regeneration of ‘Haruka’ plants, a new cultivar of Japanese flowering cherry registered in 2021 by the Japanese statutory authority. As this is a double-flowered cultivar produced by inter-specific hybridization, in vitro regeneration is an effective method for large-scale propagation. To promote proliferation, apical shoots were cultured on half-strength Murashige and Skoog medium supplemented with 5 μM 6-benzylaminopurine. The highest average of 7.8 shoots per explant was obtained at 15°C in the dark. Cultures were maintained in continuous darkness for 12 wk and then transferred to lighting conditions under a 16-h photoperiod at 25°C. Subsequently, a 100% rooting rate was obtained with the application of 1 μM indole-3-butyric acid in combination with 0.1 μM naphthaleneacetic acid. Additionally, the number of roots per shoot and the maximum length of roots were significantly higher under exposure to pink light illumination provided by cold cathode fluorescent lamps emitting red- and blue-colored light at a ratio of 80% and 20%, respectively. More than 95% of the regenerated plantlets survived after ex vitro acclimatization.

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References

  • Ainsley PJ, Collins GG, Sedgley M (2000) Adventitious shoot regeneration from leaf explants of almond (Prunus dulcis Mill.). In Vitro Cell Dev Biol-Plant 36:470–474

    Article  CAS  Google Scholar 

  • Akita M, Negishi K, Kitano A, Iwasaki M, Komae R, Ohta Y, Kuriu T, Takii T (2006) Mass propagation of cherry (Cerasus x yedoensis Matsum.) through shoot primordia. Act Hortic 725:579–584

    Article  CAS  Google Scholar 

  • Bajaj YPS (1989) Some Indian ornamental tree: Cassia fisula Linn., Ponciana regia (Boj.) and Jacaranda acutifolia Auct. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, Trees II, vol 5. Springer-Verlag, Berlin Heidelberg, pp 469–476

    Google Scholar 

  • Bretz F, Hothorn T, Westfall P (2010) Multiple comparisons using R. CRC press, Boca Raton, FL, USA

    Google Scholar 

  • Cavallaro V, Pellegrino A, Muleo R, Forgione I (2022) Light and plant growth regulators on in vitro proliferation. Plants 11:844

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cerović R, Ružić D (1987) Micropropagation of sour cherry (Prunus cerasus L.) cv. Šumadinka. Plant Cell Tiss Org Cult 9:151–157

    Article  Google Scholar 

  • Chée R (1986) In vitro culture of Vitis: the effects of light spectrum, manganese sulfate and potassium iodide on morphogenesis. Plant Cell Tiss Org Cult 7:121–134

    Article  Google Scholar 

  • Cheong EJ (2012) Biotechnological approaches for improvement and conservation of Prunus species. Plant Biotechnol Rep 6:17–28

    Article  Google Scholar 

  • Cheong EJ, Kim CS (2001) Study on the various conditions of in vitro culture for mass-propagation of Prunus yedoensis Matsumura. J Kor For Soc 90(2):184–189

    Google Scholar 

  • Chung U, Mack L, Yun JI, Kim S-H (2011) Predicting the timing of cherry blossoms in Washington, DC and Mid-Atlantic states in response to climate change. PloS One 6(11):e27439

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Cioć M, Szewczyk A, Żupnik M, Kalisz A, Pawlowska B (2018) LED lighting affects plant growth, morphogenesis and phytochemical contents of Myrtus communis L. in vitro. Plant Cell Tiss Org Cult 132:433–447

    Article  Google Scholar 

  • Dar HA, Singh SR, Sundouri AS, Sharma MK, Srivastava KK (2010) Micropropagation of sour cherry rootstocks by employing forced and unforced explants. Indian J Hort 67(Special Issue):9–14

    Google Scholar 

  • Dorić D, Ognjanov V, Ljubojević M, Barać G, Dulić J, Pranjić A, Dugalić K (2014) Rapid propagation of sweet and sour cherry rootstocks. Not Bot Horti Agrobo 42(2):488–494

    Article  Google Scholar 

  • Espinosa AC, Pijut PM, Michler CH (2006) Adventitious shoot regeneration and rooting of Prunus serotina in vitro cultures. Hort Sci 41(1):193–201

    CAS  Google Scholar 

  • Fuernkranz HA, Nowak CA, Maynard CA (1990) Light effects on in vitro adventitious root formation in axillary shoots of mature Prunus serotina. Physiol Plant 80:337–341

    Article  Google Scholar 

  • George EF, Davies W (2008) Effects of the physical environment. In: George EF, Hall M, De Klerk GJ (eds) Plant propagation by tissue culture, The background, vol 1, 3rd edn. Springer, Dordrecht, The Netherlands, pp 423–464

    Google Scholar 

  • Hung CD, Hong CH, Kim SK, Lee KH, Park JY, Nam MW, Choi DH, Lee HI (2016) LED light for in vitro and ex vitro efficient growth of economically important highbush blueberry (Vaccinium corymbosum L.). Acta Physiol Plant 38:152

    Article  Google Scholar 

  • Itahana N, Hashimoto K (2008) In vitro propagation of Prunus lannesiana cv. Horinji from axillary buds. Kanto Shinrin Kenkyu 59:121–124

    Google Scholar 

  • Joshi P, Trivedi R, Purohit SD (2009) Micropropagation of Wrightia tomentosa: effect of gelling agents, carbon source and vessel type. Indian J Biotecnol 8:115–120

    Google Scholar 

  • Kato S, Matsumoto A, Yoshimura K, Katsuki T, Iwamoto K, Tsuda Y, Ishio S, Nakamura K, Moriwaki K, Shiroishi T, Gojobori T, Yoshimaru H (2012) Clone identification in Japanese flowering cherry (Prunus subgenus Cerasus) cultivars using nuclear SSR markers. Breed Sci 62:248–255

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Katsuki T (2014) Flowering cherries in Japan. Gakken Education Publishing, Tokyo

    Google Scholar 

  • Katsuki T, Maruyama T (2016) Expand “Haruka”. In: Forestry and Forest Products Research Institute Quarterly Magazine No.32, pp 8–9

    Google Scholar 

  • Komakech R, Kim Y-G, Kim WJ, Omujal F, Yang S, Moon BC, Okello D, Rahmat E, Kyeyune GN, Matsabisa MG, Kang Y (2020) A micropropagation protocol for the endangered medicinal tree Prunus africana (Hook f.) Kalkman: genetic fidelity and physiological parameter assessment. Front. Plant Sci 11:548003

    Google Scholar 

  • Kozai T, Nishiuchi N, Fujiwara K, Watanabe I (1989) Effects of planting density in the vessel on the growth and net photosynthetic rates of potato plantlets in photoautotrophic micropropagation. J Jpn Soc Hortic Sci 58:244–245

    Google Scholar 

  • Kuitert W (1999) Japanese flowering cherries. Timber Press Inc., Portland, Oregon

    Google Scholar 

  • Kumar A, Sharma V, Thakur M (2020) In vitro rooting and hardening of clonal cherry rootstock Gisela 5 (Prunus cerasus x Prunus canescens). Indian J Agric Sci 90(5):1032–1035

    Article  Google Scholar 

  • Liu X, Pijut PM (2008) Plant regeneration from in vitro leaves of mature black cherry (Prunus serotina). Plant Cell Tiss Org Cult 94:113–123

    Article  Google Scholar 

  • Manivannan A, Soundararajan P, Halimah N, Ko CH, Jeong BR (2015) Blue LED light enhances growth, phytochemical contents, and antioxidant enzyme activities of Rehmannia glutinosa cultured in vitro. Hort Environ Biotechnol 56(1):105–113

    Article  CAS  Google Scholar 

  • Mansseri-Lamrioui A, Louerguioi A, Bonaly J, Yakoub-Bougdal S, Allili N, Gana-Kebbouche S (2011) Proliferation and rooting of wild cherry: the influence of cytokinin and auxin types and their concentration. African J Biotech 10(43):8613–8624

    Article  CAS  Google Scholar 

  • Maruyama E, Hosoi Y (2019) Progress in somatic embryogenesis of Japanese pines. Front Plant Sci 10:31

    Article  PubMed  PubMed Central  Google Scholar 

  • Maruyama E, Hosoi Y, Ishii K (2002) Somatic embryogenesis in Sawara cypress (Chamaecyparis pisifera Sieb. et Zucc.) for stable and efficient plant regeneration, propagation and protoplast culture. J For Res 7:23–34

    Article  CAS  Google Scholar 

  • Maruyama E, Ishii K, Kinoshita I, Ohba K, Saito A (1997) Micropropagation of Guazuma crinita Mart. by root and petiole culture. In Vitro Cell Dev Biol-Plant 33:131–135

    Article  Google Scholar 

  • Maruyama ET (2009) Polyethylene glycol improves somatic embryo maturation in big-leaf mahogany (Swietenia macrophylla King, Meliaceae). Bulletin of FFPRI 142:167–173

    Google Scholar 

  • Maruyama ET, Hosoi Y (2018) Micropropagation of Jacaranda (Jacaranda mimosaefolia D. Don). In: Bettaieb T (ed) Micropropagation of ornamental plants. Nova Science Publishers, Inc, New York, pp 89–114

    Google Scholar 

  • Maruyama ET, Ueno S, Hirayama S, Kaneeda T, Moriguchi Y (2020) Somatic embryogenesis and plant regeneration from Sugi (Japanese cedar, Cryptomeria japonica D. Don, Cupressaceae) seed families by marker assisted selection for the male sterility allele ms1. Plants 9:1029

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Matsuta N, Hirabayashi T, Akihama T (1983) Plantlet formation from leaf callus of Prunus lannesiana Wils. Japan J Breed 33(4):484–486

    Article  Google Scholar 

  • Maynard CA (1994) Six-year field test result of micropropagated black cherry (Prunus serotina). In Vitro Cell Dev Biol-Plant 30:64–69

    Article  Google Scholar 

  • McClelland MT, Smith MAL (1990) Vessel type, closure, and explant orientation influence in vitro performance of five woody species. HortScience 25:797–800

    Article  Google Scholar 

  • Morini S, Muleo R (2003) Effect of light quality on micropropagation of woody species. In: Jain SM, Ishii K (eds) Micropropagation of woody trees and fruits. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 3–35

    Google Scholar 

  • Muleo R, Thomas B (1997) Effects of light quality on shoot proliferation of Prunus cerasifera in vitro are the result of differential effects on bud induction and apical dominance. J Hort Sci 72(3):483–491

    Article  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco cultures. Physiol Plant 15:473–479

    Article  CAS  Google Scholar 

  • Nacheva L, Dimitrova N, Koleva-Valkova L, Stefanova M, Ganeva T, Nesheva M, Tarakanov I, Vassiliev A (2023) In vitro multiplication and rooting of plum rootstock ‘Saint Julien’ (Prunus domestica subsp. insititia) under fluorescent light and different LED spectra. Plants 12:2125

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Nacheva L, Dimitrova N, Koleva-Valkova L, Tarakanov I, Vassiliev A (2021) Effect of lighting on the growth of raspberry (Rubus idaeus L.) plants in vitro. Agric Sci 13:126–140

    Google Scholar 

  • Nishihara Y, Okada K (2013) Propagation of Cerasus jamasakura ‘Iyo-usuzumi’ by tissue culture. Ehimeken Rinken Kenpo 28:9–13

    Google Scholar 

  • Pérez-Tornero O, Ortín-Párraga F, Egea J, Burgos L (1999) Medium term storage of apricot shoot tips in vitro by minimal growth method. HortScience 34(7):1277–1278

    Article  Google Scholar 

  • Pevalek-Kozlina B (1990) Influence of the container size on the rate of Prunus avium shoot multiplication. Acta Bot Croat 49:47–52

    Google Scholar 

  • Poudel PR, Kataoka I, Mochioka R (2008) Effect of red- and blue-light-emitting diodes on growth and morphogenesis of grapes. Plant Cell Tiss Org Cult 92:147–153

    Article  Google Scholar 

  • Pruski K, Astatkie T, Nowak J (2005) Tissue culture propagation of Mongolian cherry (Prunus fruticosa) and Nanking cherry (Prunus tomentosa). Plant Cell Tiss Org Cult 82:207–211

    Article  Google Scholar 

  • Pruski KW, Lewis T, Astatkie T, Nowak J (2000) Micropropagation of chokecherry and pincherry cultivars. Plant Cell Tiss Org Cult 63:93–100

    Article  Google Scholar 

  • Ružić DV, Vujović TI (2008) The effects of cytokinin types and their concentration on in vitro multiplication of sweet cherry cv. Lapins (Prunus avium L.). Hort Sci (Prague) 35(1):12–21

    Article  Google Scholar 

  • Salazar GM (2022) The cherry blossom and its influence in Japanese culture. Japanese Society and Culture 4(12):155–165

    Google Scholar 

  • Sasaki Y (2003) Micropropagation of Prunus sargentii REHDER and P. verecunda (KOIDZ) Koehe. In: Jain SM, Ishii K (eds) Micropropagation of woody trees and fruits. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 507–518

    Chapter  Google Scholar 

  • Satoh T (1994) In vitro mass propagation from shoot-tip culture of Prunus sargentii. In: Bulletin Hokkaido Forestry Research Institute No. 31

    Google Scholar 

  • Satoh T (1999) In vitro mass propagation from shoot-tip culture of Prunus nipponica var. kurilensis Wil. Bulletin Hokkaido Forestry Research Institute No. 36

    Google Scholar 

  • Sedlák J, Paprštein F (2008) In vitro shoot proliferation of sweet cherry cultivars Karešova and Rivan. Hort Sci (Prague) 35(3):95–98

    Article  Google Scholar 

  • Sharma V, Thakur M, Kumar A (2017) An efficient method for in vitro propagation of Gisela 5 (Prunus cerasus x Prunus canescens) – clonal cherry rootstock. Int J Curr Microbiol App Sci 6(8):2617–2674

    Article  Google Scholar 

  • Tanaka M, Norikane A, Watanabe T (2009) Cold cathode fluorescent lamps (CCLF): revolutionary light source for plant micropropagation. Biotechnol & Biotechnol Eq 23(4):1497–1503

    Article  Google Scholar 

  • Tančeva Crmarić O, Kajba D (2016) Micropropagation of wild cherry (Prunus avium L.) from a clonal seed orchard. Šumarski list 5-6:273–282

    Google Scholar 

  • Thorpe TA, Harry IS, Kumar PP (1991) Application of micropropagation to forestry. In: Debergh PC, Zimmerman (eds) Micropropagation technology and application. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 311–336

    Chapter  Google Scholar 

  • Trivellini A, Toscano S, Romano D, Ferrante A (2023) LED lighting to produce high-quality ornamental plants. Plants 12:1667

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang YH, Rong JD, Fu Y, Chen LG, Cheng LY, Zheng YS (2015) Tissue culture and plant regeneration of Prunus campanulata Maxim. J Anim Plant Sci 25(3 Suppl. 1):146–151

    CAS  Google Scholar 

  • Zhang Z, Dai WH, Cheng ZM, Walla JE (2000) A shoot tip-culture micropropagation system for chokecherry. J Environ Hort 18(4):234–237

    Google Scholar 

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Acknowledgements

The authors thank the nursery personnel of the Forestry and Forest Products Research Institute (FFPRI, Tsukuba, Japan) for the support in the plant material preparation.

Funding

This work was supported by research grants from the Forestry and Forest Products Research Institute (FFPRI, Tsukuba, Japan).

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Authors and Affiliations

  1. Department of Forest Molecular Genetics and Biotechnology, Forestry and Forest Products Research Institute (FFPRI), Tsukuba, Japan

    Tsuyoshi E. Maruyama & Momi Tsuruta

  2. Kyushu Research Center, Forestry and Forest Products Research Institute (FFPRI), Kumamoto, Japan

    Toshio Katsuki

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  1. Tsuyoshi E. Maruyama
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  2. Momi Tsuruta
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  3. Toshio Katsuki
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Correspondence to Tsuyoshi E. Maruyama.

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Maruyama, T.E., Tsuruta, M. & Katsuki, T. Tissue culture response and in vitro plant regeneration of ‘Haruka’ (Cerasus Sato-zakura Group ‘Haruka’), a new cultivar of Japanese flowering cherry. In Vitro Cell.Dev.Biol.-Plant (2024). https://doi.org/10.1007/s11627-023-10407-8

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