Abstract
Cytochrome P450s are a large family of protein-encoding genes in plant genomes, many of which have not yet been comprehensively characterized. Here, a novel P450 gene, CYP82D47, was isolated and functionally characterized from cucumber (Cucumis sativus L.). Quantitative real-time reverse-transcription polymerase chain reaction analysis revealed that CYP82D47 expression was triggered by salicylic acid (SA) and ethephon (ETH). Expression analysis revealed a correlation between CYP82D47 transcript levels and plant defense responses against powdery mildew (PM) and Fusarium oxysporum f. sp. cucumerinum (Foc). Although no significant differences were observed in disease resistance between CYP82D47-RNAi and wild-type cucumber, overexpression (OE) of CYP82D47 enhanced PM and Foc resistance in cucumber. Furthermore, the expression levels of SA-related genes (PR1, PR2, PR4, and PR5) increased in CYP82D47-overexpressing plants 7 days post fungal inoculation. The levels of ETH-related genes (EIN3 and EBF2) were similarly upregulated. The observed enhanced resistance was associated with the upregulation of SA/ETH-signaling-dependent defense genes. These findings indicate the crucial role of CYP82D47 in pathogen defense in cucumber. CYP82D47-overexpressing cucumber plants exhibited heightened susceptibility to both diseases. The study results offer important insights that could aid in the development of disease-resistant cucumber cultivars and elucidate the molecular mechanisms associated with the functions of CYP82D47.
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Acknowledgements
We are grateful to Professor Yanju Zhang from Northeast Agricultural University for providing Strain H46-5 of the cucumber wilt pathogen.
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This work was supported by the Harbin Normal University Postgraduate Innovation Project (HSDBSCX2021-02) and Natural Science Foundation of Heilongjiang Province (LH2021C052).
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Conceptualization: H.-Y.W., X.-X.J. and G.-H. D.; writing—review and editing: H.-Y.W. and G.-H.D.; methodology: H.-Y.W., P.-F.L., Y.W., X.-X.J. and G.-H.D.; Software: H.-Y.W. and G.-H.D.; validation: H.-Y.W. and G.-H.D.; visualization: H.-Y.W., X.-X.J., C. -Y. C. and G.-H.D; writing—review and editing,: H.-Y.W., X.-X.J. and G.-H.D.; project administration: H.-Y.W., X.-X.J. and G.-H.D. Funding acquisition: H.-Y.W.,X.-X.J, and G.-H.D. All authors have read and agreed to the published version of the manuscript.
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Wang, Hy., Li, Pf., Wang, Y. et al. Overexpression of cucumber CYP82D47 enhances resistance to powdery mildew and Fusarium oxysporum f. sp. cucumerinum. Funct Integr Genomics 24, 14 (2024). https://doi.org/10.1007/s10142-024-01287-1
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DOI: https://doi.org/10.1007/s10142-024-01287-1