Abstract
We investigated the anther culture (AC) efficiency of nine genotypes of winter bread wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic callus, i.e., embryo-like structures (ELSs), green-, albino- and acclimatizated plantlets. The highest level of callus formation were shown for samples 120/20 (114.39 ELS per 100 anthers—ELS/100A) and 132/20 (16.26 ELS/100A). The number of green plantlets per 100 anthers (GP/100A) varied from 0 to 3.05 GP/100A with a mean of 0.71 GP/100A. The acclimatized plantlets (ADPs) per 100 regenerated green plantlets ranged in each combination, from 32.00 to 62.50 ADP/100GP with an average value of 35.92. Between 12.50 and 60.00 doubled haploid (DH) plants per 100 acclimatized plantlets (DH/100ADP), depending on the combination, with a mean of 29.41% were recovered. Fertile plants in the anthers culture 5 out of 9 studied genotypes were obtained. Seventeen dihaploid lines with complex rust resistance and common bunt (8–9 points) were obtained. Two clusters of complex resistance genes were identified in the ten studied DH lines: Lr26/Sr31/Pm8/Yr9 and Lr34/Yr18/Sr58/Pm38.
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Zambriborshch, I., Shestopal, O., Traskovetskaya, V. et al. Obtaining dihaploid lines of winter bread wheat with complex resistance to rust and common bunt by anther culture in vitro. CEREAL RESEARCH COMMUNICATIONS (2024). https://doi.org/10.1007/s42976-023-00466-3
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DOI: https://doi.org/10.1007/s42976-023-00466-3