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Genome-Wide Identification and Characterization of CHROMO Domain Family Genes Reveal Roles of the Maize Genes in Heat Stress Response

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Abstract

CMT2 and cpSRP43 are key factors in tolerance to heat stress, which have been revealed in Arabidopsis. It is largely unknown whether they play similar roles in heat stress resistance in monocotyledon species. Here we carried out genome-enabled identification of CHROMO domain genes in five monocots, to which both CMT2 and cpSRP43 belong. Results showed that gene copy numbers are similar in monocots to those in Arabidopsis. Phylogenetic analyses split the family into six major clades, among which CMT2 and cpSRP43 formed independent clades, respectively. Two CMT2s are present in the maize genome, while a single cpSRP43 exists in maize. Expression of the CTM2 homologs was elevated 2 and 48 h after heat stress in maize roots, while that of cpSRP43 was increased 2 and 48 h after stress in maize stalks. The genes identified here can be used as molecular targets for breeders to select for thermal-tolerant wheat lines, while phylogenetic and expression analyses of the CHROMO domain genes can be used as a knowledge base for molecular, biochemical and physiological characterization of the genes in monocots.

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Funding

This work is supported by Special Funds for Key Research Development and Promotion of Henan Province (222102110231) and the Key Research and Development Projects of Henan Province (231111113000).

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  1. Collaborative Innovation Center of Water Security for the Water Source Region of the Midline of South-to-North Diversion Project of Henan Province, Nanyang Normal University, 473001, Nanyang, Henan Province, China

    Pengfei Duan, Yuanmeng Kan, Shuqiong Yang & Jibao Chen

  2. State Key Laboratory of Vehicle Fuel Technology, Henan Tianguan Group Co., Ltd., 473002, Nanyang, Henan Province, China

    Pengfei Duan

  3. College of Geographic Sciences and Tourism Management, Nanyang Normal University, 473001, Nanyang, Henan Province, China

    Di Zhao

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Pengfei Duan, Kan, Y., Zhao, D. et al. Genome-Wide Identification and Characterization of CHROMO Domain Family Genes Reveal Roles of the Maize Genes in Heat Stress Response. Biol Bull Russ Acad Sci 50 (Suppl 3), S289–S297 (2023). https://doi.org/10.1134/S1062359022603494

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