Skip to main content
SpringerLink
Log in

Changes in Kinetic Parameters and Cytological Characteristics of Rooster Spermatozoa under the Influence of Technological Factors

  • ZOOTECHNY AND VETERINARY
  • Published:
Russian Agricultural Sciences Aims and scope

Abstract

The problems of fertility reducing of rooster semen in the cycle “native sperm–equilibration–short-term and long-term storage (cryopreservation)” are urgent. The purpose of this study was to determine the effect of different methods of preparation (centrifugation or filtration) of rooster semen on its quality characteristics, depending on the method of removing possible pollutions; to evaluate the change in the composition of the cytosol of spermatozoa of native sperm under the influence of dilution and during short-term storage. Materials and methods. Semen of roosters (n = 22) of the Russian white breed was used. Experiment 1: semen was divided into 3 aliquots: I—diluted with synthetic cryoprotective medium LCM in a ratio of 1:1, II—filtered semen diluted with medium (membrane pore diameter 0.2 μm), III—centrifuged (at 3000 rpm in for 10 minutes). Native and frozen/thawed sperm were evaluated in terms of damage to spermatozoa membranes, chromatin, and acrosomes. The composition of carbohydrates and polyols of native spermatozoa was assessed under the influence of dilution and after storage (3 h). The advantage of filtration as a method of technological preparation of semen compared to centrifugation in terms of progressive motility (with rectilinear-translational movement) of sperm (41.0% versus 27.0%) and chromatin damage (43.4% versus 66.4%) has been shown. The same advantage was observed in frozen/thawed sperm filtered before freezing in terms of progressive motility (25.5% vs. 5.5%) and chromatin damage—16.5% vs. 33.6%, respectively. Semen filtration, as a method of technological processing of rooster semen, can be an effective additional step in the preparation of semen for artificial insemination and/or short-term storage. The main component in the composition of the cytosol of native spermatozoa, according to the content of carbohydrates and polyols, was inositol —73.7% of Σ carbohydrates and polyols. The level of inositol decreased during storage by 6.5 times (from 0.030 to 0.007 mg/mL). The data obtained let us suppose the role of inositol as the main antioxidant in the cytosol of spermatozoa. Technological factors of storing rooster semen in various modes (short-term at a temperature of 5°C and long-term at a temperature of –196°C) have a significant impact on the ratio of sperm cytosol components (carbohydrates and polyols). A significant, 2.5‑fold decrease in the relative content of inositol in the cytosol of frozen/thawed spermatozoa, compared with the indicators of native semen, allows us to recommend the introduction of the antioxidant inositol into the composition of cryoprotective media for rooster semen.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1.
Fig. 2.
Fig. 3.

REFERENCES

  1. Zrimšek, P. and Manafi, M.E., Evaluation of a new method and diagnostic test in semen analysis, in Artificial Insemination in Farm Animals, Manafi, M.E., Ed., Rijeka, 2011, pp. 131–152.

    Google Scholar 

  2. GOST (State Standard) 27267-2017: Means of Reproduction. Freshly Obtained Undiluted Rooster and Turkey Sperm. Specifications, Moscow: Standartinform, 2017.

  3. Konopleva, A.P., Andreeva, A.A., and Trokholis, T.N., Sperm extenders and their impact on the effectiveness of artificial insemination, Sb. Nauchn. Tr. Vseross. Nauchno-Issed. Tekhnol. Inst. Ptitsevod., 2010, vol. 85, pp. 25–29.

    Google Scholar 

  4. Tselyutin, K.V. and Tur, B.K., Cryopreservation of bird sperm as a tool for preserving the gene pool, Genet. Razvedenie Zhivotn., 2015, no. 1, pp. 50–52.

  5. Peláez, J., Bongalhardo, D.C., and Long, J.A., Characterizing the glycocalyx of poultry spermatozoa: III. Semen cryopreservation methods alter the carbohydrate component of rooster sperm membrane glycoconjugates, Poult. Sci., 2011, vol. 90, no. 2, pp. 435–443.

    Article  PubMed  Google Scholar 

  6. Dos Santos, M.N., Ramachandran, R., Wamsley, K., et al., The Impact of in vitro inoculation and dietary supplementation with Bacillus subtilis on sperm quality of aged White Leghorn roosters, J. Appl. Poult. Res., 2018, no. 27, pp. 304–315.

  7. Kuroda, S., Yumura, Y., Mori, K., et al., Negative correlation between presence of reactive oxygen species and Sperm Motility Index in whole semen samples of infertile males, Rev. Int. Androl., 2017, vol. 15, no. 3, pp. 84–89.

    Google Scholar 

  8. Soler, L., Labas, V., Thélie, A., et al., Intact cell MALDI-TOF MS on sperm: A molecular test for male fertility diagnosis, Mol. Cell. Proteomics, 2016, vol. 15, no. 6, pp. 1998–2010.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  9. Santiago-Moreno, J. and Blesbois, E., Functional aspects of seminal plasma in bird reproduction, Int. J. Mol. Sci., 2020, vol. 21, no. 16, p. 5664. https://www.mdpi.com/1422–0067/21/16/5664. Cited April 16, 2023.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Li, Y., Sun, Y., Ni, A., et al., Seminal plasma proteome as an indicator of sperm dysfunction and low sperm motility in chickens, Mol. Cell. Proteomics, 2020, vol. 19, no. 6, pp. 1035–1046.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  11. Michailidis, G. and Avdi, M., Transcriptional profiling of gallinacins antimicrobial peptides in the chicken reproductive tract and embryos, J. Biol. Res., 2010, no. 14, pp. 211–218.

  12. Stanishevskaya, O., Silyukova, Y., Pleshanov, N., et al., Role of mono- and disaccharide combination in cryoprotective medium for rooster semen to ensure cryoresistance of spermatozoa, Molecules, 2021, vol. 26, no. 19, p. 5920. https://www.mdpi.com/1420–3049/26/19/5920. Cited April 10, 2023.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Tselyutin, K.V. and Tur, B.K., Iskusstvennoe osemenenie i kriokonservatsiya spermy sel’skokhozyaistvennoi ptitsy (petukhi, indyuki, gusaki, selezni) (Artificial Insemination and Cryopreservation of Poultry Sperm (Roosters, Turkeys, Geese, Drakes)), St. Petersburg: Vseross. Nauchno-Issled. Inst. Genet. Razved. S-kh. Zhivotn. Rossel’hozakad., 2013

  14. USSR Inventor’s Certificate No. 1130339, 1984.

  15. Silyukova, Yu.L., Stanishevskaya, O.I., Pleshanov, N.V., et al., Efficiency of using a combination of mono- and disaccharides in a diluent for freezing rooster semen, S‑kh. Biol., 2020, vol. 55, no. 6, pp. 1148–1158.

    Google Scholar 

  16. Stanishevskaya, O.I., Silyukova, Y., Fedorova, E., et al., Effects of trehalose supplementation on lipid composition of rooster spermatozoa membranes in a freeze/thaw protocol, Animals, 2023, vol. 13, no. 6, p. 1023. https://www.mdpi.com/2076–2615/13/6/1023. Cited April 10, 2023. https://doi.org/10.3390/ani13061023

    Article  PubMed  PubMed Central  Google Scholar 

  17. Pintado, B., de la Fuente, J., and Roldan, E.R.S., Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechst 33258, or to eosin: accuracy in the assessment of cell viability, J. Reprod. Fertil., 2000, vol. 118, no. 1, pp. 145–152. https://doi.org/10.1530/reprod/118.1.145

    Article  CAS  PubMed  Google Scholar 

  18. Beletti, M.E. and Mello, M.L.S., Comparison between the toluidine blue stain and the Feulgen reaction for evaluation of rabbit sperm chromatin condensation and their relationship with sperm morphology, Theriogenology, 2004, vol. 62, nos. 3–4, pp. 398–402.

    Article  CAS  PubMed  Google Scholar 

  19. Stanishevskaya, O., Silyukova, Y., Tereshina, V., et al., Trehalose as a stabilizer of the lipid composition of membranes and the composition of the cytosol of frozen/thawed rooster spermatozoa, Agriculture, 2023, no. 13, p. 1387. https://www.mdpi.com/2077–0472/13/7/1387. Cited April 12, 2023. https://doi.org/10.3390/agriculture13071387

  20. Carretero, M.I., Fumuso, F.G., Neild, D.M., et al., Evaluation of the acrosomal status in Lama glama sperm incubated with acrosome reaction inducers, Anim. Reprod. Sci., 2015, no. 160, pp. 1–11. https://www.sciencedirect.com/science/article/abs/pii/S0378432015001451?via%3Dihub. Cited April 12, 2023.

  21. Umezu, K., Kurata, S., Takamori, H., et al., Characteristics and possible role of bovine sperm head-tohead agglutination, Cells, 2020, vol. 9, no. 8, p. 1865. https://www.mdpi.com/2073–4409/9/8/1865. Cited April 10, 2023.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  22. Leahy, T., Rickard, J.P., Aitken, R.J., et al., Penicillamine prevents ram sperm agglutination in media that support capacitation, Reproduction, 2016, vol. 151, no. 2, pp. 167–177.

    Article  CAS  PubMed  Google Scholar 

  23. Amaral, A., Energy metabolism in mammalian sperm motility, WIREs Mech. Dis., 2022, no. 114, p. e1569. https://wires.onlinelibrary.wiley.com/doi/https://doi.org/10.1002/wsbm.1569. Cited April 14, 2023. 10.1002/wsbm.1569

  24. De Luca, M.N., Colone, M., Gambioli, R., et al., Oxidative stress and male fertility: role of antioxidants and inositols, Antioxidants, 2021, vol. 10, no. 8, p. 1283. https://www.mdpi.com/2076–3921/10/8/1283. Cited April 14, 2023.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references

Funding

This work was carried out within the framework of the theme of the State assignment of the Ministry of Education and Science of Russia no. 121052600357-8 “Study of biological mechanisms formation of productive and adaptive traits of domestic chickens (Gallus gallus domesticus) using physiological and biochemical, cytological, genetic and virological research methods in order to create new breeding forms.”

Author information

Authors and Affiliations

  1. Russian Research Institute of Farm Animal Genetics and Breeding, Branch of the L.K. Ernst Federal Research Center for Animal Husbandry, 196625, St. Peterburg, Russia

    Yu. L. Silyukova, E.  S. Fedorova & O. I. Stanishevskaya

Authors
  1. Yu. L. Silyukova
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. E.  S. Fedorova
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. O. I. Stanishevskaya
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Yu. L. Silyukova.

Ethics declarations

ETHICS APPROVAL AND CONSENT TO PARTICIPATE

The study was approved by the Ethical Commission of the RRIFAGB (protocol no. 11/2023 of 07.02.2023) in accordance with the accepted principles of bioethics art 5 part 2 European Convention for the protection of Vertebrate Animals for Experimental and Other Scientific purposes (ETS 123 1986).

CONFLICT OF INTEREST

The authors of this work declare that they have no conflicts of interest.

Additional information

Publisher’s Note.

Allerton Press remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Silyukova, Y.L., Fedorova, E.S. & Stanishevskaya, O.I. Changes in Kinetic Parameters and Cytological Characteristics of Rooster Spermatozoa under the Influence of Technological Factors. Russ. Agricult. Sci. 49, 664–669 (2023). https://doi.org/10.3103/S1068367423060150

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.3103/S1068367423060150

Keywords:

Search

Navigation