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Cactodera guizhouensis n. sp. (Nematoda: Heteroderinae), a new species of cyst-forming nematode parasitizing potato in Guizhou, China

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Abstract

A new cyst-forming nematode, Cactodera guizhouensis n. sp., isolated from the roots and surrounding soil of potato in Guizhou, China, is the first species of genus Cactodera found from the potato rhizosphere. The new species was characterized by the L/W ratio of its cyst, being 1.4 ± 0.1 (1.3–1.8), with a fenestral diameter of 17.7–28.7 × 12.3–23.4 μm and being absent vulval denticlest; the stylet length of second stage juveniles is 26.5 ± 1.2 (24.1–29.0) μm, while the tail is 53.4 ± 3.9 (45.0–61.9) μm and the hyaline tail is 25.7 ± 2.4 (21.6–29.8) μm, the lateral field having four incisures and areolation; the eggshell having punctations. The internal transcribed spacer sequences of ribosomal RNA (ITS-rRNA), and sequences of the D2-D3 region of 28S ribosomal RNA (28S-rRNA) from the new species were amplified and sequenced. The phylogenetic tree based on the sequences showed that the new species clustered into a separate clade and could be distinguished from other species in genus Cactodera. A key to the species of Cactodera is also provided in this study.

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Data Availability

All data generated or analysed during this study are included in the text. The sequences original data has been uploaded to the NCBI database (ID: OR438931-OR438934).

References

  • Baldwin, J. G., & Bell, A. H. (1985). Cactodera eremica n. sp., Afenestrata africana (Luc et al., 1973) n. gen., n. comb., and an emended diagnosis of Sarisodera Wouts and Sher, 1971 (Heteroderidae). Journal of Nematology, 17(2), 187–201.

  • Castresana, J. (2000). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Molecular Biology Evolution, 17(4), 540–552.

    Article  CAS  PubMed  Google Scholar 

  • Cid Del Prado Vera, I., Ferris, H., Nadler, S. A., & Argumedo, R. (2012). Four new species of Tripylina Brzeski, 1963 (Enoplida:Tripylidae) from Mexico, with an emended diagnosis of the genus. Journal of Nematode Morphology & Systematics, 15(2), 71–86.

    Google Scholar 

  • Cid Del Prado Vera,  I., & Subbotin, S. A. (2012). Belonolaimus maluceroi sp. N. (Tylenchida: Belonolaimidae) from a tropical forest in Mexico and key to the species of Belonolaimus. Nematropica, 42(2), 201–210.

    Google Scholar 

  • Cid Del Prado Vera, I., & Subbotin, S. A. (2014). A new cyst nematode, Cactodera torreyanae sp. n. (Tylenchida: Heteroderidae), parasitising romerito, Suaeda torreyana, in Texcoco Mexico. Nematology, 16(2), 163–174.

  • Cobb, N. A. (1918). Estimating the nema population of soil., with special reference to the sugar-beet and root-gall nemas, Heterodera schachtii Schmidt and Heterodera radicicold (Greef) Müller. Agric. Tech. Cir., 1, 1–48.

    Google Scholar 

  • De Ley, P., Felix, M. A., Frisse, L. M., Nadler, S. A., Sternberg, P. M., & Thomas, W. K. (1999). Molecular and morphological characterisation of two reproductively isolated species with mirror-image anatomy (Nematoda: Cephalobidae). Nematology, 1(6), 591–612.

    Article  Google Scholar 

  • Escobar-Avila, I. M., Subbotin, S. A., & Tovar-Soto, A. T. (2020). Cactodera solani n. sp. (Nematoda: Heteroderidae), a new species of cyst-forming nematode parasitising tomato in Mexico. Nematology, 23, 1–14.

    Article  Google Scholar 

  • Feng, Y. X., Wang, D., Xiao, D. X., Pereira, T. J., Xuan, Y. H., Wang, Y. Y., et al. (2018). Cactodera chenopodiae (Nematoda:Heteroderidae), a new species of cyst nematode parasitizing common lambsquarter (Chenopodium album) in Liaoning China. Zootaxa, 4407(3), 361–375.

    Article  PubMed  Google Scholar 

  • Golden, A. M., & Raski, D. J. (1977). Heterodera thornei n. sp.(Nematoda: Heteroderidae) and a review of related species. Journal of Nematology, 9(2), 93.

  • Graney, L. S. O., & Bird, G. W. (1990). Descriptions and comparative morphology of Cactodera milleri n. sp. (Nematoda:Heteroderidae) and Cactodera cacti with a review and key to the genus Cactodera. Journal of Nematology, 22(4), 457–480.

  • Handoo, Z. A., Skantar, A. M., Subbotin, S. A., Kantor, M. R., Hult, M. N., & Grabowski, M. (2021). Molecular and morphological characterization of a first report of Cactodera torreyanae Cid del Prado Vera & Subbotin, 2014 (Nematoda: Heteroderidae) from Minnesota, the United States of America. Journal of Nematology, 53(1), 1–5.

    Article  Google Scholar 

  • Huelsenbeck, J. P., & Ronquist, F. (2001). MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17(8), 754–755.

    Article  CAS  PubMed  Google Scholar 

  • Krall, E. L., & Krall, K. A. (1978). Revision of the plant nematodes of the family Heteroderidae on the basis of the trophic specialization of these parasites and their co-evolution with their host plants (pp. 39–56). Fitogel’mintologicheskie Issledovaniya, Moscow, USSR, Nauka

  • Li, W. H., Li, H. X., Ni, C. H., Shi, M. M., Wei, X. J., Liu, Y. G., et al. (2021a). A new cyst-forming nematode, Cactodera tianzhuensis n. sp.(Nematoda: Heteroderinae) from Polygonum viviparum in China with a key to the Genus. Journal of Nematology, 53(1), 1–15.

    Article  Google Scholar 

  • Li, X. J., Du, J. L., Zhou, J., Song, N. N., Ke, J. H., Jiao, D. C., et al. (2021b). Present situation and suggestion of potato industry development in Southwest China. South China Agriculture, 15(34), 129–133.

    Google Scholar 

  • Maafi, Z. T., Subbotin, S. A., & Moens, M. (2003). Molecular identification of cyst-forming nematodes (Heteroderidae) from Iran and a phylogeny based on ITS-rDNA sequences. Nematology, 5(1), 99–111.

    Article  CAS  Google Scholar 

  • Nylander, J. A. A. (2004). MrModeltest v.2.3 Evolutionary Biology Centre Uppsala University. Uppsala

  • Pan, C. S., Lin, J., & Xue, R. (1997). Description of Cactodera cacti and their observation by scanning electron microscope. Acta Parasitologica Et Medica Entomologica Sinica, 4(4), 214–217.

    Google Scholar 

  • Peng, D. L., & Vovlas, N. (1994). Occurrence of the cyst-forming nematode Cactodera thornei in China. Nematologia Mediterranea, 22(1), 75–78.

    Google Scholar 

  • Seinhorst, J. W. (1959). A rapid method for the transfer of nematodes from fixative to anhydrous glycerin. Nematologica, 4, 67–69.

    Article  Google Scholar 

  • Skantar, A. M., Handoo, Z. A., Kantor, M. R., Hafez, S. L., Hult, M. N., Kromroy, K., et al. (2021). First report of Cactodera milleri Graney and Bird, 1990 from Colorado and Minnesota. Journal of Nematology, 53, 1–7.

    Article  Google Scholar 

  • Standley, D. M. (2013). MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology Evolution, 30, 772–780.

    Article  PubMed  PubMed Central  Google Scholar 

  • Subbotin, S. A., Mundoocampo, M., & Baldwin, J. G. (2010). Systematics of Cyst Nematodes (Nematoda: Heteroderinae), Nematology Monographs and Perspectives 8A (Series Editors:Hunt, D.J. & Perry, R.N.). Leiden, The Netherlands, Brill

  • Subbotin, S. A., Akanwari, J., Nguyen, C. N., Del Prado, C., Vera, I., Chitambar, J. J., Inserra, R. N., et al. (2017). Molecular characterisation and phylogenetic relationships of cystoid nematodes of the family Heteroderidae (Nematoda: Tylenchida). Nematology, 19(9), 1065–1081.

    Article  Google Scholar 

  • Subbotin, S. A., Sturhan, D., Chizhov, V. N., Vovlas, N., & Baldwin, J. G. (2006). Phylogenetic analysis of Tylenchida Thorne, 1949 as inferred from D2 and D3 expansion fragments of the 28S rRNA gene sequences. Nematology, 8(3), 455–474.

    Article  CAS  Google Scholar 

  • Subbotin, S. A., Vierstraete, A., De Ley, P., Rowe, J., Waeyenberge, L., Moens, M., et al. (2001). Phylogenetic relationships within the cyst-forming nematodes (Nematoda, Heteroderidae) based on analysis of sequences from the ITS regions of ribosomal DNA. Molecular Phylogenetics, 21(1), 1–16.

    Article  CAS  Google Scholar 

  • Turner, S. J., & Subbotin, S. A. (2013). Cyst nematodes. In P. Nematology (Ed.), Perry RN & Moens (pp. 110–143). CAB International.

    Google Scholar 

  • Wang, K., Xie, H., Li, Y., Xu, C. L., Yu, L., & Wang, D. W. (2013). Paratylenchus shenzhenensis n. sp. (Nematoda: Paratylenchinae) from the rhizosphere soil of Anthurium andraeanum in China. Zootaxa, 3750(2), 167–175.

  • Xu, C. L., Zhao, C. B., Ding, S., Zhang, J. F., & Xie, H. (2016). A modified crude DNA preparation for direct PCR reaction of single plant-parasitic nematodes. Nematology, 18, 625–628.

    Article  CAS  Google Scholar 

Download references

Acknowledgements

This research was supported by the Detection and Control of Crop Disease and Pests Project of China (No. 101821301082351011). Thanks to Plant Protection and Phytosanitary Station of Guizhou for their help in the sample collection process.

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Author notes

  1. Chun-Hui Ni and Yu-Jia Xie contributed equally to this study.

Authors and Affiliations

  1. Laboratory of Plant Nematology and Research Center of Nematodes of Plant Quarantine, Department of Plant Pathology, College of Plant Protection, South China Agricultural University, Guangzhou, People’s Republic of China

    Chun-Hui Ni, Yu-Jia Xie, Si-Hua Yang, Chun-Ling Xu & Hui Xie

  2. Department of Plant Pathology, College of Agriculture, Guizhou University, Guiyang, 550025, People’s Republic of China

    Zai-Fu Yang

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  1. Chun-Hui Ni
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Correspondence to Chun-Ling Xu or Hui Xie.

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Ni, CH., Xie, YJ., Yang, SH. et al. Cactodera guizhouensis n. sp. (Nematoda: Heteroderinae), a new species of cyst-forming nematode parasitizing potato in Guizhou, China. Eur J Plant Pathol (2024). https://doi.org/10.1007/s10658-024-02816-1

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