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LiCOI1 mediates the biosynthesis of monoterpenes induced by methyl jasmonate in Lilium ‘Siberia’

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Abstract

As a volatile hormone signal, methyl jasmonate (MeJA) can regulate the biosynthesis of secondary metabolites. But in floral fragrance, the effect and mechanisms of MeJA on monoterpenes, the major components of many plants, are largely unknown. In this paper, we measured the release of monoterpenes from petals of Lilium ‘Siberia’ after MeJA treatment, as well as the expressions of monoterpene pathway genes and Lilium coronatine-insensitive (LiCOI1) gene. Then the LiCOI1 gene was cloned, and using virus-induced gene silencing (VIGS) technology, its function in monoterpene biosynthesis in response to MeJA was validated. The results showed that MeJA significantly improved the release amounts of myrcene, ocimene, and linalool. The expression levels of synthase genes [Lilium myrcene synthase (LiMYS), Lilium ocimene synthase (LiOCS), as well as Lilium linalool synthase (LiLIS)], two pathway genes [Lilium 1-deoxy-D-xylulose 5-phosphate synthase (LiDXS) and Lilium 1-deoxy-D-xylulose-5-phosphate reductoisomerase (LiDXR)] were enhanced. Moreover, the LiCOI1 gene exhibited a remarkably increased expression level, which suggested its signaling role. Based on the analysis, the full length of LiCOI1 gene was 1809 bp, whose deduced protein contained 602 amino acids and was mainly located in the nucleus. Silencing the expression of LiCOI1 in petals significantly depressed not only the gene expressions of LiMYS, LiOCS, LiLIS, LiDXS, and LiDXR, but also the release amounts of myrcene, ocimene, and linalool. These results confirm that LiCOI1 plays an important signaling role in MeJA-induced enhancement of monoterpene biosynthesis in Lilium ‘Siberia’.

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Acknowledgements

The work was supported by National Natural Science Foundation of China (No. 32372747) and the Beijing Municipal Education Commission for Their Financial Support through Innovative Transdisciplinary Program of Ecological Restoration Engineering.

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Authors and Affiliations

  1. Ancient Tree Health and Culture Engineering Technology Research Center, National Forestry and Grassland Administration / College of Landscape Architecture, Beijing University of Agriculture, Beijing, 102206, China

    Zhuo Leng, Ying-Ying Li, Jing Wu, Ping-Sheng Leng & Zeng-Hui Hu

  2. Beijing Engineering Research Center of Rural Landscape Planning and Design, Beijing University of Agriculture, Beijing, 102206, China

    Zhuo Leng, Ying-Ying Li, Yu-Chen Fu, Jing Wu, Ping-Sheng Leng & Zeng-Hui Hu

  3. Institute of Forestry New Technology, Chinese Academy of Forestry, Beijing, 100091, China

    Yu-Chen Fu

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  1. Zhuo Leng
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Contributions

PL and ZH conceived and designed the research project. ZL and YL performed the experiment. YF analyzed the data. ZL wrote this paper, and JW revised this paper. All authors read and approved the manuscript.

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Correspondence to Ping-Sheng Leng or Zeng-Hui Hu.

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Leng, Z., Li, YY., Fu, YC. et al. LiCOI1 mediates the biosynthesis of monoterpenes induced by methyl jasmonate in Lilium ‘Siberia’. Hortic. Environ. Biotechnol. 65, 303–312 (2024). https://doi.org/10.1007/s13580-023-00569-0

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