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Development of introgression lines and mapping of qGW2, a novel QTL that confers grain width, in rice (Oryza sativa L.)

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Abstract

Rice grain size is a key determinant of both grain yield and quality. Identification of favorable alleles for use in rice breeding may help to meet the demand for increased yield. In this study, we developed a set of 210 introgression lines (ILs) by using indica variety Huanghuazhan as the donor parent and erect-panicle japonica rice variety Wuyujing3R as the recurrent parent. A total of 133 ILs were selected for high-throughput sequencing. Using specific-locus amplified fragment (SLAF) sequencing technology, 10,103 high-quality SLAF labels evenly distributed on 12 chromosomes were obtained and selected for subsequent analysis. Using a high-density map, quantitative trait locus (QTL) mapping of grain size-related traits was performed, and a total of 38 QTLs were obtained in two environments. Furthermore, qGW2, a novel QTL that controls grain width on chromosome 2, was validated and delimited to a region of 309 kb via substitution mapping. These findings provide new genetic material and a basis for future fine mapping and cloning of favorable QTLs.

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Supplementary information is available for this paper. Correspondence and requests for materials and data should be addressed to H.Z.

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Funding

This study was financially supported by the National Key R&D Program of China (2023YFD1200904), Jiangsu Key R&D Program (BE2021301), Jiangsu Agriculture Science and Technology Innovation Fund (CX (23)3093), The Zhongshan Biological Breeding Laboratory (BM2022008-03), The open funds of the Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding (NO.PL202303), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Science and technology planning project of Nantong City, Jiangsu Province (MS12019064).

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  1. Xiangqiang Zhao, Zuopeng Xu, and YiBo Chen contributed equally to this paper.

Authors and Affiliations

  1. School of Life Sciences, Nantong University, Nantong, 226019, Jiangsu, China

    Xiangqiang Zhao

  2. Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding/Zhongshan Biological Breeding Laboratory/Key Laboratory of Plant Functional Genomics of the Ministry of Education, Agricultural College of Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Yuanyue Du, Meng Li, Benxi Huang, Yongshen Ge, Minghong Gu, Shuzhu Tang, Qiaoquan Liu & Honggen Zhang

  3. Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops/Jiangsu Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou, 225009, China

    Zuopeng Xu, Minghong Gu, Shuzhu Tang, Qiaoquan Liu & Honggen Zhang

  4. Guangdong Academy of Agricultural Sciences, Rice Research Institute, Guangzhou, 510640, Guangdong, China

    YiBo Chen

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  1. Xiangqiang Zhao
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Contributions

H.Z. conceived the study; X.Z, Z.X, and Y.C performed the experiments and analyzed the data with the assistance of Y.D, M.L, B.H, and Y.G; S.T, Q.L, and M.G. reviewed and edited the article; X.Z and H.Z wrote the article.

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Correspondence to Honggen Zhang.

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Zhao, X., Xu, Z., Chen, Y. et al. Development of introgression lines and mapping of qGW2, a novel QTL that confers grain width, in rice (Oryza sativa L.). Mol Breeding 44, 10 (2024). https://doi.org/10.1007/s11032-024-01453-0

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