Abstract
Grain size is an important appearance quality trait in rice, which also affects grain yield. In this study, a recombinant inbred line (RIL) population derived from a cross between indica variety 9311 and japonica variety Cypress was constructed. And 181 out of 600 RILs were sequenced, and a high-density genetic map containing 2842 bin markers was constructed, with a total map length of 1500.6 cM. A total of 10 quantitative trait loci (QTL) related to grain length (GL), grain width (GW), grain length-to-width ratio (LWR), and 1000-grain weight (TGW) were detected under two environments. The genetic effect of qGL4, a minor QTL for GL and TGW, was validated using three heterogeneous inbred family (HIF) segregation populations. It was further dissected into two closed linked QTL, qGL4.1 and qGL4.2. By progeny testing, qGL4.1 and qGL4.2 were successfully delimited to intervals of 1304-kb and 423-kb, respectively. Our results lay the foundation for the map-based cloning of qGL4.1 and qGL4.2 and provide new gene resources for the improvement of grain yield and quality in rice.
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Data availability
The data used in this study are available from the corresponding author on reasonable request.
Abbreviations
- QTL :
-
Quantitative trait loci
- RIL :
-
Recombinant inbred line
- GL :
-
Grain length
- GW :
-
Grain width
- LWR :
-
Grain length-to-width ratio
- TGW :
-
1000-Grain weight
- SNP :
-
Single nucleotide polymorphism
- GBS :
-
Genotyping-by-sequencing
- HIF :
-
Heterogeneous inbred family
- KASP :
-
Kompetitive allele-specific PCR
- LOD :
-
Logarithm of odds
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Acknowledgements
We would like to express our gratitude to Dr. Hao Zhou from Sichuan Agricultural University for helping us analyze the GBS data.
Funding
This work was supported by grants from the National Key R&D Program of China (2021YFD1200500), the Natural Science Foundation of Anhui Province (2008085QC120), the National Natural Science Foundation (31901529), the Major Scientific Technological Project of Anhui Province (202003a06020005 and 2021d06050002), and the National Rice Industry Technology System (CARS-01–063).
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PY and ZFL designed the research; PY and CJZ performed most of the experiments; TCM developed the RILs; JFX, KNZ, and YLW participated in part of agronomic trait statistics and field experiments. PY, CJZ, and ZFL performed data analysis and manuscript writing; all authors read and approved the final manuscript.
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Yun, P., Zhang, C., Ma, T. et al. Identification of qGL4.1 and qGL4.2, two closely linked QTL controlling grain length in rice. Mol Breeding 44, 11 (2024). https://doi.org/10.1007/s11032-024-01447-y
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DOI: https://doi.org/10.1007/s11032-024-01447-y