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Preparation of Polymer-Based Amino Acid Stationary Phase and Its Application for Mixed-Mode Chromatography

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Abstract

Polymer microspheres have received attention because of their excellent properties. In this work, a polymer matrix zwitterionic amino acid stationary phase was prepared. Polyglycidyl methacrylate divinylbenzene (PGMA-DVB) microspheres was used as the matrix and successful modification with L-phenylalanine by ring-opening reaction of epoxy groups on the surface of PGMA-DVB microsphere matrix. The stationary phase was characterized by scanning electron microscopy, Fourier-transform infrared spectra, and elemental analysis. The phenylalanine-modified stationary phase was used in RPLC/HILIC mixed-mode chromatography for the separation of alkylbenzenes, polycyclic aromatic hydrocarbons, phenols, nucleosides and nucleic acids and etc. as probes, respectively. The good spatial selectivity of the stationary phase was demonstrated by the separation of biphenyl isomers. The prepared stationary phase showed good stability in alkaline condition (pH = 10) and low swelling in organic solvent mobile phase. Meanwhile, the stationary phase also showed good performance in the separation of different vitamins (Rs > 5.3), and the separation and detection of PAHs in river water.

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Data Availability

All the data supporting the findings of this article are available in the manuscript and the electronic supplementary material.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Hanlin Zeng, Jingdong Peng, Huanjun Peng, Xiang Wang, Zilong Zhang, Hanqi Yang, Jiayu Yu & Jiajia Wu

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  1. Hanlin Zeng
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  2. Jingdong Peng
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Contributions

Hanlin Zeng: conceptualization, investigation, writing – original draft. Jingdong Peng: supervision, funding acquisition. Huanjun Peng: formal analysis, resources. Xiang Wang: Data curation. Zilong Zhang: software. Hanqi Yang: Visualization. Jiayu Yu: validation. Jiajia Wu: formal analysis

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Correspondence to Jingdong Peng.

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Zeng, H., Peng, J., Peng, H. et al. Preparation of Polymer-Based Amino Acid Stationary Phase and Its Application for Mixed-Mode Chromatography. Chromatographia 87, 147–157 (2024). https://doi.org/10.1007/s10337-024-04311-5

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