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Volume 73, Issue 2

, a trans-coding sRNA is involved in virulence modulation targeting mRNA in Typhimurium No Access

Abstract

Typhimurium (STM) is a food-borne Gram-negative bacterium, which can infect humans and a wide range of livestock and poultry, causing a variety of diseases such as septicaemia, enteritis and abortion.

We will decipher the impacts of sRNA on STM virulence and provide a theoretical basis to reveal the regulatory role and molecular mechanism of .

The main objective of this study was to clarify whether sRNA exerts regulatory roles on STM pathogenicity.

The gene was amplified and its molecular characteristics were analysed in this study. Then, gene deletion strain (STM-) and the complementary strain () were constructed by λ-Red homologous recombination method, respectively, to analyse of adhesion and invasive ability and pathogenicity of different strains. Subsequently, the potential target gene regulated by was predicted using target RNA2 software, followed by the verification of the interaction between and target mRNA using the dual plasmid reporter system (DPRS). Furthermore, the mRNA and protein level of target gene was determined using qRT-PCR and Western blot, respectively.

The results revealed that the cell adhesion and invasive ability and pathogenicity of STM- were significantly reduced compared to STM-SL1344 strain, indicating that the deficiency of gene significantly influenced STM pathogenicity. The DPRS results showed that can interact with the mRNA of target gene , thus suppressing the expression of gene. Furthermore, the level of mRNA was not influenced in STM-, but the expression of GldA protein decreased significantly.

Combining the bioinformatic analysis, these findings suggested that may bind to the SD sequence of mRNA, hindering the binding of ribosomes to mRNA, thereby inhibiting the expression of GldA protein to modulate the virulence of STM.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): modulation , Salmonella Typhimurium , sRNA , STnc1280 and virulence
Funding
This study was supported by the:
  • Young and middle-aged leading science and technology innovation talents plan of Xinjiang Corps (Award No. 2016BC001)
    • Principle Award Recipient: JunQiao
  • the International Science & Technology Cooperation Program of China (Award No. 2014DFR31310)
    • Principle Award Recipient: JunQiao
  • the national key research and development program (Award No. 2016YFD0500900)
    • Principle Award Recipient: JunQiao
© 2024 The Authors
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2024-02-14
2024-04-28
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STnc1280, a trans-coding sRNA is involved in virulence modulation via targeting gldA mRNA in Salmonella Typhimurium
J. Med. Microbiol. 73, 001804 (2024); https://doi.org/10.1099/jmm.0.001804
/content/journal/jmm/10.1099/jmm.0.001804
/content/journal/jmm/10.1099/jmm.0.001804
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