Abstract
Heading date is one of the important agronomic traits that affects rice yield. In this study, we cloned a new rice B3 family gene, OsL1, which regulates rice heading date. Importantly, osl1-1 and osl1-2, two different types of mutants of OsL1 were created using the gene editing technology CRISPR/Cas9 system and exhibited 4 days earlier heading date than that of the wild type under short-day conditions. Subsequently, the plants overexpressing OsL1, OE-OsL1, showed a 2-day later heading date than the wild type in Changsha and a 5-day later heading date in Lingshui, but there was no significant difference in other yield traits. Moreover, the results of subcellular localization study indicated that OsL1 protein was located in the nucleus and the expression pattern analysis showed that OsL1 gene was expressed in rice roots, stems, leaves, and panicles, and the expression level was higher at the root and weak green panicle. In addition, the OsL1 gene was mainly expressed at night time under short-light conditions. The transcriptomic analysis indicated that OsL1 might be involved in the Hd1-Hd3a pathway function. Together, our results revealed that the cloning and functional analysis of OsL1 can provide new strategy for molecular design breeding of rice with suitable fertility period.
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The data that support the findings of this study are available from the corresponding author upon reasonable request.
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Acknowledgements
We thank Yuelu Mountain Seed Industry Innovation Project (No. 2021NK1002) for funding support.
Funding
This study was supported by Yuelu Mountain Seed Industry Innovation Project (No. 2021NK1002), Huxiang Youth Talent Project (No.2020RC3069), Changsha Outstanding Innovative Youth Project (No. kq2107019), and the Changsha Science and Technology Project (No. kq2004053).
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Conceptualization and validation were done by YL and LZ, while formal analysis was performed by TW and HD. The investigation was carried out by YQ and SX, and MG and MQ provided the necessary resources. Data curation was performed by LL, and the original draft was prepared by LL. LL reviewed and edited the manuscript. Project administration was handled by SS and LL, while LLi and JW acquired the funding. All authors have read and agreed to the published version of the manuscript.
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Li, L., Qiu, M., Song, S. et al. Loss of function of OsL1 gene cause early flowering in rice under short-day conditions. Mol Breeding 44, 17 (2024). https://doi.org/10.1007/s11032-024-01444-1
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DOI: https://doi.org/10.1007/s11032-024-01444-1