Abstract
Pesticides, used in agriculture to control plant diseases, pose risks to the environment and human health. To address this, there's a growing focus on biocontrol, using microorganisms instead of chemicals. In this study, we aimed to identify Bacillus isolates as potential biological control agents. We tested 1574 Bacillus isolates for antifungal effects against pathogens like Botrytis cinerea, Fusarium solani, and Rhizoctonia solani. Out of these, 77 isolates formed inhibition zones against all three pathogens. We then investigated their lytic enzyme activities (protease, chitinase, and chitosanase) and the production of antifungal metabolites (siderophore and hydrogen cyanide). Coagulase activity was also examined to estimate potential pathogenicity in humans and animals. After evaluating all mechanisms, 19 non-pathogenic Bacillus isolates with significant antifungal effects were chosen. Molecular identification revealed they belonged to B. subtilis (n = 19) strains. The 19 native Bacillus strains, demonstrating strong antifungal effects in vitro, have the potential to form the basis for biocontrol product development. This could address challenges in agricultural production, marking a crucial stride toward sustainable agriculture.
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All data generated or analyzed during this study are included in this published article.
Abbreviations
- BCA(s):
-
Biological control agent(s)
- PGPR:
-
Plant growth promoting rhizobacteria
- HCN:
-
Hydrogen cyanide
- Bc:
-
Botrytis cinerea
- Fs:
-
Fusarium solani
- Rs:
-
Rhizoctonia solani
- PDA:
-
Potato dextrose agar
- NA:
-
Nutrient agar
- DNS:
-
Dinitrosalicylic acid
- CAS:
-
Chrome azurol S
- BLAST:
-
Basic Local Alignment Search Tool
- ANOVA:
-
Analysis of variance
- OGBa:
-
Operational group B.amyloliquefaciens
- NCBI:
-
National Center for Biotechnology Information
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Acknowledgements
Molecular identification of Bacillus isolates was performed in BMLab Danışmanlık Ltd. Şti., Ankara-Türkiye.
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This study is supported by the Ege University Scientific Research Projects Coordination Unit under project number FDK-2019–20159.
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DMG designed and performed the experiments and analyzed the data. RE encouraged DMG to investigate and supervised the findings of this work. DMG wrote the manuscript in consultation with RE. All authors discussed the results and contributed to the final manuscript.
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Maral-Gül, D., Eltem, R. Evaluation of Bacillus isolates as a biological control agents against soilborne phytopathogenic fungi. Int Microbiol (2024). https://doi.org/10.1007/s10123-024-00490-1
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DOI: https://doi.org/10.1007/s10123-024-00490-1