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Volume 73, Issue 2

High levels of multidrug-resistant isolates of genetically similar 1,4, [5],12:I:- from Brazil between 1983 and 2020 No Access

Abstract

1,4, [5],12:i:- strains with different antimicrobial resistance profiles have been associated with foodborne disease outbreaks in several countries. In Brazil, . 1,4, [5],12:i:- was identified as one of the most prevalent serovars in São Paulo State during 2004–2020.

However, few studies have characterized this serovar in Brazil.

This study aimed to determine the antimicrobial resistance profiles of . 1,4, [5],12:i:- strains isolated from different sources in Southeast Brazil and compare their genetic diversity.

We analysed 113 . 1,4, [5],12:i:- strains isolated from humans (=99), animals (=7), food (=5) and the environment (=2) between 1983 and 2020. Susceptibility testing against 13 antimicrobials was performed using the disc diffusion method for all the strains. Plasmid resistance genes and mutations in the quinolone resistance-determining regions were identified in phenotypically fluoroquinolone-resistant strains. Molecular typing was performed using enterobacterial repetitive intergenic consensus PCR (ERIC-PCR) for all strains and multilocus sequence typing (MLST) for 40 selected strains.

Of the 113 strains, 54.87 % were resistant to at least one antimicrobial. The highest resistance rates were observed against ampicillin (51.33 %), nalidixic acid (39.82 %) and tetracycline (38.05 %). Additionally, 39 (34.51 %) strains were classified as multidrug-resistant (MDR). Nine fluoroquinolone-resistant strains exhibited the mutation (Ser96→Tyr96) and contained the gene. The 113 strains were grouped into two clusters using ERIC-PCR, and most of strains were present in one cluster, with a genetic similarity of ≥80 %. Finally, 40 strains were typed as ST19 using MLST.

The prevalence of MDR strains is alarming because antimicrobial treatment against these strains may lead to therapeutic failure. Furthermore, the ERIC-PCR and MLST results suggested that most strains belonged to one main cluster. Thus, a prevalent subtype of 1,4, [5],12:i:- strains has probably been circulating among different sources in São Paulo, Brazil, over decades.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): antimicrobial resistance profiles , molecular typing and monophasic Salmonella
Funding
This study was supported by the:
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award 304803/2021-9)
    • Principle Award Recipient: JulianaPfrimer Falcao
  • Conselho Nacional de Desenvolvimento Científico e Tecnológico (Award Proc.304399/2018-3)
    • Principle Award Recipient: JulianaPfrimer Falcao
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award 88887.523672/2020-00)
    • Principle Award Recipient: Giovanado Nascimento Pereira
  • Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (Award 001)
    • Principle Award Recipient: JulianaPfrimer Falcao
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 2022/07013-0)
    • Principle Award Recipient: JulianaPfrimer Falcao
  • Fundação de Amparo à Pesquisa do Estado de São Paulo (Award 2019/19338-8)
    • Principle Award Recipient: JulianaPfrimer Falcao
© 2024 The Authors
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High levels of multidrug-resistant isolates of genetically similar Salmonella 1,4, [5],12:I:- from Brazil between 1983 and 2020
J. Med. Microbiol. 73, 001792 (2024); https://doi.org/10.1099/jmm.0.001792
/content/journal/jmm/10.1099/jmm.0.001792
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