Abstract
Galanin-like peptide (GALP) is a neuropeptide that was first isolated and identified from the porcine hypothalamus. Studies have described an anti-obesity effect of GALP. We previously found that intracerebroventricular administration of GALP in mice resulted in an increase in respiratory exchange rate 12 to 16 h later. GALP may also affect glucose metabolism, but the detailed mechanism has not been elucidated. In this study, we investigated the effects of GALP on glucose and lipid metabolism in the liver. Nine-week-old male C57BL / 6 J mice were administered a single intracerebroventricular dose of saline or GALP and dissected 16 h later. There were no significant between-group differences in body weight and blood glucose levels. With regard to gene and protein expression, G6Pase associated with hepatic gluconeogenesis was significantly reduced in the GALP group. In addition, the hepatokines selenoprotein P and fetuin-A, which induce insulin resistance in the liver, were significantly decreased in the GALP group. These results suggest that intracerebroventricular administration of GALP decreases the expression of key hepatokines, thereby enhancing glucose metabolism.
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Acknowledgements
We would like to thank Saki Asanuma and Yuumi Kawakami for their assistance with the analysis.
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This work was supported by JSPS KAKENHI Grant Number 21K11632(S.H.).
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S.H. contributed to the experimental design, conduct of experiments, interpretation of data, and writing of the manuscript. N.W. contributed to the experimental design, conduct of experiments and interpretation of data. YI contributed to the conduct of experiments and interpretation of data, T.H., F.T., H.K., N.Y. and N.K. contributed to the interpretation of data. S.S. contributed to the experimental design and project administration. All authors reviewed and approved the final manuscript.
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Hirako, S., Wada, N., Iizuka, Y. et al. Effect of Intracerebroventricular Administration of Galanin-Like Peptide on Hepatokines in C57BL/6 J Mice. J Mol Neurosci 74, 25 (2024). https://doi.org/10.1007/s12031-024-02200-y
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DOI: https://doi.org/10.1007/s12031-024-02200-y